Effect of trans-resveratrol on signal transduction pathways involved in paclitaxel-induced apoptosis in human neuroblastoma SH-SY5Y cells

Nicolini, Gabriella; Rigolio, R; Scuteri, A; Miloso, Mariarosaria; Saccomanno, Domenica; Cavaletti, Guido; Tredici, G

doi:10.1016/s0197-0186(02)00132-8

Cited by 51 publications

(38 citation statements)

References 77 publications

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“…Cell lysates and immunoblotting analysis hMSCs were washed twice with ice-cold PBS and total cellular extracts were prepared as previously described [11]. To obtain nuclear protein extracts, the protocol described by Ronca et al was performed [12].…”

Section: Immunofluorescence Experimentsmentioning

confidence: 99%

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Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation

Foudah

Redondo

Caldara

et al. 2013

Cellular and Molecular Biology Letters

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Mesenchymal stem cells (MSCs) are multipotent cells that are able to differentiate into mesodermal lineages (osteogenic, adipogenic, chondrogenic), but also towards non-mesodermal derivatives (e.g. neural cells). Recent in vitro studies revealed that, in the absence of any kind of differentiation stimuli, undifferentiated MSCs express neural differentiation markers, but the literature data do not all concur. Considering their promising therapeutic potential for neurodegenerative diseases, it is very important to expand our knowledge about this particular biological property of MSCs. In this study, we confirmed the spontaneous expression of neural markers (neuronal, glial and progenitor markers) by undifferentiated human MSCs (hMSCs) and in particular, we demonstrated that the neuronal markers III-tubulin and NeuN are expressed by a very high percentage of hMSCs, regardless of the number of culture passages and the culture conditions. Moreover, the neuronal markers III-tubulin and NeuN are still expressed by hMSCs after in vitro osteogenic and adipogenic differentiation. On the other hand, chondrogenically differentiated hMSCs are negative for these markers. Our findings suggest that the expression of neuronal markers could be common to a wide range of cellular types and not exclusive for neuronal lineages. Therefore, the expression of neuronal markers alone is not sufficient to demonstrate the differentiation of MSCs towards the neuronal phenotype. Functional properties analysis is also required.

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Section: Immunofluorescence Experimentsmentioning

confidence: 99%

“…The immunoreactive proteins were visualized using an ECL chemiluminescence system (Amersham, Arlington Heights, IL, USA). DRG neuron and glial cell total protein extracts were prepared as previously described [11].…”

Section: Immunofluorescence Experimentsmentioning

confidence: 99%

Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation

Foudah

Redondo

Caldara

et al. 2013

Cellular and Molecular Biology Letters

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“…We used 1 µM resveratrol since a similar resveratrol concentration is reached in the plasma of rats after oral administration of red wine [38]. The 50 µM concentration was not cytotoxic in a neuroblastoma cell line [39] and almost completely inhibited lymphocyte cell proliferation [40].…”

Section: Discussionmentioning

confidence: 99%

Effect of resveratrol on matrix metalloproteinase-2 (MMP-2) and Secreted Protein Acidic and Rich in Cysteine (SPARC) on human cultured glioblastoma cells

Gagliano

Moscheni

Torri

et al. 2005

Biomedicine & Pharmacotherapy

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“…This mechanism explains the effect of paclitaxel on tumor cells and also on dividing neuronal-like cells such as human neuroblastoma SH-SY5Y cells but not on postmitotic neurons. Nevertheless, in post-mitotic neurons, paclitaxel may alter the neuronal transport by acting on microtubuli (2) and it may also modulate proteins involved in the intracellular transduction pathways (15,16). All these mechanisms may be important in explaining the effect of paclitaxel as an anticancer and as a neurotoxic drug (17,18).…”

Section: Introductionmentioning

confidence: 99%

“…This cell line is a reliable model for studying the neurotoxic effect of platinum compounds or taxanes (20) and for elucidating the mechanisms of diabetic neuropathy (21). Neurotoxicity was evaluated by the capacity of these compounds to induce apoptosis (10,15,22). We have previously reported that CDDP may protect human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis, whereas low doses of paclitaxel do not protect from CDDP neurotoxicity (23).…”

Section: Introductionmentioning

confidence: 99%

Low-dose cisplatin protects human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis

Villa¹,

Miloso²,

Nicolini³

et al. 2005

Molecular Cancer Therapeutics

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Combined anticancer therapy using platinum compounds and antitubulins has increased the risk of neurotoxicity. However, the combination of low-dose cisplatin (CDDP) with toxic doses of paclitaxel significantly reduces cellular death in a human neuroblastoma SH-SY5Y cell line. To analyze the mechanisms of this protection, we evaluated various signaling molecules possibly involved in apoptosis and some relevant cell cycle regulatory proteins. CDDP does not interfere with the tubulin-stabilizing action of paclitaxel. The evaluation of molecular pathways involved in apoptosis indicates that the Bcl-2 but not the caspases may be involved in the CDDP protection of paclitaxelinduced apoptosis. The increase in p53 protein and its nuclear accumulation suggests a possible involvement of p53 in CDDP protection. The use of the chemical inhibitor of p53, pifithrin A, excluded this possibility. The study of cyclins and the flow cytometric analysis (fluorescenceactivated cell sorting) suggest that CDDP exerts a protective action by blocking cells early in the cell cycle.

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Effect of trans-resveratrol on signal transduction pathways involved in paclitaxel-induced apoptosis in human neuroblastoma SH-SY5Y cells

Cited by 51 publications

References 77 publications

Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation

Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation

Effect of resveratrol on matrix metalloproteinase-2 (MMP-2) and Secreted Protein Acidic and Rich in Cysteine (SPARC) on human cultured glioblastoma cells

Low-dose cisplatin protects human neuroblastoma SH-SY5Y cells from paclitaxel-induced apoptosis

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