Effect of Thermal Injury and Immunosuppression on the Dissemination of Candida albicans from the Mouse Gastrointestinal Tract

Ekenna, Okechukwu; Fader, Robert

doi:10.1097/00004630-198903000-00007

Cited by 7 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is possible that the solid nature of the chow, which partially protects the fungus from gastric acidity, could have accounted for our ability to achieve this long-term C. albicans colonization. Most previously reported animal experiments used a suspension rather than a solid form of food containing C albicans to feed the animals (7,8,14,28). The role of gastric acidity in the prevention of Candida colonization and infection has been recognized previously.…”

Section: Cuxmentioning

confidence: 99%

“…Disseminated candidiasis is an increasing problem in immunocompromised patients (3,19,25). The source of dissemination in most of these patients is either an indwelling intravenous catheter or the gastrointestinal tract (3,5,7,8,15,19). Experimental and clinical data suggest that Candida organisms can pass into the bloodstream by persorption through the intact gastrointestinal mucosa and spread to visceral organs, leading to systemic candidiasis (5,13,14,16,24,25).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A model of sustained gastrointestinal colonization by Candida albicans in healthy adult mice

et al. 1990

View full text Add to dashboard Cite

Three-month-old male Crl:CD1(ICR)BR and C3H/HeJ mice were fed chow containing Candida albicans for 14 days, while similar control mice were fed regular food. Stool cultures were done for all mice before and after administration of the special diet. Stool cultures were repeated 48 h, 1 week, and 1 and 3 months after stopping the diet for Crl:CD1(ICR)BR mice and again 5 months afterward for C3H/HeJ mice. Some animals were sacrificed at the end of the special diet, and cultures and histopathologic examination of various organs were performed. Colonization with C. albicans occurred in the Candida-fed mice, and the fungus was maintained in the gastrointestinal tract at a concentration of 103 to 104 CFU/g of stool for up to 5 months. There was no histologic evidence of organ infection with Candida spp. The fungus was not found in stool cultures or organs of mice in the control group. The results suggest that persistent gastrointestinal colonization of adult mice by C. albicans can be achieved without immunosuppression. Thus, with additional manipulations, this model could be useful for studying the role of gastrointestinal colonization by C. albicans in the development of systemic infection.

show abstract

Section: Cuxmentioning

confidence: 99%

mentioning

confidence: 99%

A model of sustained gastrointestinal colonization by Candida albicans in healthy adult mice

et al. 1990

View full text Add to dashboard Cite

show abstract

“…This gastrointestinal dissemination corresponded to the day of severest neutropenia. 9 The legal medicine autopsy suggests an esophagealgastric source of infection. A possible source may have been external contamination of the nasogastric tube or enteral nutrition.…”

Section: Discussionmentioning

confidence: 99%

Fulminant, Undetected Candida Sepsis After an Apparently Survivable Burn Injury

Struck¹,

Stiller²,

Corterier³

et al. 2009

Journal of Burn Care & Research

View full text Add to dashboard Cite

We report a 20-year-old male who suffered smoke inhalation injury and burns covering 26% of his TBSA, including his face, dorsal chest, and both the arms. The Abbreviated Burn Severity Index was 5 (likelihood of survival 95%). He underwent burn surgery, requiring massive transfusion. Postoperatively, he appeared increasingly hyperthermic, showed respiratory exhaustion, and was neutropenic (lowest white blood cell count was 0.8 Gpt with a normal granulocyte count). He developed acute respiratory distress syndrome, renal failure, and severe inflammatory response syndrome. Aggressive ventilation patterns, intermittent prone positioning, and high-dose catecholamine therapy were performed. Hydrocortisone therapy and antibiotic prophylaxis did not improve his clinical status. He died after 12 days of septic multiple organ failure. Legal medicine autopsy identified aggressive Candida famata mycosis. The organism mainly affected the alimentary canal, and there were multiple pyemic abscesses in tissues of the heart, liver, spleen, kidneys, lungs, and meninges. Histology confirmed gastric ulcers as the source of the Candida infection. Despite the autopsy findings, all intravital specimens collected (blood, urine, and tracheal mucus) and all clinical Candida antigen tests were unsuspicious. Postoperative neutropenia may be a warning sign of severe infection even in survivable burns. Suppression of immune response and possible previous gastric Candida colonization may contribute to hazardous outcomes. However, delayed and unreliable methods to detect fungal infections remain a major problem in burn care. Occult aggressive fungal sepsis resulting in early multiple organ failure should be kept in mind.

show abstract