Effect of Thermal Manipulation During Incubation and Spraying Japanese Quail Eggs With Ascorbic Acid on Embryogenesis and Phsiological Responses of Hatch Chicks

Abstract

The objective of this study was to investigate the impact of spraying ascorbic acid on Japanese quail eggs submitted to high temperature during4 th to 14 th days of incubation period on embryogenesis, hatchability, embryonic mortality rate and chick quality.Six hundred Japanese quaileggs were divided into two equal groups of 300 eggs each according to incubation temperature. In the first group: eggs were incubated at 37.5°C (thermoneutral incubation temperature NIT 37.5°C)and 65% relative humidity (RH)from 1 st to 14 th days of incubation. The second group: (high incubation temperatureHIT 41°C): Eggs were incubated at 37.5°C during the first 3 days of incubation and from day 4 th to 14 th incubation temperature (IT) was raised to 41°C and RH65% for three hours per day. The eggs were evenly divided into three ascorbic acid (AA) treatments. The first one was sprayed with distilled water (control AA0), while, the second and third groups were sprayed with solutions containing 15 and 30gAA/L, respectively.The results concluded that HIT group had the highest embryo weight, embryonic mortalities and lower hatchability% compared to NIT group. The chicks weight (g) and triiodothyronine (T3) concentration were significantly lower (P≤0.05) in HIT group compared toNIT group. Glucose level and heterophil: lymphocyte (H: L) ratio wereincreased significantly (P≤0.01) as affected by thermal manipulations.The embryonic development, embryonic viability as well as hatchability improved by spraying ascorbic acid as compared to the untreated group.Values of glucose, T3, RBCs, PCV% and HGB were significantly increased in the blood of hatching chicks by spraying AA solutionas compared to control while, residual yolk and H: L ratio was significantly(P≤0.05) decreased by spraying AA solution . The embryonic development, internal organs%, PCV% and HGB has been affected by interaction between incubation temperature and ascorbic acid. It could be concluded that, spraying eggs of Japanese quail exposed to heat stress with AA solutions (15 or 30 g/L) daily during4 th to 14 th days of incubation period, may be an alternative method to minimize embryos heat stress and maximize the embryonic viability as well as hatchability, immunity of hatched chicks and chick quality.

Embryonic thermal manipulation of poultry birds: Lucrative and deleterious effects

“…Another recent study on quail found that intermittent TM (+1.7°C, 12 h/d) during ED0‐13 improved thermotolerance during heat challenge (36°C for 7 h) at 35 days of age (Vitorino Carvalho et al, 2020). Similar to chicken but not identical, quails physiologically cope with the heat stress, with a modification of several metabolic and haematologic parameters, including an elevation in plasma corticosterone concentration and a reduction in plasma thyroid hormone levels (Abdel‐Fattah, 2019; El‐Daly et al, 2013; Porto & Fontenele‐Neto, 2020; Vitorino Carvalho et al, 2020). Correspondingly, the surface temperature of the shank was decreased, suggesting a modulation of the blood flow to sustain the interior temperature.…”