Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma

Lindena, J.; Küpper, Werner; Trautschold, I.

doi:10.1515/cclm.1982.20.2.95

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…Blood CK activity does not depend on the site of sampling (Henry et al, 1970;Lindena et al, 1982a), but an incorrect venepuncture can result in higher activities through puncture of underlying muscles (Fayolle et al, 1992). Identical CK activity was reported in serum and heparinized plasma (Cairns et al, 1978), but we found higher activities in serum, probably owing to leakage of CK from platelets (Auguste, 1992).…”

Section: Reference Values and Physiological Factors Of Variationsupporting

confidence: 54%

“…It also depends on the type of muscle: CK activity is more elevated in fast-contracting (e.g.m. sartorius) than in slow-contracting (m. gastrocnernius) muscles (Lindena et al, 1982a). There are no sex-related differences (Clampitt and Hart, 1978).…”

Section: Distribution In the Organs Of The Dogmentioning

confidence: 97%

“…After its release from muscle, CK reaches the plasma mostly via the lymphatic route (Bolter and Critz, 1976a,b;Lindena et al, 1979Lindena et al, , 1982aLindena et al, ,b, 1986b. CK in the lymph may be inactivated by action on its thiol groups, while that in the blood would be mainly captured by the liver (Clark et al, 1978;Carlson et al, 1982).…”

Section: Catalytic Activitymentioning

confidence: 99%

“…Its activity in the thoracic canal is relatively low, similar to or moderately higher than in plasma (Bolter and Critz, 1976a,b;Friedel et al, 1976;Keyl et al, 1980Keyl et al, , 1981Lindena et al, 1986c). Lymph and CK flow are reduced in anaesthetized animals, in which passive mobilization of the limbs produces an increase in CK activity (Lindena et aL, 1982a(Lindena et aL, ,b, 1984. CK concentration and CK flow in the thoracic duct increase during physical exercise (Bolter and Critz, 1974;Lindena et al, 1984Lindena et al, , 1986b.…”

Section: Isoenzyme Patternmentioning

confidence: 99%

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Creatine kinase in the dog: A review

et al. 1993

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In the dog, creatine kinase (CK) is mostly present in the skeletal muscles, myocardium, brain and intestine. The MM isoenzyme predominates in muscles and myocardium. In plasma, reference values depend on the technique used and CK-MB accounts for about 30-45% of total CK activity. Sex has no influence on plasma CK activity, which is higher in young dogs than in adults. Plasma CK is elevated after physical exercise. After its release from the cells, CK reaches the plasma mostly via the lymphatic route and then remains in the plasma compartment. It is rapidly cleared with a half-life of about 2 hours. Muscle diseases are the main source of plasma CK elevations: inherited myopathies, malignant hyperthermia, hypothyroidism, vitamin E-selenium deficiency, prolonged decubitus, intramuscular injections, surgery, etc. Plasma CK is also increased in experimental myocardial infarction, for which the dog is an interesting model, allowing quantification of the damage by measuring the total CK activity released.

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Section: Reference Values and Physiological Factors Of Variationsupporting

confidence: 54%

Section: Distribution In the Organs Of The Dogmentioning

confidence: 97%

Section: Catalytic Activitymentioning

confidence: 99%

Section: Isoenzyme Patternmentioning

confidence: 99%

See 2 more Smart Citations

Creatine kinase in the dog: A review

et al. 1993

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“…The second hypothesis is compatible with metabolic impairment of skeletal muscles during fetal hypoxia resulting in CK release to lymphatic space. LINDENA et al [14] demonstrated six times higher CK activity in lymph as compared to serum. Lymph flow is related to muscle activity.…”

Section: Discussionmentioning

confidence: 95%

Cord blood serum creatine kinase isoenzymes with placental dysfunction

Niklinski¹,

Palynyczko²,

Jóźwik³

et al. 1987

Journal of Perinatal Medicine

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Enzyme activities in thoracic duct lymph and plasma of anaesthetized, conscious resting and exercising dogs

Lindena

Küpper

Trautschold

1984

Europ. J. Appl. Physiol.

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The significance of changes in lymph flow for the extracellular distribution and transport of cellular enzymes and for the level of enzyme activities in plasma was investigated. Specimens of thoracic duct lymph were obtained from an extracorporal lymph shunt in anaesthetized, conscious resting and treadmill exercising dogs (6 km X h-1 for 1 h) The activity of 10 enzymes and of protein content in lymph and plasma were studied, as well as lymph flow, lymphatic transport, and the lymph-plasma ratio of these compounds. Lactate, pH, and blood gases were monitored in venous blood. Lymph flow of 0.80 ml X min-1 in anaesthetized dogs more than doubled (to 1.86 ml X min-1) when the animals were conscious and resting. In anaesthetized dogs lymph enzyme activity was higher only for enzymes of predominately hepatic origin, such as choline esterase (CHE) and alanine aminoferase (ALAT), and was lower for aspartate aminotransferase (ASAT) and aldolase (ALD). In conscious dogs, due to activation of the skeletal muscle "tissue pump", lymphatic transport of enzymes with rather high activity in skeletal muscle, and of protein, is significantly enhanced. Enzyme activities in plasma, however, did not differ between the groups. Lymph-plasma activity ratios higher than one were found for lactate dehydrogenase (LDH), malate dehydrogenase (MDH), ASAT, creatine kinase (CK), ALD, and phosphohexose isomerase (PHI). Exercise stimulated lymph flow up to 4.9 ml X min-1, and increased the lymphatic activities of those enzymes with a lymph-plasma ratio higher than unity, these enzymes increasing in the plasma due to the highly increased lymphatic transport.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effect of Transient Hypoxia in Skeletal Muscle on Enzyme Activities in Lymph and Plasma

Cited by 3 publications

References 12 publications

Creatine kinase in the dog: A review

Creatine kinase in the dog: A review

Cord blood serum creatine kinase isoenzymes with placental dysfunction

Enzyme activities in thoracic duct lymph and plasma of anaesthetized, conscious resting and exercising dogs

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