Effect of TNF-α concentration on selected clinical parameters of swine after burns

Abstract

IntroductionThe study aimed to observe TNF-α serum concentration as well as changes in respiration rate, body temperature, and pulse rate in burn victims during 84 h post burn.Material and MethodsA total of 30 healthy pigs were divided into two groups: A, the test group and N, the control group. The experimental group suffered burns to 30% of the body surface, and after infliction of the burns both groups were closely monitored.ResultsThe biggest increase in TNF-α serum concentration in the test subjects occurred around the 6th h of the study, and the second biggest increase took place between 12th and 36th h. In the 36th h, TNF-α was 2.5 times more concentrated in serum in the test group than in the control group. In the test group, the biggest increase in respiration rate occurred up to the 6th h post burn, on average up to 29/min. In the 12th h post burn, the mean pulse rate in the test group was 133/min and dropped to the lowest value in the 72nd h of the experiment. A gradual increase in body temperature up to 41.72°C was observed up to the 30th h post burn and decreased to a significant value of 40.74°C by the 84th h of the study.ConclusionIn a period of a pronounced rise in TNF-α serum concentration, this parameter, pulse rate, and respiration rate are highly correlated and are also influenced by multiple inflammation forming factors.

Current understanding of thermo(dys)regulation in severe burn injury and the pathophysiological influence of hypermetabolism, adrenergic stress and hypothalamic regulation—a systematic review

“…Pigs are mostly used to study aspects such as wound healing [ 44 , 45 ] and body temperature is only occasionally recorded; however, it has been shown to increase from baseline after burn injury induction [ 46 ]. Also, increased body temperature in swine as an indicator for systemic inflammatory response syndrome was described in a study researching the burn’s effects on the whole organism [ 47 ] as well as during the inflammatory processes after burn [ 48 ]. Sheep are very commonly used to study inhalation injuries after burn trauma [ 49–51 ] but not frequently in researching thermoregulation.…”

Effect of early excision of a burn wound on the activity of selected enzymes in pig serum

“…The anesthetic protocol followed procedures described in previous reports (1). Burns were performed with a softwarecontrolled hotplate in a precisely defined contact area on a flank of the body equal to 1% of the body surface area in a 50 kg pig (2). The hotplate weighing 2.5 kg rested on the animal's body for 10 seconds without any additional pressure being applied (Polish patent no.…”