Effect of transfer of donor corneal tissue from McCarey–Kaufmann medium to Optisol-GS on corneal endothelium

Kapur, Neha; Gunda, Sridevi; Dixit, Surender; Chauhan, Lokesh; Acharya, Manisha; Mathur, Umang

doi:10.4103/ijo.ijo_677_17

Cited by 4 publications

(5 citation statements)

References 18 publications

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“…Further, the human cornea becomes thinner with age [19,20]. Our ECD, hexagonality, and CV results were similar to those reported by Gupta et al and Kapur et al [11,15].…”

Section: Discussionsupporting

confidence: 87%

“…The demographics of our study population were similar to several previous studies [13,14]. However, Kapur et al had a younger mean population age and a female majority of donors like Galgauskas et al [15,16]. In our study, all corneas were retrieved within six hours of death, complying with India's Joint Review of Eye Banking Standards [17].…”

Section: Discussionsupporting

confidence: 75%

“…Historically, MK medium is the first successful method for storing excised cornea (corneoscleral button) in a chemically defined tissue culture medium at 4°C for up to four days and has been reported to be efficient [11,15]. However, we preserved most donor tissues (77%) in Cornisol, an intermediate storage medium as effectual as Optisol-GS (Bausch & Lomb Inc., Laval, Canada) in preserving donor corneal tissues for 14 days [14].…”

Section: Discussionmentioning

confidence: 99%

“…However, we preserved most donor tissues (77%) in Cornisol, an intermediate storage medium as effectual as Optisol-GS (Bausch & Lomb Inc., Laval, Canada) in preserving donor corneal tissues for 14 days [14]. Optisol-GS is very effective in preserving ECD and hexagonality but is approximately 10 times the cost of conventional MK media [15]. Therefore, in low-income countries like India, eye banks continue to place corneal tissue in MK media initially, as it can keep tissue viable for up to four days.…”

Section: Discussionmentioning

confidence: 99%

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Qualitative and Quantitative Evaluation of Donor Corneal Tissue by Slit Lamp and Specular Microscopy

Patil¹,

Lune²,

Paranjape³

et al. 2022

Cureus

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BackgroundIn India, donor eye collection and promotion of eye banking are insufficient to meet the needs. By adequately evaluating donor corneas, eye banks can maximize the number of viable corneas for transplantation. This study evaluated donor corneal tissue based on age, lens status, and cause of death by their morphology and endothelial cell count via slit lamp and specular microscopy. MethodsWe conducted a prospective observational study of all eye bank donor corneas indicated for eye donation at a tertiary hospital and research center in Western Maharashtra between September 2019 to December 2021. We evaluated the corneoscleral discs by slit-lamp microscopy specular microscopy. We analyzed donor corneas quantitatively and qualitatively and graded them accordingly. We also collected blood samples for serological testing and the donor's behavioral and family medical histories. ResultsWe collected 94 eyes from 47 donors; the mean age of the donor population was 48.2 years, and most donors were aged 41 to 80 years. Thirty-one donors (65.96%) were male, and 16 were female (34.04%. For preservation, we used Cornisol (Aurolab, Madurai, India) in 36 cases (77%) and McCarey-Kaufman medium in 11 cases (23%). We found a mean endothelial cell density (ECD) of 2214.40/mm2, with hexagonality of 53.05%, and a coefficient of variation of 38.01. Further, we observed that ECD and hexagonality of cells in phakic donors were significantly greater than that of pseudophakic (PP) donors. Moreover, ECD and hexagonality significantly decreased in donors with the chronic disease compared to those who had a sudden, unexpected death. ConclusionCorneal grafts from younger donors, phakic donors, and donors who experienced an acute cause of death were qualitatively and quantitatively significantly better than those of older donors, PP donors, and donors who experienced sudden, unexpected death. Therefore, eye bank specular examination can improve tissue utilization and transplantation success. Therefore, we strongly recommend that eye bank personnel evaluate their donor tissue with a specular microscope to enhance the quality of eye care.

