Effect of thyroid status on lipid composition and peroxidation in the mouse liver

Guerrero, Adán; Pamplona, Reinald; Portero‐Otín, Manuel; Barja, Gustavo; López-Torres, Mónica

doi:10.1016/s0891-5849(98)00173-7

Cited by 102 publications

(76 citation statements)

References 38 publications

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“…PTU inhibits functions of the thyroid hormones that are among the most important factors involved in the basal metabolic rate of almost both organs including liver and heart (Guerrero et al, 1999;Ganong, 2005). Thyroid abnormalities are also considered as physiological modulators of in vivo cellular oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…Group I animals receiving simple drinking water served as control, whereas those of group II, III, IV and V received only PTU (0.05% in drinking water for 5 weeks) ( Guerrero et al, 1999;Messarah et al, 2010;Jena et al, 2012). On 30 th day, animals of group III, IV and V received different doses of PQQ (1, 5 and 10 mg/ kg / day for six days, respectively) along with PTU as administered to group II animals.…”

Section: Experimental Designmentioning

confidence: 99%

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Regulation of oxidative stress and lipid peroxidation in PTU induced mice, treated with Pyrroloquinoline quinone (PQQ)

Kumar¹

2013

IOSR-JPBS

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Regulation of oxidative stress and lipid peroxidation in PTU induced mice, treated with Pyrroloquinoline quinone (PQQ)

Kumar¹

2013

IOSR-JPBS

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“…Different results could be attributed to assay type and sensitivity and the fact that lipid peroxidation is more transient in nature when compared to other forms of oxidative damage. There are reports showing that lipid peroxidation is not altered in hypothyroid mice (64) or rats (65) compared to euthyroid animals.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial oxidant generation and oxidative damage in Ames dwarf and GH transgenic mice

Brown‐Borg

Johnson

Rakoczy

et al. 2001

AGE

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Aging is associated with an accumulation of oxidative damage to proteins, lipids and DNA. Cellular mechanisms designed to prevent oxidative damage decline with aging and in diseases associated with aging. A long-lived mouse, the Ames dwarf, exhibits growth hormone deficiency and heightened antioxidative defenses. In contrast, animals that over express GH have suppressed antioxidative capacity and live half as long as wild type mice. In this study, we examined the generation of H202 from liver mitochondria of Ames dwarf and wild type mice and determined the level of oxidative damage to proteins, lipids and DNA in various tissues of these animals. Dwarf liver mitochondria (24 months) produced less H202 than normal liver in the presence of succinate (p<0.03) andADP (p<0.003). Levels of oxidative DNA damage (8OHdG) were variable and dependent on tissue and age in dwarf and normal mice. Forty-seven percent fewer protein carbonyls were detected in 24-month old dwarf liver tissue compared to controls (p<0.04). Forty percent more (p<0.04) protein carbonyls were detected in liver tissue (3-month old) of GH transgenic mice compared to wild types while 12 month old brain tissue had 53% more protein carbonyls compared to controls (p<0.005). Levels of liver malonaldehyde (lipid peroxidation) were not different at 3 and 12 months of age but were greater in Ames dwarf mice at 24 months compared to normal mice. Previous studies indicate a strong negative correla-

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“…Both clinical [2,3] and experimental [1,4,5] studies have demonstrated that hyperthyroidism can cause an elevation in oxidative stress, most likely because of increased mitochondrial oxygen consumption. Mitochondria are the main production site of free oxygen radicals in healthy tissues because the leak of these radicals from the mitochondrial respiratory chain and the free oxygen radical production rate can be directly related to the rate of mitochondrial oxygen consumption [6]. The superoxide (O 2 .-), hydroxyl (HO), perhydroxyl radicals (HO 2 -) and nitric oxide (NO) are the most important free radicals that are derived from oxygen [7].…”

Section: Introductionmentioning

confidence: 99%

Dose-Dependent Protective Effect of L-Carnitine on Oxidative Stress in the Livers of Hyperthyroid Rats

Yıldırım

Dane

et al. 2013

EAJM

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Objective: The present study was designed to investigate the dosedependent protective effect of L-carnitine (LC) on thyroid hormoneinduced oxidative stress in rat liver tissue. Materials and Methods:Twenty-one male Sprague Dawley rats were divided into four groups: control, hyperthyroidism, hyperthyroidism plus L-carnitine 100, and hyperthyroidism plus L-carnitine 500. Hyperthyroidism was induced in rats by injecting 250 μg of L-thyroxine/ kg body weight/day for twenty consecutive days. The activities of catalase (CAT), glutathione peroxidase (GPX) and myeloperoxidase (MPO) and the level of malondialdehyde (MDA) were measured in liver homogenates. Results:The liver CAT, GPX and MPO activities were significantly lower in the hyperthyroid rats than in the control group. Treating hyperthyroid rats with both low-dose (100 mg/kg) and high-dose (500 mg/kg) L-carnitine for 10 days resulted in a marked increase in the activities of the antioxidant enzymes in the liver tissue. Conclusion:The present study indicates that the low-dose L-carnitine application was sufficient to prevent L-thyroxine-induced oxidative stress in rat livers.Key Words: Catalase, glutathione peroxidase, L-carnitine, Liver, Malondialdehyde, Myeloperoxidase Özet Amaç: Bu çalışma rat karaciğer dokusunda tiroid hormonu ile uyarı-lan oksidatif stres üzerine L-karnitinin doza bağlı koruyucu etkilerini araştırmaktı. Gereç ve Yöntem:Yirmi bir erkek Sprague Dawley rat 4 gruba bö-lündü: Kontrol, hipertroidi, hipertiroidi + L-karnitine 100 ve hipertiroidi + L-karnitine 500. Hipertiroidi, yirmi gün boyunca ratlara 250 μg / kg dozunda L-Thyroxine enjeksiyonu uygulanarak oluşturuldu. Karaciğer homojenatlarında katalaz (CAT), glutatyon peroksidaz (GPX), myeloperoksidaz (MPO) aktiviteleri ve malondialdehit (MDA) düzeyleri ölçüldü. Bulgular:Kontrollerle karşılaştırıldığında hipertiroidili ratlarda karaciğer CAT, GPX ve MPO aktiviteleri belirgin olarak azaldı. 10 gün boyunca hem düşük doz (100 mg/kg) hem de yüksek doz (500 mg/ kg) L-karnitin uygulaması hipertiroidili ratlarda karaciğer antioksidan enzim aktivitelerinde belirgin bir artışa yol açtı.Sonuç: Bu çalışmanın sonuçları L-Tiroksin ile uyarılan rat karaciğerin-deki oksidatif stresi önlemek için düşük doz L-karnitin uygulamasının yeterli olduğunu göstermektedir.

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Effect of thyroid status on lipid composition and peroxidation in the mouse liver

Cited by 102 publications

References 38 publications

Regulation of oxidative stress and lipid peroxidation in PTU induced mice, treated with Pyrroloquinoline quinone (PQQ)

Regulation of oxidative stress and lipid peroxidation in PTU induced mice, treated with Pyrroloquinoline quinone (PQQ)

Mitochondrial oxidant generation and oxidative damage in Ames dwarf and GH transgenic mice

Dose-Dependent Protective Effect of L-Carnitine on Oxidative Stress in the Livers of Hyperthyroid Rats

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