Effect of transforming growth factor β1 on chondrogenic differentiation of cultured equine mesenchymal stem cells

Worster, Allison A.; Nixon, Alan J.; Brower-Toland, Brent D.; Williams, Jeffrey A.

doi:10.2460/ajvr.2000.61.1003

Cited by 130 publications

(108 citation statements)

References 34 publications

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“…The exclusive development towards chondrogenesis was observed only upon administration of recombinant BMP2 in pellet cultures of human MSC [142]. The exposure of equine MSCs to TGFβ-1 resulted in higher collagen II expression in monolayer cultures [145], and was superior to the treatment with hyaluronic acid and synovial fluid for chondrogenesis in pellet cultures [146]. TGFβ-1 was also demonstrated to induce chondrogenesis in bovine MSCs in pellet culture [147].…”

Section: Vivomentioning

confidence: 90%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

210

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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Section: Vivomentioning

confidence: 90%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

210

156

View full text Add to dashboard Cite

show abstract

“…TGF-β1 acts via high-affinity interaction with a heteromeric receptor complex comprising two structurally-related serine-threonine kinases, the type I and II receptors, and transduces its signals through Smad proteins intracellularly (Miyazono 2000). In addition to the pellet and micromass culture systems, Worster et al (2000a) have induced chondrogenesis in equine MSCs in monolayer culture by using TGF-β1. We have reported the spontaneous chondrogenic differentiation of bovine MSCs, independent of exogenous bioactive substances, in a pellet culture system and thereby have confirmed the importance of culture conditions prior to chondrogenesis (Bosnakovski et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…To induce chondrogenic differentiation, the cells were treated with 1 ng/ml or 5 ng/ml transforming growth factor beta 1 (TGF-β1; R&D Systems, Minneapolis, Minn., USA) in plain medium (Worster et al 2000a(Worster et al , 2000b. In the control group, the cells were cultured in control medium alone.…”

Section: Chondrogenic Differentiation In Monolayer Culturementioning

confidence: 99%

“…The marrow was drawn into syringes containing heparin (1,000 units) and kept on ice until later use. Bovine MSC were isolated by previously described methods (Worster et al 2000a(Worster et al , 2000bMastrogiacomo et al 2001;Holzer et al 2002;Bosnakovski et al 2004). Briefly, the bone marrow sample was washed twice with phosphate-buffered saline (PBS) and twice with Dulbecco's Modified Eagle Medium (DMEM; GIBCO BRL, Grand Islands, N.Y., USA).…”

Section: Harvesting and Isolation Of Bovine Mscmentioning

confidence: 99%

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Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Bosnakovski¹,

et al. 2004

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The bone marrow harbors a population of mesenchymal stem cells (MSCs) that possess the potential to differentiate into bone, cartilage, and fat, and along other tissue pathways. To date, MSCs from various species have been studied. Despite the bovine experimental model being widely used in experiments in vivo and in vitro, only a limited amount of information regarding bovine MSCs is available. The aim of this study was to isolate and induce the multilineage mesenchymal differentiation of bovine MSCs, thereby initiating further research on these cells. Bovine MSCs were isolated from eight calves, and osteogenic, chondrogenic, and adipogenic differentiation was induced by using a combination of previously reported protocols for other species. The level of differentiation was evaluated by histological examination and by analyzing the expression of tissue-specific genes by a quantitative "real time" reverse transcription/polymerase chain reaction technique. Following osteoinduction, the isolated fibroblast-like cells transformed into cuboidal cells and formed alkaline-phosphatase-positive colonies; during differentiation, these colonies transformed into mineralized nodules. In addition, osteogenesis was followed by osteocalcin and collagen type I mRNA expression. Chondrogenesis was confirmed by the demonstration of collagen type II, aggrecan, and sox9 mRNA expression in the cells stimulated by transforming growth factor β1 in monolayer culture. After being cultured in an adipogenesis-inducing medium, the MSCs responded by the accumulation of lipid vacuoles and the expression of adipocyte-specific genes. We have therefore demonstrated that cells harvested from bovine bone marrow are capable of in vitro extensive multiplication and multilineage differentiation, making them a relevant and invaluable model in the field of stem cell research.

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“…Among the list of potentially useful cDNAs for cartilage repair are anabolic growth factors of the transforming growth factor (TGF)-b superfamily, [13][14][15] including TGF-bs 1-3, 16 several of the bone morphogenetic proteins (BMPs), [17][18][19] insulin-like growth factor (IGF)-1, 8,20,21 fibroblast growth factors (FGFs) 7,22 and epidermal growth factor (EGF). 23 Transcription factors such as Sox-9, 24-26 L-Sox 5 and Sox-6 25 that promote chondrogenesis or the maintenance of the chondrocyte phenotype present another class of biologics that may be useful.…”

Section: Candidate Cdnasmentioning

confidence: 99%