Effect of traditional Chinese medicine (Sairei‐to) on monoclonal antibody‐induced proteinuria in rats

Abstract

The effects of traditional Chinese medicine (Sairei‐to) on monoclonal antibody (mAb) inducing proteinuria were examined. Four hundred, 200 and 100mglkg body weight (BW) of Sairei‐to and phosphate‐buffered saline (PBS) as a control were injected intraperitoneally into four groups of female Wistar rats every day from 5 days before intravenous injection of mAb to the end of the experimental period. The amount of urinary protein excretion was significantly suppressed in roughly a dose‐dependent manner. For example, 116.6 ± 89.7mg/day of proteinuria was observed in control groups compared to 4.2 ± 15.2 mglday in the 400 mglkg BW of Sairei‐to treated group 2 days after mAb injection (P < 0.01). Statistically significant (P <0.01) differences were again observed in a repeat experiment (122.1 ± 53.7 vs 10.2 ± 10.1 mglday on the 2nd day) without affecting the glomerular filtration rate. No significant difference was recognized between the total amount of mAb bound to glomeruli 1 h after mAb injection in Sairei‐to‐treated and non‐treated rats, indicating that Sairei‐to pretreatment has no effects on the number or quality of antigenic molecules. The effect of Sairei‐to on a non‐immunological model of proteinuria was also examined. No significant reduction of proteinuria by similar Sairei‐to treatment was observed in aminonucleoside of puromycin nephropathy. The authors conclude that mAb‐induced proteinuria in rats is significantly suppressed by the traditional Chinese medicine, Sairei‐to.

Regulation of podocalyxin expression in the kidney of streptozotocin-induced diabetic rats with Chinese herbs (Yishen capsule)

“…Other Chinese herbs such as Rheum palmatum (chishao) [33], Salviae miltiorrhizae (danshen) [34], Astragalus membranaceus (huangqi) and Angelica sinensis (dangui) [35] could also have anti-oxidative activity and help to recover kidney function in ischemia/reperfusion injury or nephrotic syndrome. Moreover, a traditional Chinese herbal formula - Sairei-to (chailingtang) has been shown to have protective effects on kidney injury due to its suppression of mesangial cell proliferation [36]. Therefore, not only single herb but also combination of herbs has renal protective property.…”

Therapeutic use of traditional Chinese herbal medications for chronic kidney diseases

“…Saireito also decreased urinary protein excretion in a rat model of subtotal nephrectomy 46,47 . However, Saireito did not provide any additional benefit when added to enalapril for renal protection in rats with subtotal nephrectomy 48 .…”

Effects of Saireito, a Japanese herbal medicine, on edema via antagonistic actions against aldosterone in anti-GBM nephritic rats

“…3,4 WKY rats were randomly assigned to groups such that the mean body weight in all groups was almost the same, and then 0.38 ml/100 g of antirat GBM goat serum was intravenously injected through the tail vein. The following day, 6.5 mg of goat γ-globulin was emulsified with Freund's incomplete adjuvant, and intracutaneously administered to the calces of the bilateral posterior limbs.…”

“…1,2 Previously, we and many other investigators have reported that Saireito was effective in various disease models in basic studies of kidney disease. 3,4 However, few studies have investigated its effects on renal edema. To clarify the mechanism of the effects of Saireito on edema, further basic studies should be conducted.…”