Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats

Abstract

Background and Objective: Exposure of the brain to Ischemia-Reperfusion (I/R) may cause tissue damage through oxidative stress.Thiamine pyrophosphate (TPP), which has a protective effect against oxidative stress, is the active metabolite of vitamin B 1 . In this study, the protective effect of TPP against possible I/R damage of brain tissue was investigated. Materials and Methods: Thirty rats were randomly divided into BIR, TIR and HG groups consisting of ten rats. In the TIR group, 20 mg kgG 1 TPP was injected intraperitoneally (ip). After 1 hr, clips were placed in the common carotid arteries of the BIR and TIR groups under anesthesia. Brain tissue was subjected to ischemia for 10 min. Afterward, the clips were opened and 3 hrs of reperfusion was achieved. In the HG group, only subcutaneous incisions were made and closed. Then, the brain tissues removed by euthanasia were biochemically analyzed. Results: While, I/R increased malondialdehyde (MDA), myeloperoxidase (MPO), Tumor Necrosis Factor-" (TNF-"), Interleukin-1$ (IL-1$) and 8-Hydroxyguanine (8-OHGua) levels in brain tissues, total caused a decrease in glutathione (tGSH), glutathione peroxidase (GPO) and glutathione reductase (GSHRd) levels (p<0.001). The TPP applied before I/R significantly prevented these changes (p<0.05). Conclusion: The results of biochemical tests suggested that TPP may be beneficial in preventing possible brain damage due to I/R.

Lacidipine, thiamine pyrophosphate and their combination on the ocular ischemic syndrome induced by bilateral common carotid artery ligation

“…TPP (20 mg/kg) was applied intraperitoneally (ip) in the TCCU and LTCCU. TPP at these doses has been previously used in a brain I/R model in rats [13] . In addition, it has been demonstrated that TPP administered 1h before the cerebral hypoxia model procedure in rats crosses the blood brain barrier and reaches the brain and has a healing effect [14] .…”