Effect of TNF‑α on the proliferation and osteogenesis of human periodontal mesenchymal stem cells

Abstract

The aim of the present study was to investigate the effect of tumor necrosis factor-α (TNF-α) on the proliferation and osteogenesis of human periodontal mesenchymal stem cells (hPDLSCs). Antigen expression in hPDLSCs was detected by flow cytometry. hPDLSCs were divided into four groups: A control group with no TNF-α treatment, and three experimental groups treated with 0.1, 1 and 10 ng/ml TNF-α, respectively. The effect of TNF-α on proliferation of hPDLSCs in vitro was detected using a Cell Counting Kit-8 assay. Differentiation into an osteogenic lineage was detected by alkaline phosphatase sand alizarin red staining, and the mRNA and protein expression levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and type I collagen (Col-I) were detected using reverse transcription-quantitative PCR and western blot respectively. Following treatment with 10 ng/ml TNF-α, proliferation was significantly increased compared with an untreated control group (P<0.01). Additionally, there was a significant inhibition of alkaline phosphatase enzyme activity, alizarin red mineralization node size, and in the gene and protein expression levels of osteogenic differentiation markers, including Runx2, OCN and COL-I (all, P<0.05). Taken together, the results indicated that treatment with 10 ng/ml TNF-α promoted the proliferation of hPDLSCs in vitro and inhibited osteogenic differentiation of hPDLSCs, providing an experimental basis for regulation of hPDLSC-mediated periodontal tissue regeneration.

Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway

“…PDLSCs are regarded as the main functional cells for periodontal tissue repair and regeneration, which are influenced by macrophage polarization. However, in periodontitis, the osteogenic capacity of PDLSCs was severely impaired due to inflammatory cytokines [ 44 ]. Prior studies showed that the CM of M2 macrophages promoted PDLSC osteogenic and cementoblastic differentiation through Akt and JNK signaling [ 45 ], while the CM of M1 macrophages impaired osteogenesis [ 46 ].…”

Vitamin D Enhanced the Osteogenic Differentiation of Cell Spheroids Composed of Bone Marrow Stem Cells

“…This research analyzed the effects of vitamin D on the osteogenic differentiation and mineralization of human mesenchymal stem cells. Differentiation into an osteogenic lineage was detected by alkaline phosphatase activity, and the mRNA levels of RUNX2, BSP, OCN, and COL1A1 were detected using real-time quantitative polymerase chain reaction [26].…”

Reactive morphology of dividing microglia following kainic acid administration

“…This secondary damage can include a prolonged inflammatory response, increased permeability of the blood-brain barrier, cytotoxicity, apoptosis, excitotoxity, and demyelination ( Kuno et al, 2005 ), all of which prolong microglial reactivity ( Smith et al, 2012 ). TNF-α is also involved with signaling pathways that control cell division in a variety of cell types ( Cao et al, 2021 ; Niu et al, 2021 ; Skartsis et al, 2021 ). We hypothesized that eliminating TNF-α from microglia by genetic knockout (KO) would reduce microglial cell division and activation following an inflammatory trigger.…”