Effect of Theobroma cacao L. on the Efficacy and Toxicity of Doxorubicin in Mice Bearing Ehrlich Ascites Carcinoma

Patil, Priyanka; Khanal, Pukar; Patil, Vishal S.; Charla, Rajitha; Harish, Darasaguppe R.; Patil, B. M.; Roy, Subarna

doi:10.3390/antiox11061094

Cited by 12 publications

(4 citation statements)

References 65 publications

(65 reference statements)

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“…In the present study, we observed the amelioration of various oxidative stress biomarkers in multiple organs after COE co-treatment with doxorubicin. Moreover, our previous studies also support the efficacy of COE in ameliorating oxidative stress and cancer progression ( Patil et al, 2022b ; 2022a ). Emmanuel et al reported that cocoa leaf polyphenolic-rich extract inhibited xanthine oxidase due to the flavonoids and phenolic acids present in the extract ( Irondi et al, 2017 ).…”

Section: Discussionsupporting

confidence: 66%

Computational and experimental pharmacology to decode the efficacy of Theobroma cacao L. against doxorubicin-induced organ toxicity in EAC-mediated solid tumor-induced mice

et al. 2023

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Background and objective: Doxorubicin is extensively utilized chemotherapeutic drug, and it causes damage to the heart, liver, and kidneys through oxidative stress. Theobroma cacao L (cocoa) is reported to possess protective effects against several chemical-induced organ damages and also acts as an anticancer agent. The study aimed to determine whether the administration of cocoa bean extract reduces doxorubicin-induced organ damage in mice with Ehrlich ascites carcinoma (EAC) without compromising doxorubicin efficacy.Methodology: Multiple in vitro methods such as cell proliferation, colony formation, chemo-sensitivity, and scratch assay were carried out on cancer as well as normal cell lines to document the effect of cocoa extract (COE) on cellular physiology, followed by in vivo mouse survival analysis, and the organ-protective effect of COE on DOX-treated animals with EAC-induced solid tumors was then investigated. In silico studies were conducted on cocoa compounds with lipoxygenase and xanthine oxidase to provide possible molecular explanations for the experimental observations.Results:In vitro studies revealed potent selective cytotoxicity of COE on cancer cells compared to normal. Interestingly, COE enhanced DOX potency when used in combination. The in vivo results revealed reduction in EAC and DOX-induced toxicities in mice treated with COE, which also improved the mouse survival time; percentage of lifespan; antioxidant defense system; renal, hepatic, and cardiac function biomarkers; and also oxidative stress markers. COE reduced DOX-induced histopathological alterations. Through molecular docking and MD simulations, we observed chlorogenic acid and 8′8 methylenebiscatechin, present in cocoa, to have the highest binding affinity with lipoxygenase and xanthine oxidase, which lends support to their potential in ameliorating oxidative stress.Conclusion: The COE reduced DOX-induced organ damage in the EAC-induced tumor model and exhibited powerful anticancer and antioxidant effects. Therefore, COE might be useful as an adjuvant nutritional supplement in cancer therapy.

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Section: Discussionsupporting

confidence: 66%

Computational and experimental pharmacology to decode the efficacy of Theobroma cacao L. against doxorubicin-induced organ toxicity in EAC-mediated solid tumor-induced mice

et al. 2023

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“…Mez (= Mespilodaphne sassafras Meisn.) canela-sassafrás, sassafrás essence 105, 108 Root Native c Aromatic root used in therapy (undefined) and essences Antifungal [ 90 ]; anticoagulant [ 91 ] Lecythidaceae Lecythis ollaria L. sapucaia, camari-macaco 85,103 Almonds Exotic Food and medicinal (undefined) Wound healing [ 92 ]; Selenium poisoning (e.g., alopecia) [ 93 , 94 ] Loganiaceae Spigelia anthelmia L. not mentioned 104 Not mentioned Native Poisonous when fresh and vermifuge when dry Anthelmintic † [ 95 , 96 ]; cardiac contraction [ 97 , 98 ]; neuromuscular blockade [ 99 ] Malvaceae Theobroma cacao L. cacaueiro 103 Cacao butter Native Medicinal (undefined) Antioxidant [ 100 , 101 ]; anti-inflammatory [ 102 ]; neuroprotective [ 103 ]; cardioprotective, hepatoprotective and nephroprotective combined with doxorubicin [ 104 ]; antialopecia [ 105 ] Meliaceae Carapa procera DC. (= Carapa guyanensis Oliv.)…”

Section: Resultsmentioning

confidence: 99%

Amazonian useful plants described in the book “Le Pays des Amazones” (1885) of the Brazilian propagandist Baron de Santa-Anna Nery: a historical and ethnobotanical perspective

