EFFECT OF THYROXINE UPON SUCCINIC OXIDASE SYSTEM OF HEART MUSCLE AND ADRENAL CORTEX OF DOG &lt;I&gt;IN VITRO&lt;/I&gt;

Katsura, Hirosumi

doi:10.1507/endocrj1954.1.109

Cited by 4 publications

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“…As has been pointed out in the preceding paper by Katsura(1954), the effect of thyroxine upon the system of respiratory enzymes has been variously reported by different workers, no consistent information being offered with this respect. Assuming that such a circumstance might be due to difference in experimental conditions, the author cited made observations on the effect of the hormone upon succinic oxidase system as a whole in homogenates of several organs of the dog such as heart muscle, adrenal cortex and thyroid with special reference to the effect of hormone concentration in a wide range.…”

mentioning

confidence: 86%

Effect of Thyroxine Upon Succinic Dehydrogenase Activity as Observed by Means of an Improved Thunberg Technique

Sugisawa¹

1955

Endocrinol Japon

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Effect of Thyroxine Upon Succinic Dehydrogenase Activity as Observed by Means of an Improved Thunberg Technique

Sugisawa¹

1955

Endocrinol Japon

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“…The composition of the reaction mixture was as follows:In the main tube:1) 0.1M K2HPO4-KH2PO4 buffer (PH 7.4) 1.3 ml., 2) 0.2% homogenate 0.6ml., 3) fig. 1, B).…”

Section: Methodsmentioning

confidence: 99%

Effect of Thyroid Hormones and Their Metabolic Products Upon Succinic Dehydrogenase Activity of Mouse Heart Muscle in Vitro

Yamamoto

Ishikawa

1959

Jpn.J.Physiol

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As to the effect of thyroxine upon succinic dehydrogenase activity, some observations have been made in this laboratory employing an improved Thunberg technique. Thyroxine was learned to be effective in a diphasic way in the sense that the hormone in its low concentration range activated succinic dehydrogenase, while inhibitory effect resulted at its relatively high concentrations, when homogenates of mouse and dog tissues were used as the enzyme sources (6, 7). Further detailed work was done on the mode of action of thyroxine upon the same enzyme of rat liver and heart muscle under various experimental conditions, in which concentrations of homogenate and substrate, succinate, were changed (11). Following up these observations, experiments were extended in order to test whether triiodothyronine, and the acetic and propionic acid analogues of thyroxine and triiodothyronine show a similar effect upon succinic dehydrogenase of heart muscle homogenate of mouse . MATERIALS AND METHODSAdult male mice of ddN strain, weighing approximately 20g. each, were employed.Animals were sacrificed by decapitation and heart muscle was excised, weighed on a torsion balance, homogenized with 500 volumes of distilled water using the whole-glass homogenizer, and the homogenate obtained was used for enzyme source.All these procedures were carried out at room temperature.The activity of succinic dehydrogenase was measured employing Thunberg tubes after Yamamoto and Sugisawa (11). The composition of the reaction mixture was as follows:In the main tube:1) 0.1M K2HPO4-KH2PO4 buffer (PH 7.4) 1.3 ml., 2) 0.2% homogenate 0.6ml., 3) hormone solutions of various Received for publication October 24, 1958.

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“…In his recent paper, Katsura(1954) Mouse Whole Lever Homogenate As the crude enzyme source of succinic oxidase system, whole liver homogenate was used for the present purpose of study. Liver was taken out from decapitated mouse, and homogenized after the method of Potter and Elvehjein(1946) directly after excision.…”

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Effect of Thyroxine Upon Significant Components of Succinic Oxidase System in Mouse Liver Homogenate

Suzuki

1954

Endocrinol Japon

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EFFECT OF THYROXINE UPON SUCCINIC OXIDASE SYSTEM OF HEART MUSCLE AND ADRENAL CORTEX OF DOG <I>IN VITRO</I>

Abstract: It is generally accepted that the respiratory enzyme systems which involve the succinic oxidase system are activated by administration of thyroxine in vivo.

Cited by 4 publications

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Effect of Thyroxine Upon Succinic Dehydrogenase Activity as Observed by Means of an Improved Thunberg Technique

Effect of Thyroxine Upon Succinic Dehydrogenase Activity as Observed by Means of an Improved Thunberg Technique

Effect of Thyroid Hormones and Their Metabolic Products Upon Succinic Dehydrogenase Activity of Mouse Heart Muscle in Vitro

Effect of Thyroxine Upon Significant Components of Succinic Oxidase System in Mouse Liver Homogenate

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