Effect of triterpene saponins from roots of Ampelozizyphus amazonicus Ducke on diuresis in rats

Diniz, Lúcio Ricardo Leite; Santana, Paula Carvalho; Ribeiro, Ana Paula Araújo Ferreira; Portella, Viviane Gomes; Pacheco, Lílian Fernanda; Meyer, Nádia Burkowski; César, Isabela Costa; Cosenza, Gustavo Pereira; Brandão, Maria das Graças Lins; Vieira, Maria Aparecida Ribeiro

doi:10.1016/j.jep.2009.03.006

Cited by 14 publications

(17 citation statements)

References 23 publications

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“…In this study, SAP Aa D was tested at the dose of 50 mg/kg because it resulted in an approximate 60 % reduction in the diuresis induced by the oral loading of 0.9 % NaCl (4 ml/100 g body weight) in normal rats. This reduction was reported in our previous study [8] in which we have shown that SAP Aa D at dose varying from 25 to 1000 mg/kg reduced the urine production in a dose-dependent manner. As 60 % reduction neither represent the minimum nor the maximum effect of SAP Aa D, 50 mg/kg was the dose of choice to perform the experiments in the present study.…”

Section: Resultssupporting

confidence: 88%

“…Moreover, SAP Aa D abolished the diuretic effect of furosemide, a classical diuretic that affects renal Na + -ATPases [32]. In a previous study, our group reported that the oral administration of SAP Aa D exhibited a dose-dependent antidiuretic effect in normal rats in either dehydrated or hydrated conditions [8]. In the present study, SAP Aa D exhibited a similar effect even when administered 30 min prior to an oral loading of physiological solution (0.9 % NaCl).…”

Section: Discussionmentioning

confidence: 99%

“…The extraction, isolation and chemical characterization of SAP Aa D were performed according to previously described procedures [8]. Powdered roots of A. amazonicus were successively extracted by percolation with 70 % ethanol, with the solvent then evaporated to dryness (6.6 % w/w).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous studies, we have shown that a triterpene saponin mixture isolated from the roots of A. amazonicus Ducke (SAP Aa D), reduced the urine excretion of normal rats in a dose-dependent manner [8]. There is a need for novel oral antidiuretics to treat diseases, such as pituitary diabetes insipidus and nephrogenic diabetes insipidus; however, investigations for compounds other than the typically used arginine vasopressin (AVP) or desmopressin are lacking.…”

Section: Introductionmentioning

confidence: 99%

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The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

Diniz

Portella

Cardoso

et al. 2012

BMC Complement Altern Med

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BackgroundIn a previous study, we showed that a saponin mixture isolated from the roots of Ampelozizyphus amazonicus Ducke (SAPAaD) reduces urine excretion in rats that were given an oral loading of 0.9 % NaCl (4 ml/100 g body weight). In the present study, we investigated whether atrial natriuretic peptides (ANP) and renal ATPases play a role in the SAPAaD- induced antidiuresis in rats.MethodsTo evaluate the effect of SAPAaD on furosemide-induced diuresis, Wistar rats (250-300 g) were given an oral loading of physiological solution (0.9 % NaCl, 4 ml/100 g body weight) to impose a uniform water and salt state. The solution containing furosemide (Furo, 13 mg/kg) was given 30 min after rats were orally treated with 50 mg/kg SAPAaD (SAPAaD + Furo) or 0.5 ml of 0.9 % NaCl (NaCl + Furo). In the SAPAaD + NaCl group, rats were pretreated with SAPAaD and 30 min later they received the oral loading of physiological solution. Animals were individually housed in metabolic cages, and urine volume was measured every 30 min throughout the experiment (3 h). To investigate the role of ANP and renal Na+ pumps on antidiuretic effects promoted by SAPAaD, rats were given the physiological solution (as above) containing SAPAaD (50 mg/kg). After 90 min, samples of urine and blood from the last 30 min were collected. Kidneys and atria were also removed after previous anesthesia. ANP was measured by radioimmunoassay (RIA) and renal cortical activities of Na+- and (Na+,K+)-ATPases were calculated from the difference between the [32P] Pi released in the absence and presence of 1 mM furosemide/2 mM ouabain and in the absence and presence of 1 mM ouabain, respectively.ResultsIt was observed that SAPAaD inhibited furosemide-induced diuresis (at 90 min: from 10.0 ± 1.0 mL, NaCl + Furo group, n = 5, to 5.9 ± 1.0 mL, SAPAaD + Furo group n = 5, p < 0.05), increased both Na+-ATPase (from 25.0 ± 5.9 nmol Pi.mg-1.min-1, control, to 52.7 ± 8.9 nmol Pi.mg-1.min-1, p < 0.05) and (Na+,K+)-ATPase (from 47.8 ± 13.3 nmol Pi.mg-1.min-1, control, to 79.8 ± 6.9 nmol Pi .mg-1.min-1, p < 0.05) activities in the renal cortex. SAPAaD also lowered urine ANP (from 792 ± 132 pg/mL, control, to 299 ± 88 pg/mL, p < 0.01) and had no effect on plasma or atrial ANP.ConclusionWe concluded that the SAPAaD antidiuretic effect may be due to an increase in the renal activities of Na+- and (Na+,K+)-ATPases and/or a decrease in the renal ANP.