Silva,

Oliveira,

Baratto

2024

J Ethnobiology Ethnomedicine

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Background Frederico José de Santa-Anna Nery (1848–1901) was a Brazilian Baron who referred to himself as a "volunteer propagandist" for Brazil in Europe, serving as an immigration agent to publicize the living conditions in the Amazon region, advocating for its development and modernization at the end of the nineteenth century. Santa-Anna Nery’s most famous book is "Le Pays des Amazones" (The Lands of the Amazons), first published in 1885, which the author dedicated a chapter to introduce and report on the Amazonian useful plant species and its relationship with humans. The aim of this work is to understand the historical context and ethnobotanical value of the plant species in the Brazilian Amazon at the end of the nineteenth century through an analysis of the book “Le Pays des Amazones” (1885) by Baron de Santa-Anna Nery, as well as to bring to light the historical importance of this very influential propagandist, who has been forgotten nowadays. Methods The original book “Le Pays des Amazones” (1885), as well as the original 3rd edition and its translated version into Portuguese, was carefully analyzed and all information about plants was systematized, with botanical names being updated. Finally, using the scientific name of medicinal plants alone or in combination with their traditional use, a search was carried out in databases in order to indicate current pharmacological studies that provide evidence about the described traditional uses. Results A total of 156 plant species were identified in the book, although 132 species had their scientific names updated. These species belong to 45 different families, with Fabaceae and Arecaceae the most represented, and 109 plants are Brazilian native. Considering only the 36 medicinal plants, the main medicinal indications reported were astringent, purgative/laxative, stimulant and tonic, vermifuge, febrifuge, sudorific, emetic, diuretic and antidysenteric. Regarding other useful plants (non-medicinal), 97 species were cited for food, constructions and buildings, spices and condiments, ornaments and objects, carpentry, textile fibers, gums, oils, balms and essences, pigments and tanning, hunting and fishing. Conclusions When the book “Le Pays des Amazones” is analyzed from a timeless perspective, with a particular focus on historical ethnobotany, it is possible to observe the economic, social, and political importance of many useful plants for the Amazon at the end of the nineteenth century and how the relationship between local people, indigenous communities, and immigrants was established with plant biodiversity.

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“…However, the groups pretreated with extracts and standards counteracted these anomalies, conceivably a result of the decrease in the inflammatory markers suggestive of reduction in the cardiomyocyte injury in the pretreated groups that was consistent with the outcomes described by other researchers. 4 , 36 The ECG parameters are shown in Figure 5 b–f. In RR, PR, QRS, QT, and QTC interval, a significant decrease ( p < 0.01 (low dose extract group) and p < 0.001 (high dose extract group and standard simvastatin and vit E group)) was seen in contrast to the control DOX group.…”

Section: Resultsmentioning

confidence: 99%

Utilization of Quince (Cydonia oblonga) Peel and Exploration of Its Metabolite Profiling and Cardioprotective Potential Against Doxorubicin-Induced Cardiotoxicity in Wistar Rats

Hanan,

Hasan,

Zahiruddin

et al. 2023

ACS Omega

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Quince (Cydonia oblonga Mill.) is a pomaceous fruit that is typically processed into jams, jellies, and marmalade. The byproduct, i.e., the quince peel emanated from the processing industry, can be upcycled, ensuring zero waste policy and resulting in a sustainable food system. In our study, the quince peel was explored for in vitro phytochemical analysis and in vivo cardioprotective potential. Two diverse extractions (ultrasonication and reflux) and four different solvents (aqueous, ethanolic, hydroethanolic, and methanolic) were used for the extraction of quince peel and assessed for the phytochemical and antioxidant study. Among all the evaluated extracts, hydroethanolic quince extract extracted through the reflux extraction method showed the maximum phenolic (27.23 ± 0.85 mg GAE/g DW) and flavonoid (16.5 ± 1.02 mg RE/g DW) content. The maximum antioxidant potential (DPPH) with an IC50 value of 204.8 ± 2.24 μg/mL was noted for the hydroethanolic extract. This best active extract was then subjected to HPTLC, UPLC–MS, mineral, and FTIR analysis to study the metabolic profiling and inorganic composition and to confirm the presence of bioactives. Additionally, the in vivo study was done in rats using doxorubicin (DOX)-induced cardiotoxicity. The rats were given extracts orally at 160 and 320 mg/kg bw for 30 days. ECG analysis was done at the termination of the experiment. Besides this, the lipid profile, blood serum parameters (CK-MB, LDH, AST), and tissue parameters (MDA, SOD, GSH, CAT) were analyzed. The DOX-treated group unveiled a substantial variance (p < 0.001) in all the parameters in contrast to the normal control group and extract control groups. However, the pretreated groups substantially alleviated the DOX-induced changes in all the parameters. Additionally, recuperation in histopathological alterations of the cardiac tissue in contrast to the DOX-induced toxicity was also seen in the pretreated groups. Thus, it could be said that the cardioprotective activity of the quince peel extract attributed to the presence of phytoconstituents counteracted the DOX-induced cardiotoxicity and assisted in the restoration of the cardiac injury in rats.

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Effect of Theobroma cacao L. on the Efficacy and Toxicity of Doxorubicin in Mice Bearing Ehrlich Ascites Carcinoma

Cited by 12 publications

References 65 publications

Computational and experimental pharmacology to decode the efficacy of Theobroma cacao L. against doxorubicin-induced organ toxicity in EAC-mediated solid tumor-induced mice

Computational and experimental pharmacology to decode the efficacy of Theobroma cacao L. against doxorubicin-induced organ toxicity in EAC-mediated solid tumor-induced mice

Amazonian useful plants described in the book “Le Pays des Amazones” (1885) of the Brazilian propagandist Baron de Santa-Anna Nery: a historical and ethnobotanical perspective

Utilization of Quince (Cydonia oblonga) Peel and Exploration of Its Metabolite Profiling and Cardioprotective Potential Against Doxorubicin-Induced Cardiotoxicity in Wistar Rats

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