Effect of topical insulin on cutaneous wound healing in rats with or without acute diabetes

Apikoğlu-Rabuş, Şule; İzzettin, Fikret; Turan, Pınar; Ercan, Feríha

doi:10.1111/j.1365-2230.2009.03419.x

Cited by 63 publications

(71 citation statements)

References 18 publications

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“…Organization of collagen fibers was greater in the diabetic group treated with topical insulin (DI group) than in the other groups. Our results are consistent with those of other studies that have shown that topical insulin enhances wound healing by increasing collagen deposition (Apikoglu-Rabus et al, 2010;Madibally et al, 2003).…”

Section: Discussionsupporting

confidence: 95%

Effect of Topical Insulin on Second-Degree Burns in Diabetic Rats

Azevedo

Pessoa

Moreira

et al. 2015

Biological Research For Nursing

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The healing process is complex in diabetic wounds, and the healing mechanism of burn wounds is different from that of incisional or excisional wounds. Data from our previous study indicated that topical insulin cream reduced wound closure time in diabetic rats. Our aim was to investigate the effect of topical insulin cream on wound healing following second-degree burns in control and diabetic rats. Rats were divided into four groups: control (nondiabetic) rats treated with placebo (CP), control (nondiabetic) rats treated with topical insulin cream (CI), diabetic rats treated with placebo (DP), and diabetic rats treated with topical insulin cream (DI). The wounds were assessed at 4 time points (1, 7, 14, and 26 days) post-wounding for morphometric analysis of wound sections stained with hematoxylin/eosin, α-smooth muscle actin, and Picrosirius red to evaluate general aspects of the wound, inflammatory infiltrate, blood vessels, and Types I and III collagen fibers. Histological analysis showed that topical insulin cream increased the inflammatory cell infiltrate in the DI group (at 7 and 14 days postburn, p< .05) and blood vessels (at 14 days postburn, p < .05) to levels similar to those of groups CP and CI. Wounds treated with topical insulin cream (CI and DI groups) showed significantly stronger staining for fibrillar collagen than wounds of the DP group. The use of topical insulin may reduce the duration of the inflammatory phase; improve wound reepithelialization, tissue granulation, and wound contraction; and increase collagen deposition in second-degree burns in healthy and diabetic animals.

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Section: Discussionsupporting

confidence: 95%

Effect of Topical Insulin on Second-Degree Burns in Diabetic Rats

Azevedo

Pessoa

Moreira

et al. 2015

Biological Research For Nursing

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“…Our results suggest a characteristic spatial distribution and evolution of perfusion changes that may be exploited to influence local or topical delivery strategies of therapeutic agents such insulin or silver nanoparticles [45, 46], and help understand the regional biodistribution of systemically administered drugs. In vivo LSCI-based flow and vascularization metrics could also provide a non-invasive indication of the degree of wound healing.…”

Section: Discussionmentioning

confidence: 99%

In vivo laser speckle imaging reveals microvascular remodeling and hemodynamic changes during wound healing angiogenesis

et al. 2011

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Laser speckle contrast imaging (LSCI) is a high-resolution and high contrast optical imaging technique often used to characterize hemodynamic changes in short-term physiological experiments. In this study, we demonstrate the utility of LSCI for characterizing microvascular remodeling and hemodynamic changes during wound healing angiogenesis in vivo. A 2 mm diameter hole was made in the mouse ear and the periphery of the wound imaged in vivo using LSCI over 12 days. We were able to visualize and quantify the vascular and perfusion changes that accompanied wound healing in the microenvironment proximal to the wound, and validated these changes with histology. We found that consistent with the stages of wound healing, microvessel density increased during the initial inflammatory phase (i.e., day 0–3), stayed elevated through the tissue formation phase (i.e., until day 7) and returned to baseline during the tissue remodeling phase (i.e., by day 12). Concomitant ‘‘wide area mapping’’ of blood flow revealed that tissue perfusion in the wound periphery initially decreased, gradually increased from day 3–7, and subsided as healing completed. Interestingly, some regions exhibited a reestablishment of tissue perfusion approximately 6 days earlier than the ∼ 18 days usually reported for the long term remodeling phase. The results from this study demonstrate that LSCI is an ideal platform for elucidating in vivo changes in microvascular hemodynamics and angiogenesis, and has the potential to offer invaluable insights in a range of disease models involving abnormal hemodynamics, such as diabetes and tumors.

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“…After that, the insulin diffuses through the interior of the fibers to its surface and across the boundary layer, reaching the external medium. Regularity in insulin release in vitro is promising for obtaining an artificial insulin depot with controllable and continued activity that may be used topically for better healing of diabetic' wounds [34] or for transdermal and subcutaneous insulin delivery [35].…”

Section: Kinetics Of Insulin Release From Dac-insulin Complexmentioning

confidence: 99%

Oxidized cotton as a substrate for the preparation of hormone-active fibers-characterization, efficiency and biocompatibility

et al. 2015

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Oxidized cellulose has a long history of safe and effective use in medical applications. In this paper, research has been directed towards obtaining hormone-active cellulose fibers in the form of an artificial insulin depot, and examination of its biocompatibility regarding cytotoxicity, sensitization, and irritation. The procedure of obtaining the fibrous insulin depot is based on the modification of cotton fibers with sodium periodate, followed by chemisorption of insulin from insulin aqueous solutions. In order to optimize the insulin chemisorption process, the influence of the fiber structure parameters, i.e. the aldehyde group content and iodine sorption value (ISV) on the chemisorption capacity was examined. The obtained artificial depot, containing ≈55 mg insulin/g of fibers, has been characterized in vitro by investigation of the desorption kinetics of the insulin from the fibrous depot. It has been shown that insulin is controllably released in quantities of 1.3-1.6 mg of insulin during 24 hours, in the course of 20 days. The results of biocompatibility tests have shown that the examined artificial depot neither shows irritating effects nor provokes sensitizing or cytotoxic effects. Therefore, these materials are acceptable for use in a direct contact with tissue of a living organism.

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Effect of topical insulin on cutaneous wound healing in rats with or without acute diabetes

Abstract: This study revealed that topical insulin application to cutaneous wounds accelerates wound healing in rats with or without acute diabetes.

Cited by 63 publications

References 18 publications

Effect of Topical Insulin on Second-Degree Burns in Diabetic Rats

Effect of Topical Insulin on Second-Degree Burns in Diabetic Rats

In vivo laser speckle imaging reveals microvascular remodeling and hemodynamic changes during wound healing angiogenesis

Oxidized cotton as a substrate for the preparation of hormone-active fibers-characterization, efficiency and biocompatibility

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