Effect of Tinospora Cordifolia on the Antitumor Activity of Tumor-Associated Macrophages-Derived Dendritic Cells

Abstract

We and others previously have reported that extract prepared from medicinal plant Tinospora cordifolia shows a wide spectrum of immunoaugmentary effects. Tinospora cordifolia was shown to upregulate antitumor activity of tumor-associated macrophages (TAM). In this article we present evidence to show that an alcoholic extract of Tinospora cordifolia (ALTC) enhances the differentiation of TAM to dendritic cells (DC) in response to granulocyte/macrophage-colony-stimulating factor, interleukin-4, and tumor necrosis factor. DC differentiated in vitro from TAM that were harvested from tumor-bearing mice after i.p. administration of ALTC (200 mg/kg body weight) 2 days posttumor transplantation shows an enhanced tumor cytotoxicity and production of tumoricidal soluble molecules like TNF, IL-1, and NO. Adoptive transfer of these TAM-derived DC to Dalton's lymphoma-bearing mice resulted in prolongation of survival of tumor-bearing mice. This is the first report regarding the differentiation and antitumor functions of TAM-derived DC obtained from tumor-bearing host administered with ALTC. The possible mechanisms involved also are discussed.

Modulation of Natural Killer Cell Activity, Antibody-Dependent Cellular Cytotoxicity, and Antibody-Dependent Complement-Mediated Cytotoxicity by Andrographolide in Normal and Ehrlich Ascites Carcinoma-Bearing Mice

“…1 Tumor growth itself is associated with the onset of immunosuppression and concomitant inhibition of antitumor function of the host immune system. 2 Therefore, use of agents that modify the host immune system by inhibiting this immunosuppressive factors and reverse tumor-induced immunosuppression along with the conventional therapy may provide beneficial therapeutic effects.…”

Effects of Tinospora cordifolia supplementation on semen quality and hormonal profile in rams

“…cordifolia is basically an immuno-modulator (Nair et al, 2004) and also has anti-cancer (Singh et al, 2005), nerve cell protecting (Rawal et al, 2004), anti-diabetic, blood cholesterol-lowering (Stanley et al, 2003) and liverprotective (Bishayi et al, 2002) actions. T. cordifolia has a wide variety of antioxidant properties and reduces reactive oxygen and nitrogen species (ROS/RNS; Veena et al, 2002).…”

Comparative assessment of in vitro antidiabetic activity of Tinospora cordifolia stem, leaves and its callus extracts

“…It has a long history of use in Ayurvedic medicine (the traditional medicine of India). Evidence hints that Tinospora may have anti-cancer (Singh et al, 2005;Singh et al, 2004), immune stimulating (Rawa let al, 2004), anti-diabetic (Stanely et al, 2003;Rathi et al, 2002), cholesterol-lowering (Stanely et al, 2003 and liver-protective (Bishayi et al, 2002) actions. T. cordifolia has also shown some promising speed in healing the diabetic foot ulcers (Purandare et al, 2007).…”