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“…Further, the human cornea becomes thinner with age [19,20]. Our ECD, hexagonality, and CV results were similar to those reported by Gupta et al and Kapur et al [11,15].…”

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Qualitative and Quantitative Evaluation of Donor Corneal Tissue by Slit Lamp and Specular Microscopy

Patil¹,

Lune²,

Paranjape³

et al. 2022

Cureus

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“…There is a natural loss of endothelial cells in hypothermic corneal storage media, ranging from 3-9% at 7 days and 15-20% at 14 days according to various studies [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Endothelial cell viability comparing Eusol-C vs Optisol-GS as corneal preservation medium

Mena-Linares,

Vázquez-Maya,

García-Carmona

et al. 2023

HGMX

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Introduction:Optisol-GS is the most widely used corneal preservation medium worldwide, there is little evidence to compare it against Eusol-C as a corneal preservation medium. Objectives: To evaluate the viability of endothelial cells between two different corneal preservation media. Materials and Methods: It is a prospective, longitudinal, and comparative study, which was carried out in the eye bank of Hospital General de México "Dr. Eduardo Liceaga", from where the corneas of suitable donors were collected, the right cornea was preserved in Optisol-GS and the left in Eusol-C. Two evaluations were carried out on day 1 and 5. Endothelial cell density, coefficient of cell variation and hexagonality were analysed. Results: The decrease in endothelial cells on day 5 after collection of the corneoscleral tissue was 8.149% in the corneas stored in Optisol-GS and 7.614% in Eusol-C p=0.006. Cell variation increased 1.634% in . Conclusions: No superiority was found when comparing these two media, which shows that Eusol-C is as effective as Optisol-GS for corneal preservation for at least five days.

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A new storage medium containing amphotericin B versus Optisol-GS for preservation of human donor corneas

Mistò

Giurgola²,

Pateri

et al. 2020

Br J Ophthalmol

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Background/AimWe compared the quality of human donor corneas stored in a cold storage medium containing 2.5 μg/ml of amphotericin B (Kerasave, AL.CHI.MI.A. S.R.L., Ponte San Nicolò, Italy) and Optisol-GS (Bausch & Lomb Inc., Bridgewater, NJ, USA) for 14 days.MethodsSixteen pairs of human donor corneas were collected in Eusol-C (AL.CHI.MI.A. S.R.L., Ponte San Nicolò, Italy). Next, all tissues underwent the first evaluation that included the assessments of central corneal thickness (CCT), endothelial cell density (ECD) measured using both trypan blue staining and specular microscopy, endothelial cell (EC) mortality and morphology, and corneal transparency within 24 hours from recovery (Day 1). Afterwards, one cornea of each pair was transferred into Kerasave or Optisol-GS. ECD and CCT were also assessed at Day 7, and all the metrics were evaluated again at the end of the storage period (Day 14).ResultsAt all tested time points, no differences were found in the qualitative (corneal transparency, EC morphology) and quantitative metrics (ECD, CCT, EC mortality) between the Kerasave and the Optisol-GS storage groups. At Day 14, the corneas stored in Kerasave and Optisol-GS showed ECD of 2312±98 and 2335±128 cells/mm2 (p=0.886), CCT of 717±17 and 697±19 μm (p=0.454) and central EC mortality of 0.54%±0.40% and 0.14%±0.14% (p=0.719), respectively.ConclusionsThe new amphotericin B−containing medium Kerasave was comparable to Optisol-GS in terms of preservation of corneal characteristics at 2–8°C for 14 days.

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Effect of transfer of donor corneal tissue from McCarey–Kaufmann medium to Optisol-GS on corneal endothelium

Cited by 4 publications

References 18 publications

Qualitative and Quantitative Evaluation of Donor Corneal Tissue by Slit Lamp and Specular Microscopy

Qualitative and Quantitative Evaluation of Donor Corneal Tissue by Slit Lamp and Specular Microscopy

Endothelial cell viability comparing Eusol-C vs Optisol-GS as corneal preservation medium

A new storage medium containing amphotericin B versus Optisol-GS for preservation of human donor corneas

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