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

Diniz

Portella

Cardoso

et al. 2012

BMC Complement Altern Med

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“…suas cascas são utilizadas no preparo de um tônico fortificante (MeB 1993) e afrodisíaco (Berg 1993;santos et al 2005), e em casos de mordidas de cobras (Brandão et al 1992), no tratamento de gastrite, dores nas juntas, "inflamações de mulher", anemia e diabetes, como revitalizador, depurativo e diurético (Oliveira 2004). Recentemente, foi comprovado em um estudo in vivo o efeito diurético da saR em ratos (Diniz et al 2009). …”

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Estudo etnofarmacognóstico da saracuramirá (Ampelozizyphus amazonicus Ducke), uma planta medicinal usada por comunidades quilombolas do Município de Oriximiná-PA, Brasil

et al. 2011

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, suzana Guimarães leitãO 6 ResumoUm levantamento etnobotânico realizado em comunidades quilombolas de Oriximiná, Pará, destacou a saracuramirá (saR), Ampelozizyphus amazonicus Ducke, com vasto uso popular no tratamento da malária, como tônica e depurativa. Por este motivo, o presente trabalho objetivou realizar um estudo etnofarmacognóstico da saR nas respectivas comunidades. Por meio de uma análise etnobotânica quantitativa, foi verificado que saR apresentou-se dentre as 10 espécies mais versáteis pela elevada importância relativa (1,3), dentre as cinco espécies com maior importância cultural pelo elevado índice de saliência (0,311) e a espécie com maior concordância de uso principal para malária (85,7%). Uma análise do índice de espuma e do índice de hemólise para saR demonstra a presença de saponinas com elevado índice de espuma (833) e uma baixa atividade hemolítica (CH 50 2,6 mg ml -1 ). Para realizar uma análise das agliconas das saponinas de saR, a bebida preparada pelo método tradicional quilombola (BMt) foi hidrolisada e, após reação com diazometano, foi analisada por cromatografia gasosa. Dois sinais majoritários foram caracterizados por espectrometria de massas, um referente a um triterpeno de esqueleto damarânico, característico das saponinas da saR, e outro referente ao éster metílico do ácido betulínico. Partindo das informações de uso popular da saR, foi avaliada in vitro a atividade inibidora da acetilcolinesterase. apesar de BMt não ter mostrado atividade neste ensaio, é possível supor que as indicações de uso desta planta pelos quilombolas como fortificante e contra malária podem estar relacionadas a uma possível atividade adaptógena e imunoestimulante, dada à presença das saponinas e do ácido betulínico em BMt. PalavRas-Chave: etnofarmacologia, efeito adaptógeno, malária, saponinas, ácido betulínico. Ethnopharmacology Study of Saracuramirá (Ampelozizyphus amazonicusDucke) in the "Quilombola" communities of Oriximiná, Pará State, Brazil abstRaCt in an ethnobotanical survey conducted within "Quilombola" communities of Oriximiná, Pará state, "saracuramirá" (saR), Ampelozizyphus amazonicus Ducke, stood out as one of the most cited species with wide popular use, especially for malaria, as tonic and depurative. The aim of this paper was to carry out an ethnopharmacognostic study of saR in these communities. Quantitative ethnobotanical analysis of the data showed that saR stands out as one of the 10 most versatile species with a high relative importance (1,3); it remains among the five species with the greatest cultural importance, demonstrated by the high salience index (0,311), being the species with the highest major use agreement to malaria (85,7%). analysis of the foam and hemolysis indexes of saR shows the presence of saponins with high foam index (833) and low hemolytic activity (HD 50 2,6 mg ml -1). in order to analyze saR saponin aglycones, the drink was prepared by the traditional quilombola method (BMt), was hydrolyzed, and analyzed by gas chromatography after reaction with diazometha...

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Bioassay‐Guided Fractionation of Anti‐Leishmania Amazonensis Extracts from Ampelozizyphus Amazonicus by High‐Speed Countercurrent Chromatography

Mendonça,

Rangel de Paula,

Soares

et al. 2024

Chemistry & Biodiversity

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Ampelozizyphus amazonicus Ducke, known as "saracura‐mirá" in the Amazon rainforest, is valued for its traditional use in malaria prevention and treatment. The plant's roots and bark are employed for these purposes, while the wood is often overlooked. Given the global importance of leishmaniasis, research focused on A. amazonicus anti‐Leishmania amazonensis potential. The ethyl acetate extract from the bark (EAEB) exhibited the most effective inhibition of intracellular amastigote growth with IC50 7.0 µg.mL‐1 but showed high toxicity (CC50 9.0 µg.mL‐1). The wood ethanol (EW) and ethyl acetate (EAEW) extracts demonstrated promising results against L. amazonensis with IC50 15.8 µg.mL‐1 and 10.0 µg.mL‐1, CC50 50.5 µg.mL‐1 and 22.7 µg.mL‐1, respectively. High‐speed countercurrent chromatography combined with mass spectrometry off‐line detection was employed for the bioassay‐guided fractionation of EAEB and EAEW using HEMWat as solvent system. These extracts showed to be rich in saponins and triterpenes, besides minor glycosylated flavonoids. HSCCC was effective in obtaining high purity fractions, leading to the identification of a di‐glycosylated triterpene saponin from EAEB, and melaleucic acid from EAEW. These findings highlight A. amazonicus as a potential source for developing new therapeutic alternatives for leishmaniasis and HSCCC as a technique enabling better separation efficiency of complex saponin matrices.

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Effect of triterpene saponins from roots of Ampelozizyphus amazonicus Ducke on diuresis in rats

Cited by 14 publications

References 23 publications

The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

Estudo etnofarmacognóstico da saracuramirá (Ampelozizyphus amazonicus Ducke), uma planta medicinal usada por comunidades quilombolas do Município de Oriximiná-PA, Brasil

Bioassay‐Guided Fractionation of Anti‐Leishmania Amazonensis Extracts from Ampelozizyphus Amazonicus by High‐Speed Countercurrent Chromatography

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