Effect of Thyroxine and Propylthiouracil in Ehrlich Ascitic Tumor Cells

Abstract

The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effect.

Anti-proliferative and apoptotic efficacy of diallyl disulfide on Ehrlich ascites carcinoma

“…The current in vivo study revealed that DADS has a potential antiproliferative and cytotoxicity effect against EAC (a transplantable neoplasia from a malignant epithelium that corresponds to mammary adenocarcinoma) in EAC-bearing female mice. [14,21] This activity was confirmed by a significant decrease in EAC-aliquot volume and total and alive EAC-cell number, and a tremendous increase in dead EAC-cell count and percent as a result of treatment with DADS in comparison to a vehicle. The anti-tumor cytotoxicity of DADS was associated with an improved survival and increased the life span of EAC-bearing mice.…”

“…[38][39][40] Thus, it should not be excluded that DADS may have anti-angiogenic effects which in turn may have a crucial role in anti-proliferative effects. [15] As suggested by Freitas et al [14] and Senger et al, [41] the production of ascitic extracellular fluid in Ehrlich carcinoma is said to occur due to increased capillary permeability present in the peritoneal cavity. This vascular change occurs due to increased receptor expression for autocrine motility factor (AMF).…”

“…This vascular change occurs due to increased receptor expression for autocrine motility factor (AMF). [14] AMF link to its receptor induces angiogenesis and changes in endothelial cell morphology causing a subsequent increase in vascular permeability with increased amount of ascitic fluid [42] in addition to the increasing number of proliferated cells. In the present study, the volume of EAC-aliquot relative to total cell count was increased as a result of DADS treatment.…”

“…Total number of EAC-cells and percent of dead EAC-cells were calculated for each EAC-bearing mouse. This procedure was adopted from the methods of Freitas et al [14] and Chandru et al [15] Blood samples were obtained from carotid artery of each mouse into ethylenediaminetetraacetic acid (15%)-containing tubes under mild diethyl ether anesthesia and were centrifuged at 3,000 g for 15 min. The plasma was aspirated into Eppendorf tubes and kept in deep freezer at -30 °C until used for plasma sialic acid determination.…”

Evaluation of Antitumor Activity of Cuscuta Reflexa Roxb (Cuscutaceae) Against Ehrlich Ascites Carcinoma in Swiss Albino Mice

“…Susceptibility of erythrocytes to peroxides was confirmed by the elevated WBC count, reduced hemoglobin and RBC count, as observed in the control group. The constituents of the extracts probably facilitated improvement in hematological parameters by scavenging reactive oxygen species and improving the antioxidant defense system, thus indicating that the extracts exerted haematopoietic protective activity as well as antioxidant property [24,25].…”