Effect of tributyltin chloride (TBT-Cl) exposure on expression ofHSP90β1in the river pufferfish (Takifugu obscurus): evidences for its immunologic function involving in exploring process

Abstract

14HSP90β1 (known as GP96) is a vital endoplasmic reticulum depended chaperonin 15 among the HSPs family. It plays important roles in regulating the growth, 16 development, differentiation, and apoptosis of cells. Furthermore, it always processes 17 and presents antigen of the tumor and keeps balance for the intracellular environment. 18In the present study, we explored the effect of tributyltin chloride (TBT-Cl) exposure 19 on HSP90β1 expression in river pufferfish, Takifugu obscurus. The full length of 20 To-HSP90β1 was gained with 2775 bp in length, an ORF obtained with 2412 bp 21 encoding an 803 aa polypeptide. The phylogenetic tree was constructed and showed 22 the close relationship to other fish species. The HSP90β1 mRNA transcript was 23 expressed in all tissues investigated. After the acute and chronic exposure of TBT-Cl, 24 the mRNA level of To-HSP90β1 significantly up-regulated in tissues of liver and gill. 25 Moreover, the histochemistry study indicated the injury degree of TBT-Cl on liver and 26 gill. Immunohistochemistry (IHC) staining results implied the cytoplasm 27 reorganization after TBT-Cl stress and the function of immunoregulation for 28 To-HSP90β1. All the results indicated that HSP90β1 may involve in the resistance to 29 the invasion of TBT-Cl for keeping autoimmune homeostasis.30