Effect of traditional chinese medicine (TCM) and its fermentation using Lactobacillus plantarum on ceftriaxone sodium-induced dysbacteriotic diarrhea in mice

Guo, Xin; Yan, Zipeng; Wang, Jixiang; Fan, Xiaolan; Kang, Jie; Niu, Ruiyan; Sun, Zilong

doi:10.1186/s13020-022-00575-x

Cited by 10 publications

(9 citation statements)

References 49 publications

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“…Intestinal flora is an interactive complex ecosystem, and its species and types have a great impact on the health of the host ( 28 ). Moreover, it has a variety of basic functions, including carbohydrate metabolism, interaction with the immune system, and prevention of pathogen invasion ( 29 ). However, the abuse of antibiotics will lead to the increase of drug resistance of some bacteria, which in turn will upset the balance of intestinal flora and lead to a series of adverse reactions such as diarrhea ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Bao

et al. 2022

Front. Nutr.

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Diarrhea is one of the common adverse reactions in antibiotic treatment, which is usually caused by the imbalance of intestinal flora, and probiotics play an important role in the structure of intestinal flora. Therefore, this experiment studied the regulatory effect of Lactiplantibacillus plantarum 2-33 on antibiotic-associated diarrhea (AAD) mice. First, the AAD mice model was established by the mixed antibiotic solution of gentamicin sulfate and cefradine. Then, the physiological indexes and diarrhea of mice were observed and recorded by gastric perfusion of low dose (1.0 × 107 CFU/ml), medium dose (1.0 × 108CFU/ml), and high dose (1.0 × 109 CFU/ml) strain 2-33. 16S rRNA gene V3-V4 regions were sequenced in colon contents of mice in control group, model group, self-healing group, and experimental group, respectively, and the diversity of intestinal flora and gene function prediction were analyzed. The results showed that the intestinal flora of AAD mice was not significantly regulated by gastric perfusion of strain 2-33 to 7 days, but the relative abundance and diversity of intestinal flora of AAD mice were significantly improved by gastric perfusion to 14 days (p < 0.05). In addition, at the genus level, the relative abundance of Lactobacillus increased significantly, and the relative abundance of Enterococcus and Bacillus decreased significantly (p < 0.05). In addition, the regulation of strain 2-33 on intestinal flora of AAD mice was time- and dose-dependent, short-term gastric perfusion, and low dose had no significant effect (p > 0.05). Strain 2-33 can significantly increase the levels of anti-inflammatory cytokines IL-4 and IL-10, significantly decrease the levels of proinflammatory cytokines TNF-α and IFN-γ (p < 0.05), and can also adjust carbohydrate metabolism, amino acid metabolism, and energy metabolism to normal levels, thus accelerating the recovery of intestinal flora structure of AAD mice. In summary, strain 2-33 can improve the structure and diversity of intestinal flora of AAD mice, balance the level of substance and energy metabolism, and play a positive role in relieving diarrhea, maintaining and improving the intestinal microecological balance.

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Section: Discussionmentioning

confidence: 99%

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Bao

et al. 2022

Front. Nutr.

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“…With the overuse of antibiotics, a series of side effects are caused, such as antibiotic-associated diarrhea (AAD), which imposes more economic burden on the patient [ 47 ]. To our knowledge, the cause of AAD is commonly identified as intestinal flora disturbance [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…The damage of intestinal mucosal barrier dysfunction can be also an important reason for AAD [ 47 ]. As one of the marker enzymes in many mammalian intestinal mucosal epithelial villus cells, DAO levels can objectively present the degree of intestinal mucosal damage [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…By conducting a systematic review, Zheng et al [ 9 ] found that TCM can make an influence on modulating gut microbiota and improving glucose metabolisms in T2DM patients. Guo et al [ 47 ] have demonstrated that Buzhongyiqi decoction (BD), Sijunzi decoction (SD), and Shenlingbaizhu decoction (SHD) can alleviate diarrhea symptoms induced by ceftriaxone sodium in animal experiments by balancing intestinal flora and improving villi structure. Hui et al [ 48 ] reported that Qiweibaizhusan can restore and adjust the diversity of bacteria in mice intestine with diarrhea syndrome caused by administrating the mixture of gentamycin sulfate and cefradine.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Traditional Chinese Medicine on Treating Antibiotic-Associated Diarrhea in Children: A Systematic Review and Meta-Analysis

