Effect of Trichoderma koningiopsis on Chickpea Rhizosphere Activities under Different Fertilization Regimes

Abstract

Interaction of plant growth promoting microorganisms (PGPMs) with plants involves interplay at physical, physiological and molecular levels. Proliferation and root colonization of PGPMs manipulate the rhizosphere to optimize plant functions. This benefits plant by nutrient enrichment and induction of plant vigor and defense system. The present work aims to decipher the rhizosphere modulations promoted under different fertilization regimes by an organic acid producing Trichoderma koningiopsis strain (NBRI-PR5). Chickpea was selected as the host plant for the study since it responds well to the application of in/organic fertilizers and PGPMs. Microbial communities associated with the rhizosphere were studied by determining culturable population of heterogeneous microflora, and rhizosphere functions were studied by determining the soil enzyme activities and HPLC profiles of organic acids in root exudates. Application of NBRI-PR5 induced changes in rhizosphere in consent with the amendments. The changes observed in microbial populations were found to be associated with the rhizosphere enzymes. The inhibitory effect of chemical fertilizers on rhizosphere microflora was evident from least bacterial CFU observed in the NPK treatments. No detection of alkaline phosphatase enzyme in all the treatments with NBRI-PR5, with organic or inorganic amendments evidently represents the acidified rhizosphere. Similarly, an opposite trend in DHA and protease enzyme activities in the rhizosphere of FYM and FYM+PR5 treated plants showed that NBRI-PR5 had reframed microbial activities to facilitate nutrient uptake in plants rather than fix in the microbes. It is concluded from the study that NBRI-PR5 fatefully modulates rhizosphere activities, specific to different fertilization regimes by varying the enzyme activities to maximize the utilization of available nutrients.

Compost fortification with lignocellulolytic fungi for wheat cultivation using fewer mineral fertilizers amount in sandy soil

“…Alkaline phosphatase, but not acid phosphatase, is mainly associated with microorganisms, so an increase in microbial biomass conferred the observed higher alkaline phosphatase activity. The enhanced decomposition of organic matter, especially with the action of inoculated fungi, appeared a regulatory influence on the alkaline phosphatase activity matching the findings of Tandon et al (2018) for microbial activities in rhizosphere of chickpea inoculated with Trichoderma koningiopsis under dissimilar fertilization treatments.…”

“…They suggested that inoculated organic fertilizer with Trichoderma is a better option for maintaining a relatively sustainable microbiome in mono-cropped soils under a 25% reduction of inorganic fertilizer. Also, Tandon et al (2018) attributed the inhibitory effect of 100%-NPK chemical fertilizers comparing 50%-NPK on bacterial CFU of microflora in chickpea rhizosphere to lowering soil pH and exert destructive effects caused by mineral fertilizers.…”

Effects of biocontrol agents and compost against the Phytophthora capsici of zucchini and their impact on the rhizosphere microbiota

“…phosphate -Tandon et al, 2018) and is more efficient and competitive that many other fungi. In consequence, it can therefore be used effectively to improve the soil structure and promote the establishment and maintenance of other beneficial microorganisms (Benitez et al, 2004).…”

Role of Trichoderma aggressivum f. europaeum as Plant-Growth Promoter in Horticulture

“…Diánez et al [16] described a highly similar IAA production for T. saturnisporum; therefore, the in vitro production capacity of IAA was preserved despite maintaining the isolate in the laboratory for 10 years. Similarly, phosphate solubilisation by Trichoderma species has been described both in vitro and in vivo [52][53][54]. Recently, Tandon et al [55] evaluated P solubilisation of different Trichoderma koningiopsis isolates under abiotic stress conditions and determined a range from 1.6 to 71 µg mL −1 .…”