Effect of titanium carbide coating on the osseointegration response in vitro and in vivo

Brama, Marina; Rhodes, Nicholas P.; Hunt, John A.; Ricci, A.; Teghil, R.; Migliaccio, Silvia; Rocca, Carlo Della; Leccisotti, S; Lioi, Attilio; Scandurra, Marta; Maria, G. De; Ferro, D.; Pu, Fanrong; Panzini, Gianluca; Politi, Laura; Scandurra, Roberto

doi:10.1016/j.biomaterials.2006.08.018

Cited by 125 publications

(69 citation statements)

References 26 publications

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“…HOS cells were cultured in Dulbecco's modified Eagle medium (DMEM) at 37 °C, while hFOB1.19 cells were grown in DMEM/ F12 in the presence of 0.3 mg/ml G418 at 33.5 °C (Brama et al, 2007). All cell cultures were supplemented with 10% (v/v) fetal bovine serum (GIBCO, Carlsbad, CA, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin, and maintained in a humidified atmosphere of 5% (v/v) CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Peng

et al. 2011

J. Zhejiang Univ. Sci. B

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This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo. MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose-and time-dependent manner. Based on the findings of light and transmission electron microscopy, Hoechst 33258 staining, and fluorescein isothiocyanate (FITC)-annexin V staining, the cytotoxicity of ART in HOS cells occurs through apoptosis. With ART treatment, cytosolic cytochrome c was increased, Bax expression was gradually upregulated, Bcl-2 expression was downregulated, and caspase-9 and caspase-3 were activated. Thus, the intrinsic apoptotic pathway may be involved in ART-induced apoptosis. Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G 2 /M phase. In nude mice bearing HOS xenograft tumours, ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin, in agreement with in vitro observations. ART has a selective antitumour activity against human osteosarcoma HOS cells, which may be related to its effects on induction of apoptosis via the intrinsic pathway. The results suggest that ART is a promising candidate for the treatment of osteosarcoma.

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Section: Cell Culturementioning

confidence: 99%

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Peng

et al. 2011

J. Zhejiang Univ. Sci. B

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“…[4][5][6][7] Among these methods, chemical treatment is a relatively simple and effective technique and has been widely investigated. [8][9][10][11] In previous studies, a type of nanorod structure with a phase composition of anatase and rutile has been fabricated on a titanium surface using different chemical processes.…”

Section: Introductionmentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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Background and methods:Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin β1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results:The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion: This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo.

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“…Because of this reason, several studies appear aiming to find a biocompatible material without producing an adverse local or systemic biological response [1]. Among several materials, titanium is the most used in dental and orthopedic implants because of its higher properties such as biocompatibility, modulus of elasticity higher than bone, and high resistance to corrosion when compared to other metals and metallic alloys [2,3].…”

Section: Introductionmentioning

confidence: 99%

Stem cells behaviour on titanium surface modificated by plasma

Sá¹,

Silva²,

Rocha³

et al. 2012

BDS

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Over the last decade, a considerable development of new technologies to the modification of dental implants has been observed contributing to reduce the healing process and their use in areas with low bone density. Among the new techniques, plasma nitriding has showed excellent results. In this study, a superficial modification of commercial pure titanium (Degree II), by using two different plasma treatments (planar and hollow cathode nitriding) was accomplished aiming at an optimization of the surface for biomedical applications. An evaluation of the chemical composition in all samples was carried out, in addition to a study of their roughness and texture. Then, stem cells were deposited onto these surfaces and a comparison among their properties and their biological behavior was accomplished. The results showed that the nitriding techniques produced significant changes in the superficial texture of the Ti samples. The roughness test presented better results in samples nitrided by hollow cathode technique. Statistically significant differences were found in the cell proliferation of samples nitrided by hollow cathode nitriding when compared to samples without treatment. The used techniques were, therefore, effective and directly influenced the characteristics of the titanium surface and consequently, the stem cell behavior.

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Effect of titanium carbide coating on the osseointegration response in vitro and in vivo

Cited by 125 publications

References 26 publications

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Stem cells behaviour on titanium surface modificated by plasma

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