Effect of Trigonella Foenum-Graecum Foenugreek oil extract on some parameters of sperms and in vitro fertilization in mic

Abstract

This project, aims to study the effect of trigonella seeds oil extract on body weight , testes weight and some parameter of reproductive system in male mice , and on In vitro fertilization (IVF) , cleavage stages of embryos in females. Fifteen adult (8-9 weeks) males and females mice were divided into three groups , each group containing 5 mice , first and second groups were treated with (2 and 4 mg/kg body weight) of Trigonella oil extract (intraperitonally) while the third group treated with normal saline as control group . After 35 days of treatment with Trigonella the animals (male and female) were sacrificed by dislocation of cervical vertebrate. Isolated sperms from tail of epidydymis were used for the following tests. Sperm motility, dead / live sperm, sperm abnormalities and serum ws collected to assay testosterone. Isolatation of ova from oviduct after micing in 500μl of TCM-199 media serum blood to assay FSH and LH . Sperm and matured ova were incubated in TCM-199 media for insemination. The results showed increase in body weight and testes weight in groups treated with trigonella. significant increase in sperm activity and decrease in percentage of dead sperm and abnormalities in groups treated with trigonella oil. The male testosterone and female FSH, LH increased significantly in groups treated with trigonella. IVF study showed significant increase in percentage of maturation, fertilization and cleavage (2-4 cells).