Effect of topological cues on material-driven fibronectin fibrillogenesis and cell differentiation

Ballester-Beltrán, José; Cantini, Marco; Lebourg, Myriam; Rico, Patricia; Moratal, David; Garcı́a, Andrés J.; Salmerón‐Sánchez, Manuel

doi:10.1007/s10856-011-4532-z

Cited by 31 publications

(44 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been previously shown that the degree of fibrillogenesis can be modulated on PEA by adsorbing FN from solutions of different concentrations or by using different adsorption times. 20,22 However, in both cases the total amount of FN on the surfaces changes as well, which does not happen with the copolymers shown in this work (Fig. 4a).…”

Section: Discussioncontrasting

confidence: 57%

Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

Mnatsakanyan

Rico

Grigoriou

et al. 2015

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Fibronectin fibrillogenesis is the physiological process by which cells elaborate a fibrous FN matrix. Poly(ethyl acrylate), PEA, has been described to induce a similar process upon simple adsorption of fibronectin (FN) from a protein solution -in the absence of cells -leading to the so-called material-driven fibronectin fibrillogenesis. Poly(methyl acrylate), PMA, is a polymer with very similar chemistry to PEA, on which FN is adsorbed keeping the globular conformation of the protein in solution. We have used radical polymerisation to synthesise copolymers with controlled EA/MA ratio seeking to modulate the degree of FN fibrillogenesis. The physico-chemical properties of the system were studied using dynamicmechanical analysis, differential scanning calorimetry and water contact angle. Both the degree of FN fibrillogenesis and the availability of the integrin binding region of FN directly depend on the percentage of EA in the copolymer, whereas the same total amount of FN was adsorbed regardless the EA/MA ratio. Cell morphology adhesion and differentiation of murine C2C12 were shown to depend on the degree of FN fibrillogenesis previously attained on the material surface. Myogenic differentiation was enhanced on the copolymers with higher EA content, i.e. more interconnected FN fibrils.

show abstract

Section: Discussioncontrasting

confidence: 57%

Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

Mnatsakanyan

Rico

Grigoriou

et al. 2015

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…[[qv: 14a,c,26]] This work builds on this concept by engineering artificial fibrillar microenvironments that exploit the interaction between one of the main structural ECM components, FN, and a particular surface chemistry, PEA. We have previously shown that this acrylate is able to trigger the cell‐free organization of FN molecules into physiological‐like nanofibrils upon adsorption of the protein onto the material surface, regardless of surface topography;18, 27 the resulting biointerface promotes cell adhesion and differentiation in vitro and supports bone regeneration in vivo 19, 28. We have also observed that other proteins or protein fragments affect this material‐driven FN assembly,20, 28 and we have identified VN as a promising candidate to alter FN organization at the cell–material interface 20.…”

Section: Discussionmentioning

confidence: 99%

Vitronectin as a Micromanager of Cell Response in Material‐Driven Fibronectin Nanonetworks

et al. 2017

Self Cite

View full text Add to dashboard Cite

Surface functionalization strategies of synthetic materials for regenerative medicine applications comprise the development of microenvironments that recapitulate the physical and biochemical cues of physiological extracellular matrices. In this context, material‐driven fibronectin (FN) nanonetworks obtained from the adsorption of the protein on poly(ethyl acrylate) provide a robust system to control cell behavior, particularly to enhance differentiation. This study aims at augmenting the complexity of these fibrillar matrices by introducing vitronectin, a lower‐molecular‐weight multifunctional glycoprotein and main adhesive component of serum. A cooperative effect during co‐adsorption of the proteins is observed, as the addition of vitronectin leads to increased fibronectin adsorption, improved fibril formation, and enhanced vitronectin exposure. The mobility of the protein at the material interface increases, and this, in turn, facilitates the reorganization of the adsorbed FN by cells. Furthermore, the interplay between interface mobility and engagement of vitronectin receptors controls the level of cell fusion and the degree of cell differentiation. Ultimately, this work reveals that substrate‐induced protein interfaces resulting from the cooperative adsorption of fibronectin and vitronectin fine‐tune cell behavior, as vitronectin micromanages the local properties of the microenvironment and consequently short‐term cell response to the protein interface and higher order cellular functions such as differentiation.

show abstract

“…Electrospun fibers of PEA were collected as described elsewhere [26]. Briefly, PEA 1 % Benzoin was dissolved in hexafluoroisopropanol (HFIP, Sigma) at 20 mg/mL.…”

Section: Electrospinningmentioning

confidence: 99%

“…The fibrillar organization of FN upon passive adsorption on PEA was named material-driven fibrillogenesis, since the assembled FN fibrils on PEA share some similarities with cell-assembled FN matrices [23]. In addition, the resulting fibrillar FN structure on PEA recapitulates the native structure of FN matrices and displays enhanced biological activity [23,26]. Figure 1c shows the FN network assembled upon adsorption on PEA observed with AFM in comparison with globular organization of FN on glass.…”

Section: Dorsal and Ventral Stimulationmentioning

confidence: 99%

Dorsal and Ventral Stimuli in Cell–Material Interactions: Effect on Cell Morphology

et al. 2012

Self Cite

View full text Add to dashboard Cite

Cells behave differently between bidimensional (2D) and tridimensional (3D) environments. While most of the in vitro cultures are 2D, most of the in vivo extracellular matrices are 3D, which encourages the development of more relevant culture conditions, seeking to provide more physiological models for biomedicine (e.g., cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. In this study, cells were cultured between two protein coated surfaces (sandwich-like culture). Cells used both dorsal and ventral receptors to adhere and spread, undergoing morphological changes with respect to the 2D control. Combinations of fibronectin and bovine serum albumin on the dorsal and ventral sides led to different cell morphologies, which were quantified from bright field images by calculating the spreading area and circularity. Although the mechanism underlying these differences remains to be clarified, excitation of dorsal receptors by anchorage to extracellular proteins plays a key role on cell behavior. This approach-sandwich-like culture-becomes therefore a versatile method to study cell adhesion in well-defined conditions in a quasi 3D environment.

show abstract

Effect of topological cues on material-driven fibronectin fibrillogenesis and cell differentiation

Cited by 31 publications

References 48 publications

Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

Controlled Assembly of Fibronectin Nanofibrils Triggered by Random Copolymer Chemistry

Vitronectin as a Micromanager of Cell Response in Material‐Driven Fibronectin Nanonetworks

Dorsal and Ventral Stimuli in Cell–Material Interactions: Effect on Cell Morphology

Contact Info

Product

Resources

About