Effect of Three Days Starvation on the Phagocytic Activity of Rat Peritoneal Macrophages

Salman, Hertzel; Bergman, Michael; Bessler, Hanna; Alexandrova, S.; Straussberg, Rachel; Zahavi, Izhar; Djaldetti, M

doi:10.1159/000040856

Cited by 13 publications

(7 citation statements)

References 10 publications

(9 reference statements)

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“…De la Fuente et al (1992) observed impaired peritoneal macrophage function in mice fed a low-protein diet for 15 days. We have shown that the phagocytic activity of peritoneal macrophages from rats kept on water only for 3 days was decreased, whereas their ability to generate superoxide anion was markedly increased (Salman et al, 1998b). Since the serum corticosterone level of the fasting animals was found to be increased, it was suggested that the impaired macrophage function is hormonally mediated.…”

Section: Certain Conditions Affectingmentioning

confidence: 95%

Phagocytosis—The mighty weapon of the silent warriors

Djaldetti

Salman

Bergman

et al. 2002

Microscopy Res & Technique

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Professional phagocytes, comprising polymorphonuclear neutrophils and monocyte/macrophage cells, play an important role in the host defense. Any defect in their function exposes the organism to microbial intruders terminating in fatal diseases. The functional responses of the phagocytes to bacterial and fungal infections include chemotaxis, actin assembly, migration, adhesion, aggregation, phagocytosis, degranulation, and reactive oxygen species production. Superoxide generation by phagocytic NADPH oxidase is an imperative step toward bacterial killing. Phagocytes participate in inflammatory reactions and exert tumoricidal activity. They are supported by serum factors such as immunoglobulins, cytokines, complement, the acute phase reactant C-reactive protein, production of antibacterial proteins, and others. In addition to their principal task to eliminate bacteria, they are engaged in removing damaged, senescent, and apoptotic cells. Engulfed cell debris, large particles such as latex beads, fat, and oil droplets, are examples of phagocytic activity illustrated in the present review with transmission and scanning electron microscope micrographs. Numerous factors, such as diseases and stressful conditions, affect the engulfing activity of the professional phagocytes. Our experience regarding the impaired phagocytic capacity of cells in patients with diabetes and chronic renal failure is discussed. The results obtained in our laboratory from experiments detecting the effect of strenuous physical exercise, hypothermia, fasting, and abdominal photon irradiation on the phagocytic capacity of human polymorphonuclear neutrophils and rat peritoneal macrophages are hereby summarized and the reports on those subjects in the recent literature are reviewed. A variety of assays are applied for quantifying phagocytosis. Flow cytometry based on incubation of phagocytic cells with fluorescent conjugated particles and measuring the amount of fluorescence as an indicator of the engulfing capacity of the cells is a useful method. A direct visualization of the ingested particles using light or electron microscopy is a valuable tool for estimation of phagocytic function. In our hands, the use of semithin sections of embedded phagocytes following their incubation with latex particles provided satisfactory results for measuring the total number of phagocytic cells, as well as the internalizing capacity of each individual cell. Microbiological assays, the nitroblue tetrazolium test, quantitation of antibody- and antigen-mediated phagocytosis, as well as methods reviewed in detail in other reports are additional applications for determination of this intricate process.

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Section: Certain Conditions Affectingmentioning

confidence: 95%

Phagocytosis—The mighty weapon of the silent warriors

Djaldetti

Salman

Bergman

et al. 2002

Microscopy Res & Technique

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“…There is an extensive list of diseases that will result in decreased intake or increased energy requirements including but not limited to dental and oral cavity diseases, gastric ulcers, enteritis, colitis, peritonitis, liver disease, pneumonia, pleuritis, dysphagia, endocrinopathies and sepsis . Regardless of the inciting cause, if long‐lasting, negative energy and nitrogen balance will lead to weight loss, muscle loss, lipid mobilisation, a decrease in immune system competence and prolongation of recovery from illnesses due to decrease in efficacy of therapeutic interventions; ultimately resulting in an increased cost or even poorer prognosis . During feed deprivation, the metabolic rate, thyroxin synthesis and glycogen stores decrease .…”

Section: Introductionmentioning

confidence: 99%

Effect of feed deprivation on selected parameters of lipid mobilisation and hepatic function in healthy Akhal Teke horses

Tóth¹,

Auth²,

Rompos³

et al. 2017

Equine Veterinary Journal

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“…We chose noise as a model of stress because the effect of noise on the immune responses have never been studied in humans, whatever the age, and the first results obtained in young mice (8)(9)(10) suggested that this model could be particularly relevant to the study of the influence of stress on macrophage functions.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies that have examined the relationship between stress and immunity have dealt with the effects of stress on lymphocytes or NK cells of adult humans or experimental animals (1)(2)(3)(4)(5). Only a few, however, have explored the association between stress and macrophage function (6,7), and still fewer, the effects of stress on the immune response of old animals or humans (8)(9)(10). Moreover, most experimental studies have used acute or chronic stresses that are not encountered by humans in daily life, such as electric shock, cold water immersion or water deprivation.…”

Section: Introductionmentioning

confidence: 99%

Effect of an auditory stress on peritoneal and alveolar cells in C57 BL/6J mice of advanced age

Wazières

Harraga²,

Spehner³

et al. 2000

Luminescence

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The aim of this study was to analyse the effects of auditory stress on peritoneal and alveolar macrophages in C57 BL/6J mice of advanced age, and to compare the results to those obtained in old mice submitted to a sham stress, and to those observed in young mice submitted to the same auditory stress. We used a chemiluminescence assay to measure the production of free oxygen radicals (FOR) by macrophages. Eight 22 month-old mice were exposed to a sound stress of 110 dB for three consecutive nights; nine were submitted to a sham stress. The results were compared to those obtained in young (8 week-old) mice, 21 submitted to noise stress, and 17 controls. The corticosterone level was not increased after stress in any group. FOR production in old mice was significantly higher than that in young mice. Stress did not induce significant changes in FOR production by alveolar cells in young mice; however, the FOR production by alveolar cells was significantly higher in the stressed group than in the control group of old mice. These results show that noise stress is associated with modifications of macrophage functions that are influenced by cell localization, the behaviour of alveolar and peritoneal macrophages of old mice being clearly different in our experimental model.

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Effect of Three Days Starvation on the Phagocytic Activity of Rat Peritoneal Macrophages

Cited by 13 publications

References 10 publications

Phagocytosis—The mighty weapon of the silent warriors

Phagocytosis—The mighty weapon of the silent warriors

Effect of feed deprivation on selected parameters of lipid mobilisation and hepatic function in healthy Akhal Teke horses

Effect of an auditory stress on peritoneal and alveolar cells in C57 BL/6J mice of advanced age

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