Effect of Tissue Factor on Invasion Inhibition and Apoptosis Inducing Effect of Oxaliplatin in Human Gastric Cancer Cell

Abstract

Objective: Tissue factor (TF) is expressed abnormally in certain types of tumor cells, closely related to invasion and metastasis. The aim of this study was to construct a human gastric cancer cell line SGC7901 stably-transfected with human TF, and observe effects on oxaliplatin-dependent inhibition of invasion and the apoptosis induction. Methods: The target gene TF was obtained from human placenta by nested PCR and introduced into the human gastric cell line SGC7901 through transfection mediated by lipofectamine. Stably-transfected cells were screened using G418. Examples successfully transfected with TF-pcDNA3 recombinant (experimental group), and empty vector pcDNA3 (control group) were incubated with oxaliplatin. Transwell chambers were used to show change in invasive ability. Caspase-3 activity was detected using a colorimetric method and annexin-V/PI doublestaining was applied to detect apoptosis. Results: We generated the human gastric cancer cell line SGC7901/TF successfully, expressing TF stably and efficiently. Compared with the control group, invasion increased, whereas caspase-3 activity and apoptosis rate were decreased in the experimental group. Conclusion: TF can enhance the invasive capacity of gastric cancer cells in vitro. Its increased expression may reduce invasion inhibition and apoptosis-inducing effects of oxaliplatin and therefore may warrant targeting for improved chemotherapy.

The XPD Lys751Gln Polymorphism has Predictive Value in Colorectal Cancer Patients Receiving Oxaliplatin-Based Chemotherapy: a Systemic Review and Meta-analysis

“…Oxaliplatin is a cytotoxic platinum compound, and the mechanism of action of oxaliplatin is known to induce intra-stand crosslinking in DNA, leading to inhibition of DNA synthesis and apoptosis of cancer cells (Faivre et al, 2003;Yu et al, 2012). Thus, DNA repair capability is a key factor that modulates sensitivity to oxaliplatin.…”

Anticancer effect of cucurbitacin B on MKN-45 cells via inhibition of the JAK2/STAT3 signaling pathway

“…Tumor cells possess unlimited replication potential; and the occurrence of tumors is associated with the abnormal proliferation and differentiation of cells in addition to abnormal apoptosis (8). Loss of tumor cells via apoptosis is considered to be a contributing factor in tumor therapy and cancer molecular biology (8). There are two major apoptotic pathways, initiated by caspase-9 and caspase-8 respectively, which can activate caspase cascades (29).…”

“…Cell apoptosis is programmed cell death mediated by various signal transduction pathways under certain physiological or pathological conditions, in which multiple genes are involved. As a major pathway to clear abnormally proliferating cells, cell apoptosis may be intentionally induced to treat cancers (7,8). Chemotherapy medications combined with traditional Chinese medicine preparations may greatly enhance the therapeutic effect and prevent postoperative relapse and metastasis (9,10).…”

Effect of tissue factor knockdown on the growth, invasion, chemoresistance and apoptosis of human gastric cancer cells

“…Previous studies have found that TF shows abnormal expression levels in various types of tumor cell, including gastric, colon and pancreatic cancer cells (9,10), and that its expression levels are closely associated with biological behaviors of tumor cells, including growth, invasion and metastasis, which were confirmed by the present study. In vitro and in vivo functional studies (11–13) have shown that TF significantly promotes tumor angiogenesis and enhances the invasion and metastasis abilities of tumor cells. In a previous study, a recombinant vector with the pcDNA3.1/TFcDNA plasmid was transfected into SGC7901 human gastric cancer cells, and the endogenous increased expression levels of TF significantly increased the levels of invasion and metastasis of the cells, and thus confirmed that TF was closely associated with the processes of invasion and metastasis of cancer (14).…”

“…In tumor-associated vascular diseases, the expression levels of TF in the circulation increase. For example, in human colon cancer, the TF in the circulation and on the membrane surface is associated with the occurrence of tumor cells and blood vessels (13). Tesselaar et al (19) also demonstrated that MP-associated TF was involved in the development of tumor-associated thrombosis in various types of adenocarcinoma.…”