Effect of Trehalose on the Functional Properties of Photosystem II

Yanykin, Denis V.; Khorobrykh, A. A.; Semenov, Alexey Yu.; Mamedov, Mahir D.

doi:10.1007/978-3-030-67407-6_17

Cited by 2 publications

(2 citation statements)

References 114 publications

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“…It is suggested that trehalose may change protein packing to the more optimal conformation and/or cause protein-protein interactions in the WOC, which increase the rate of turnover of S-state transitions and, consequently, the efficiency of water photooxidation and oxygen evolution [99]. It seems that such changes induced by trehalose also may promote the efficiency of Mn 4 CaO 5 cluster photoassembly.…”

Section: Discussionmentioning

confidence: 99%

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Yanykin,

Kazantseva,

Khorobrykh

2023

Horticulturae

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The effect of osmolytes (trehalose, sucrose, betaine, D-glucose and hydroxyectoine) on the photoassembly of the functionally active inorganic core of the water-oxidizing complex (Mn4CaO5 cluster) in Mn-depleted PSII preparations (apo-WOC-PSII) was investigated. It was revealed that the efficiency of the photoassembly of the Mn4CaO5 cluster was considerably (three times) increased in the presence of 1 M disaccharides (trehalose or sucrose) in contrast to other osmolytes. It was found that the osmolytes (especially trehalose or sucrose) improved the redox interaction of exogenous Mn2+ with apo-WOC-PSII, enhanced the protective effect of Mn2+ against the photoinhibition of apo-WOC-PSII, protected CaCl2-treated PSII preparations against thermoinactivation, and stabilized the water-oxidizing complex and electron transport from QA to QB in native PSII preparations during heat treatment. It is suggested that the ability of osmolytes to enhance the efficiency of the photoassembly of a Mn4CaO5 cluster depends on their effect on the following key processes: the redox interaction of Mn2+ with apo-WOC-PSII; the stability of apo-WOC-PSII to photoinhibition during the photoactivation procedure; and the stabilization of both the newly assembled functionally active Mn4CaO5 cluster and the electron transfer from QA to QB.

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Section: Discussionmentioning

confidence: 99%

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Yanykin,

Kazantseva,

Khorobrykh

2023

Horticulturae

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“…The processing temperature melts the trehalose sugar, which functions as a binder to effectively make the mixture consolidation. Some researchers [ 11 , 18 , 19 ] have revealed that the recommended processing temperature of trehalose sugar ranges from 120 to 190 °C. Tests were implemented by using Taguchi’s L 9 (3 4 ) orthogonal array.…”

Section: Water-soluble Core Developmentmentioning

confidence: 99%

A Water-Soluble Core for Manufacturing Hollow Injection-Molded Products

Lin

Yang

2022

Polymers

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To manufacture a complicated hollow product without any assembly process, for example, the plastic intake manifold, is difficult by the traditional injection molding method. The fusible-core technique, which uses a low-melting-point alloy as a sacrificial core, was developed to solve this problem; however, the limited selection of resin type and the huge capital investment have caused this technique to spread slowly. In this work, a novel method is established that can produce similar products without the limitation of resin type, as well as a lower-energy-consumption process. The concept of the enveloped core defined by a water-soluble core assembled with a shell is proposed herein; it provides both rigidity and toughness to resist the pressure during the injection molding process. The shape of the enveloped core equals the internal contour of the designated product. An insert molding process was introduced to cover the enveloped core with a skin layer. Cut out the end of the enveloped core and immerse it into a water bath. When the water-soluble core inside the shell is dissolved, the product with a special internal contour is accomplished. A tee-joint is presented to demonstrate how the proposed method can be utilized. The optimal ingredient of the core and processing parameters are determined by the Taguchi method. The result shows that the proposed product is molded successfully when the compressive strength of the core is larger than 2 MPa. In addition, the eccentricity measurement of internal contour of the optimal sample exhibits a 56% improvement, and the required time for the core removal is less than 154 s.

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Effect of Trehalose on the Functional Properties of Photosystem II

Cited by 2 publications

References 114 publications

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

Effect of Osmolytes on Photoassembly of Functionally Active Mn4CaO5 Cluster in Mn-Depleted Photosystem II Preparations Isolated from Spinach Leaves

A Water-Soluble Core for Manufacturing Hollow Injection-Molded Products

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