Effect of Triparanol on Synthesis of Squalene and Tetrahymanol by Tetrahymena pyriformis

Shorb, Mary S.; Dunlap, Brian; Pollard, William O.

doi:10.3181/00379727-118-30065

Cited by 11 publications

(6 citation statements)

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“…It is important, therefore, that certain hypocliolesteremic drugs inhibit the multiplication of microorganisms which represent both cellular groups. The growth inhibition by hypocholesteremic drugs of certain bacteria, algae, fungi, and ascomycetes has previously been reported (34)(35)(36)(37)(38). The precedence for showing the inhibition of growth of microorganisms by both nonsteroidal (14 and references therein) and azasteroid hypocholesteremic drugs (13,38,39) has been established.…”

Section: Discussionmentioning

confidence: 93%

Structure‐function activity of azasterols and nitrogen‐containing steroids

Kabara¹,

Holzschu²,

Catsoulacos³

1976

Lipids

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Thirty-nine nitrogen-containing steroids were tested against two gram-negative, five gram-positive, and two yeast organisms. Many of these steroids have been previously reported to inhibit various metabolic processes involving sterol metabolism. While low minimal inhibitory concentration (MIC) values were recorded for sterol producing yeast, growth of bacteria which contain no sterols was also inhibited. Structure-function studies provided no relationship between biological activity and hypocholesteremic effects of these azasteroids. A hypothesis put forward is that amino and azasteroids are effectors of membrane which, in the case of mitochondria, lead to changes in adenosine triphosphate levels and/or dehydrogenase activity. Their effects on sterol metabolism, therefore, may be of secondary consideration.

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Section: Discussionmentioning

confidence: 93%

Structure‐function activity of azasterols and nitrogen‐containing steroids

Kabara¹,

Holzschu²,

Catsoulacos³

1976

Lipids

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“…Conversely, inhibitors of oxidative phosphorylation have been found to inhibit the biosynthesis of non-saponifiable material and cholesterol in rat liver homogenates ( 12). Triparanol is a well known inhibitor of cholesterol synthesis in mammals (6) and fatty acid ( 7 ) and triterpenoid alcohol (tetrahymenol) synthesis (8) in the ciliate Tetrahymena. Triparanol is an active inhibitor of aerobic respiration in Ochromonas and inhibits multiplication and fatty acid synthesis as well.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Ochromonas Respiration by Hypocholesteremic Compounds and its Annulment by Unsaturated Fatty Acids

Aaronson

Roze

Keane

et al. 1969

The Journal of Protozoology

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SYNOPSIS. Eleven of 14 hypocholesteremic drugs inhibited aerobic respiration of the phytoflagellate Ochromonas danica. Lecithin and unsaturated fatty acids annulled the inhibition of respiration by triparanol. Saturated fatty acids, squalene, cholesterol and a mixture of amino acids, purines, and water‐soluble vitamins were inactive. Unsaturated fatty acids only annul the triparanol inhibition if preincubated or added simultaneously with triparanol. Evidence is presented suggesting that triparanol and oleic acid form a complex or micelle; presumably in this form the triparanol no longer inhibits aerobic respiration.

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“…An absolute sterol nutritional requirement can be induced in Tetliahymena pyrijormis by a number of growth inhibitors including compounds known to block cholesterol biosynthesis (2,24,25). Shorb, Dunlap and Pollard (35) found that triparanol blocks the synthesis of tetrahymanol with the resultant accumulation of squalene.…”

mentioning

confidence: 99%

Cholesterol Inhibition of Pentacyclic Triterpenoid Biosynthesis in *Tetrahymena pyriformis**†

Conner

Landrey

Burns

et al. 1968

The Journal of Protozoology

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SYNOPSIS. Tetrahymena pyriformis synthesizes tetrahymanol and “diplopterol” from acetate, mevalonate or squalene. The formation of these pentacyclic triterpenoid alcohols is inhibited by the addition of cholesterol to the culture fluid of the ciliates. Cholesterol also inhibits the biosynthesis of squalene from acetate or mevalonic acid. The synthesis of other terpene derivatives from acetate and mevalonate continues in the presence of cholesterol, thus suggesting that a major block occurs after “isoprene” formation and before squalene formation. Further, inhibition of squalene conversion to the pentacyclic alcohols by cholesterol has been established.

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Effect of Triparanol on Synthesis of Squalene and Tetrahymanol by Tetrahymena pyriformis

Cited by 11 publications

References 1 publication

Structure‐function activity of azasterols and nitrogen‐containing steroids

Structure‐function activity of azasterols and nitrogen‐containing steroids

Inhibition of Ochromonas Respiration by Hypocholesteremic Compounds and its Annulment by Unsaturated Fatty Acids

Cholesterol Inhibition of Pentacyclic Triterpenoid Biosynthesis in *Tetrahymena pyriformis**†

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Effect of Triparanol on Synthesis of Squalene and Tetrahymanol by Tetrahymena pyriformis

Cited by 11 publications

References 1 publication

Structure‐function activity of azasterols and nitrogen‐containing steroids

Structure‐function activity of azasterols and nitrogen‐containing steroids

Inhibition of Ochromonas Respiration by Hypocholesteremic Compounds and its Annulment by Unsaturated Fatty Acids

Cholesterol Inhibition of Pentacyclic Triterpenoid Biosynthesis in Tetrahymena pyriformis*†

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Cholesterol Inhibition of Pentacyclic Triterpenoid Biosynthesis in *Tetrahymena pyriformis**†