Effect of tris(3-hydroxy-4-pyridinonate) iron(III) complexes on iron uptake and storage in soybean (Glycine max L.)

Santos, Carla S.; Carvalho, S.M.P.; Leite, Andreia; Moniz, Tânia; Roriz, Mariana; Rangel, António O. S. S.; Rangel, Maria; Vasconcelos, Marta W.

doi:10.1016/j.plaphy.2016.04.050

Cited by 28 publications

(30 citation statements)

References 77 publications

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“…Thus, the higher expression levels of this gene in Fe sufficient soybean and barrel medic plants are understandable (Figures 4 and 5) and are coherent with previous studies (Santos et al, 2016). This protein manages the insolubility and potential toxicity of Fe in the presence of oxygen, being involved in oxidative protection by sequestering free Fe (Lobreaux et al, 1995).…”

Section: Resultssupporting

confidence: 88%

“…On the contrary, M. truncatula roots overexpressed FRO2-like gene under Fe deficiency (Figure 5), as previously obtained in A. thaliana (Robinson et al, 1999), tomato (Li et al, 2004) and soybean (Santos et al, 2016). When Fe was present in sufficient amounts, M. truncatula had almost null FRO2-like expression ( Figure 5); since from the beginning of the trial, plants were in optimal conditions, they captured sufficient Fe to meet their daily requirements, thus inhibiting FRO2-like expression in order to avoid Fe toxicity.…”

Section: Resultssupporting

confidence: 85%

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Comparative analysis of iron deficiency chlorosis responses in soybean (Glycine max) and barrel medic (Medicago truncatula)

Santos¹,

Serrão²,

Vasconcelos³

2016

Rev. Ciênc. Agr.

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A B S T R A C TLegume grains have an important socio-economical role, being highly utilized in human and animal nutrition. Although iron (Fe) is abundant in the earth's crust, its limited solubility makes it poorly bioavailable for plants, contributing to iron deficiency chlorosis (IDC). In this work the physiological and molecular mechanisms associated with IDC were studied, namely, the mechanisms involved on Fe deficiency response, as well as a new Fe metabolism related gene in two important legume crops, Glycine max and Medicago truncatula. Fe deficient plants developed: decreased root and shoot length, increased number of secondary roots and lower chlorophyll levels. Fe shoot content decreased six-and 11-fold for G. max and M truncatula in Fe-deficiency. Whilst in G. max roots no significant differences were detected, in M. truncatula roots Fe decreased nine-fold in Fe-deficiency. Genes involved in Fe uptake (FRO2-like and IRT1-like), were over-expressed in roots of Fe-sufficient G. max and in Fe-deficient M. truncatula. VIT1-like, YSL1-like and ferritin presented higher expression levels in Fe-sufficient shoots and roots, whereas NRAMP3-like and GCN2-like showed higher expression values in Fe-deficiency.

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Section: Resultssupporting

confidence: 88%

Section: Resultssupporting

confidence: 85%

Comparative analysis of iron deficiency chlorosis responses in soybean (Glycine max) and barrel medic (Medicago truncatula)

Santos¹,

Serrão²,

Vasconcelos³

2016

Rev. Ciênc. Agr.

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“…This family of ligands was considered as suitable to formulate new Fe fertilizers to address IDC in the sequence of our previous study in which a [Fe(3,4‐HPO) 3 ] complex, [Fe(mpp) 3 ], showed high efficacy on Fe chlorosis prevention in soybean ( Glycine max L.) plants when compared to the commercial Fe chelate [FeEDDHA] (Santos, Carvalho et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A combined physiological and biophysical approach to understand the ligand‐dependent efficiency of 3‐hydroxy‐4‐pyridinone Fe‐chelates

