Effect of thymoquinone on P53 gene expression and consequence apoptosis in breast cancer cell line

Dastjerdi, Mehdi Nikbakht; Mehdiabady, Ebrahim Momeni; Iranpour, Farhad Golshan; Bahramian, Hamid

doi:10.4103/2008-7802.180412

Cited by 63 publications

(34 citation statements)

References 41 publications

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“…In the last two decades, many naturally occurring substances have been reported to prevent the occurrence of carcinogenesis at various stages (Pitchaiah et al, 2017;Lefranc et al, 2017). In this regard, TQ has shown its huge potential to protect against experimental carcinogenesis and molecular mechanism in various cancer cell lines and different model animals of cancer (Rajput et al, 2013;Salim et al, 2013;Dastjerdi et al, 2016;Khan et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone Augments Cyclophosphamide-Mediated Inhibition of Cell Proliferation in Breast Cancer Cells

Khan

Aldebasy

Alsuhaibani

et al. 2019

Asian Pac J Cancer Prev

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Section: Discussionmentioning

confidence: 99%

Thymoquinone Augments Cyclophosphamide-Mediated Inhibition of Cell Proliferation in Breast Cancer Cells

Khan

Aldebasy

Alsuhaibani

et al. 2019

Asian Pac J Cancer Prev

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“…Recent reports suggest Tq to be effective in adjuvant cancer therapies (Mostofa, Hossain, Basak, & Sayeed, 2017). The effect of Tq has been proven against a variety of cancer types which include the cancer of bladder (Iskender, Izgi, & Canatan, 2016), breast (Dastjerdi, Mehdiabady, Iranpour, & Bahramian, 2016), colon (Chen et al, 2015) lung (Yang, Kuang, Lv, & Yan, 2015), and prostate (Richards, Jones, Hughes, Benghuzzi, & Tucci, 2006). However, the effect of this phytochemical on renal carcinoma cells and its mechanistic investigations need further research.…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone attenuates phosphorylation of AKT to inhibit kidney cancer cell proliferation

Dera

Rajagopalan

2019

J Food Biochem

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Thymoquinone (Tq) is an active compound from Nigella sativa which is used in traditional medicine. The effect of Tq on kidney cancer and the pathway of action remain unproven. Herein, we report the anticancer properties of Tq on kidney cancer cells. Tq demonstrated anti‐proliferative effects in A498 cells with a GI50 value of 40.07 µM and Caki‐1 cells with a GI50 of 51.04 µM by the MTT assay. Tq exhibited nuclear fragmentation and inhibited trans‐endothelial migration of A498 and Caki‐1 cells in a dose‐responsive manner. Time‐dependent increase in Annexin V‐positive cells and sub‐G0/G1 cell population was observed post‐Tq treatment. The compound increased Bax protein levels and reduced Bcl‐2 protein levels dose dependently in cells, thereby favoring apoptosis. Tq decreased the phosphorylation of Akt in both kidney cell types. The results suggest effective anticancer activity of Tq on kidney cancer cells which may be mediated by the Akt pathway. Practical applications Results from the current investigation will through more light on the mechanistic pathway of Tq activity on the inhibition of kidney cancer cell proliferation. The output of this preclinical in vitro study may be translated into better chemotherapeutics of Tq and its analogs to treat kidney cancer. However, a detailed investigation on in vivo models is recommended.

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“…9,11-17 However, research done on breast cancer cells was limited in the usefulness of its results by either use of excessively high concentrations of TQ on only a single form of breast cancer cells, as seen in one study, or the use of a lipid carrier molecule alongside TQ in another study, which may have affected the results. 14,15 In order to find a minimal effective dosage of TQ in preventing the growth of aggressive breast cancer cells, as done with blackseed oil as seen above, without the interference of a lipid carrier molecule, this study utilized lower concentrations of TQ on both cell types. Figure 1C displays the growth of the MCF-7 cell lines treated with 1.0 μM and 5.0 μM thymoquinone over the span of a week.…”

Section: Discussionmentioning

confidence: 99%

“…5 Intriguingly, TQ alone has even been seen to display cancer-fighting ability against cancers of the larynx, colorectal area, brain, breast, oral lining, blood, and a number of other cancers. 9,[11][12][13][14][15][16][17] In the study testing the effects of TQ on the non-aggressive MCF-7 breast cancer line, however, it was noted that very high concentrations of TQ were used as treatments (ranging from 0 to 80 μM, at 10 μM increments). 14 This leads to a lack of knowledge regarding minimal effective dosage of the chemical.…”

Section: Introductionmentioning

confidence: 99%

Blackseed (Nigella sativa) Oil and its Active Ingredient, Thymoquinone, Suppress the Aggressive Phenotype of Breast Cancer Cells

Chaudhry¹,

Siddiqui²,

Steffen³

et al. 2017

AJUR

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Breast cancer is a leading cause of cancer deaths in women within the United States. However, current treatment methods for the disease present deleterious side effects themselves. Therefore, there is a move towards finding natural cures in order to mitigate negative side effects while still providing effective treatment for the cancer. Blackseed (Nigella sativa) oil is one particular natural remedy, alongside its active ingredient thymoquinone (TQ), which has been successfully tested for suppressing certain types of breast cancer cell proliferation. TQ itself has been seen to be capable of preventing proliferation of both non-aggressive MCF-7 and highly aggressive MDA-MB-231 cancer cells. However, studies which looked at the effects of TQ on MCF-7 cells alone were limiting in their use of high concentrations of the chemical without emphasis on finding a minimum effective dosage. Additionally, a second study which tested the effects of TQ on both MCF-7 and MDA-MB-231 cell lines conducted the experiments in the presence of a lipid-carrier molecule. This, in turn, may have served as a confounding variable in the results. Therefore, it was hypothesized that a minimal effective dosage for both blackseed oil and TQ could be determined, where a significantly greater suppression of MDA-MB-231, in comparison to MCF-7, cell proliferation would be observed. Cell proliferation, cell adhesion, and soft agar assays were used to test the hypothesis of this study. The minimum effective dosage for each substance, characterized by proliferation of the non-aggressive MCF-7 cells to some extent and suppression of the aggressive MDA-MB-231 cells, were determined to be 0.5 µL for blackseed oil and 1.0 µM for TQ. Additionally, TQ’s effectiveness was noted to be more time-dependent than blackseed oil. This study supports the use of minimal effective doses for blackseed oil or TQ to naturally treat breast cancer while preventing damage to non-aggressive cells. KEYWORDS: Breast cancer; Blackseed oil; Nigella sativa; Thymoquinone; Effective dose; Natural remedies

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Effect of thymoquinone on P53 gene expression and consequence apoptosis in breast cancer cell line

Cited by 63 publications

References 41 publications

Thymoquinone Augments Cyclophosphamide-Mediated Inhibition of Cell Proliferation in Breast Cancer Cells

Thymoquinone Augments Cyclophosphamide-Mediated Inhibition of Cell Proliferation in Breast Cancer Cells

Thymoquinone attenuates phosphorylation of AKT to inhibit kidney cancer cell proliferation

Blackseed (Nigella sativa) Oil and its Active Ingredient, Thymoquinone, Suppress the Aggressive Phenotype of Breast Cancer Cells

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