Sun

Chen

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Due to the limited treatment options in antibiotic-associated diarrhea (AAD) in children, more effective treatments should be explored. Traditional Chinese medicine (TCM) has a long history in China, which has produced a pretty effect in clinical practice. Many randomized clinical trials (RCTs) have explored the effect of traditional Chinese medicine on treating AAD in children. However, there has been no systematic review or meta-analysis on the impact of TCM on AAD in children. The aim of this study was to systematically review RCTs on the effect of TCM in children with AAD. Methods. RCTs in the past ten years on TCM for AAD in children were included. We searched Electronic databases as much as possible. This paper was registered in PROSPERO (CRD42022301034). Results. 26 studies were included in this systematic review. 25 studies reported the effects of TCM interventions on the total effective rate (RR = 1.20, CI 1.16 to 1.24; p < 0.001 ). 7 studies reported the effects of TCM interventions on the time to change the shape of feces (MD = −1.37, CI −1.67 to −1.07; p < 0.001 ). 17 studies reported the effects of TCM interventions (MD = −1.43, CI −1.71 to −1.15; p < 0.001 ). The pooled results showed that there were no significant differences between the two groups in CD3+, CD4+, CD8+, CD4 : CD8, time for bowel sounds to return to normal, hs-CRP, and IgM. There was a significant difference between the two groups in frequency of diarrhea on the third day after TCM intervention, vomiting improvement time, diamine oxidase, IL-8, TNF, IgA, IgG, and average hospital stay. Conclusions. TCM interventions combined with conventional therapy can improve the therapeutic effect of AAD in children. However, future studies are still needed for the low methodological quality.

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“…Fermentation typically refers to varied benign physiological and organic chemistry changes in plants through microorganism metabolism, it could promote the transformation of active ingredients and increase the metabolism of active substances by microorganisms ( Zhao et al, 2021 ). The microbial fermentation technology is widely used in food, medicine, cosmetics, energy ( Hashemi et al, 2021 ; Guo., 2022 ; Mitri et al, 2022 ; Zigoski et al, 2021 ). Recent analysis on vine tea as a fermentation substrate focuses mostly on health care products and other industries, however less on skin effects ( Shimizu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata)

Hussain

et al. 2022

Front. Bioeng. Biotechnol.

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Vine tea (Ampelopsis grossedentata) is a plant resource with good nutritional and medicinal, and is widely consumed in China. This study aimed to develop a functional vine tea fermentation broth using microbial fermentation and cellulase degradation. First, the most suitable probiotics for vine tea fermentation were screened, and the fermentation conditions were optimized. Then, a new cellulase (Cel 906, MW076177) was added to evaluate the changes in the contents of effective substances and to study its efficacy. The results show that saccharomyces cerevisiae Y-401 was identified as the best strain, the optimal fermentation conditions were a time of 94.60 h, feeding concentration of 115.21 g/L, and temperature of about 34.97 °C. The vine tea fermentation broth has a strong inhibitory ability on 2,2′-azinobis3ethylbenzothiazoline-6-sulfonic acid (ABTS) (99.73%), peroxyl (53.15%), superoxide anion radicals (84.13%), and 1,1-Diphenyl-2trinitrophenylhydrazine (DPPH) (92.48%). It has a decent inhibitory impact on the cell viability, tyrosinase activity (32.25%), and melanin synthesis (63.52%) of B16-F10 melanoma cells induced by α-MSH. Inflammatory cell recruitment was reduced in a zebrafish inflammation model. Therefore, this vine tea fermented broth has strong antioxidant, anti-melanoma, and anti-inflammatory effects, and has healthcare potential as a probiotic tea.

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Effect of traditional chinese medicine (TCM) and its fermentation using Lactobacillus plantarum on ceftriaxone sodium-induced dysbacteriotic diarrhea in mice

Cited by 10 publications

References 49 publications

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Effect of Traditional Chinese Medicine on Treating Antibiotic-Associated Diarrhea in Children: A Systematic Review and Meta-Analysis

Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata)

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