et al. 2020

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Ligands of the 3‐hydroxy‐4‐pyridinone (3,4‐HPO) class were considered eligible to formulate new Fe fertilizers for Iron Deficiency Chlorosis (IDC). Soybean (Glycine max L.) plants grown in hydroponic conditions and supplemented with Fe‐chelate [Fe(mpp)3] were significantly greener, had increased biomass, and were able to translocate more iron from the roots to the shoots than those supplemented with an equal amount of the commercially available chelate [FeEDDHA]. To understand the influence of the structure of 3,4‐HPO ligand on the role of the Fe‐chelate to improve Fe‐uptake, we investigated and report here the effect of Fe‐chelates ([Fe(mpp)3], [Fe(dmpp)3], and [Fe(etpp)3]) in addressing IDC. Chlorosis development was assessed by measurement of morphological parameters, quantification of chlorophyll and Fe, and other micronutrient contents, as well as measurement of enzymatic activity (FCR) and gene expression (FRO2, IRT1, and Ferritin). All [Fe(3,4‐HPO)3] chelates were able to provide Fe to plants and prevent IDC but with a different efficiency depending on the ligand. We hypothesize that this may be related with the distinct physicochemical characteristics of ligands and complexes, namely, the diverse hydrophilic–lipophilic balance of the three chelates. To test the hypothesis, we performed an EPR biophysical study using liposomes prepared from a soybean (Glycine3 max L.) lipid extract and spin probes. The results showed that the most effective chelate [Fe(mpp)3] shows a preferential location close to the surface while the others prefer the hydrophobic region inside the bilayer. Significance statement The 3‐hydroxy‐4‐pyridinone Fe‐chelates, [Fe(mpp)3], [Fe(dmpp)3], and [Fe(etpp)3], were all able to provide Fe to plants and prevent IDC. Efficacy is dependent on the structure of the ligand. From an EPR biophysical study using spin probes and liposomes, prepared from a soybean lipid extract, we hypothesize that this may be related with the distinct preferential location close to the surface or on the hydrophobic region of the lipid bilayer. [Fe(mpp)3] provide higher amounts of Fe in the leaves.

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“…al., 2014). Since soybean is a dicot plant which belongs to strategy I for iron absorption, usually it exhibited iron deficiency induced chlorosis on calcareous soils (Santos et. al., 2016).…”

Section: The P and Fe-efficient Soybean 03-3 Would Be A Valuable Varimentioning

confidence: 99%

Effects of Fe-deficient conditions on Fe uptake and utilization in P-efficient soybean

Qiu

Dai

Wang

et al. 2017

Plant Physiology and Biochemistry

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Phosphorus (P)-efficient soybean (Glycine max) plants absorb and utilize P with high efficiency. To investigate the effects of iron (Fe)-deficient conditions on the absorption and utilization of Fe in P-efficient soybean plants, two soybean cultivars with different P efficiency, the 03-3 (P-efficient variety) and Bd-2 (P-inefficient variety), were used in this study. The two soybean cultivars were grown in nutrient solution containing Fe concentrations of 0 (Fe0), 20 (Fe20), 40 (Fe40), or 80 (Fe80) μM for 7 days. The Fe reductase activity of roots was higher in 03-3 plants grown under the Fe0, Fe20, and Fe40 treatments than in Bd-2 plants and the total Fe uptake was greater in 03-3 plants under the Fe40 treatment. GmFRD3a was much more highly expressed in the stem of 03-3 than in that of Bd-2, and significantly more iron was transported to 03-3 plant shoots during Fe0 treatment. Chlorosis in young leaves caused by Fe deficiency under the Fe0 and Fe20 treatments was alleviated by increased Fe concentration in shoots. Increased levels of active Fe in young 03-3 leaves under Fe-deprivation conditions (Fe0) and maintenance of stable Fe concentrations in 03-3 shoots subjected to Fe20, Fe40, and Fe80 treatments suggested that the P-efficient 03-3 cultivar is also Fe-efficient. It is suggested that 03-3 soybean cultivar should be a good resource for application to farm field.

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Effect of tris(3-hydroxy-4-pyridinonate) iron(III) complexes on iron uptake and storage in soybean (Glycine max L.)

Cited by 28 publications

References 77 publications

Comparative analysis of iron deficiency chlorosis responses in soybean (Glycine max) and barrel medic (Medicago truncatula)

Comparative analysis of iron deficiency chlorosis responses in soybean (Glycine max) and barrel medic (Medicago truncatula)

A combined physiological and biophysical approach to understand the ligand‐dependent efficiency of 3‐hydroxy‐4‐pyridinone Fe‐chelates

Effects of Fe-deficient conditions on Fe uptake and utilization in P-efficient soybean

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