Effect of the zinc transporter ZupT on the virulence mechanisms of mesophilic <i>Aeromonas salmonicida</i> SRW‐OG1

Wang, Jiajia; Li, Qiu; Huang, Lixing

doi:10.1002/aro2.17

Cited by 2 publications

(2 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Swimming motility of V. splendidus AJ01 was performed according to the previous method (Wang et al 2023 ). V. splendidus AJ01 was grown in medium supplemented with 2.5 μg/mL tryptanthrin for 24 h; then, 10-μL bacterial culture was plugged into the swimming agar, i.e., 2216E medium containing 2.5 μg/mL of tryptanthrin and 0.3% agar.…”

Section: Methodsmentioning

confidence: 99%

Characterization of tryptanthrin as an antibacterial reagent inhibiting Vibrio splendidus

Yang,

Li,

Shi

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Isolates of Vibrio splendidus are ubiquitously presented in various marine environments, and they can infect diverse marine culture animals, leading to high mortality and economic loss. Therefore, a control strategy of the infection caused by V. splendidus is urgently recommended. Tryptanthrin is a naturally extracted bioactive chemical with antimicrobial activity to other bacteria. In this study, the effects of tryptanthrin on the bacterial growth and virulence-related factors of one pathogenic strain V. splendidus AJ01 were determined. Tryptanthrin (10 μg/mL) could completely inhibit the growth of V. splendidus AJ01. The virulence-related factors of V. splendidus AJ01 were affected in the presence of tryptanthrin. Tryptanthrin resulted an increase in biofilm formation, but lead to reduction in the motility and hemolytic activity of V. splendidus cells. In the cells treated with tryptanthrin, two distinctly differentially expressed extracellular proteins, proteases and flagellum, were identified using SDS–PAGE combined with LC–MS. Real-time reverse transcriptase PCR confirmed that the genes involved in the flagellar formation and hemolysin decreased, whereas specific extracellular proteases and the genes involved in the biofilm formation were upregulated. Two previously annotated luxOVs genes were cloned, and their expression levels were analyzed at different cell densities. Molecular docking was performed to predict the interaction between LuxOVs and ATP/tryptanthrin. The two sigma-54-dependent transcriptional regulators showed similar ATP or tryptanthrin binding capacity but with different sites, and the direct competitive binding between ATP and tryptanthrin was present only in their binding to LuxO1. These results indicated that tryptanthrin can be used as a bactericide of V. splendidus by inhibiting the growth, bacterial flagella, and extracellular proteases, but increasing the biofilm. Sigma-54-dependent transcriptional regulator, especially the quorum sensing regulatory protein LuxO1, was determined to be the potential target of tryptanthrin. Key points • Tryptanthrin inhibited the growth of V. splendidus in a dose-dependent manner. • The effect of tryptanthrin on the virulence factors of V. splendidus was characterized. • LuxO was the potential target for tryptanthrin based on molecular docking.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of tryptanthrin as an antibacterial reagent inhibiting Vibrio splendidus

Yang,

Li,

Shi

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The bacterial adhesion assay was performed as the method described by Wang et al (2023). In brief, 50 μL fish mucus was evenly spread over the microscope slides and the slides were let to dry at room temperature overnight.…”

Section: In Vitro Adhesion Assaymentioning

confidence: 99%

Comparative transcriptome analysis explored the molecular mechanisms of a luxR‐type regulator regulating intracellular survival of Aeromonas hydrophila

Chen,

Mao,

Yan

et al. 2024

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

Aeromonas hydrophila is not a traditional intracellular bacterium. However, previous studies revealed that pathogenic A. hydrophila B11 could temporarily survive for at least 24 h in fish phagocytes, and the regulation of intracellular survival in bacteria was associated with regulators of the LuxR‐type. The mechanisms of luxR08110 on the A. hydrophila's survival in macrophages were investigated using comprehensive transcriptome analysis and biological phenotype analysis in this study. The results showed that after luxR08110 was silenced, the intracellular survival ability of bacteria was significantly diminished. Comparative transcriptome analysis revealed that luxR08110 was a critical regulator of A. hydrophila, which regulated the expression of over 1200 genes, involving in bacterial flagellar assembly and chemotaxis, ribosome, sulphur metabolism, glycerolipid metabolism, and other mechanisms. Further studies confirmed that after the inhibition of expression of luxR08110, the motility, chemotaxis and adhesion of A. hydrophila significantly decreased. Moreover, compared with the wild‐type strain, the survival rates of silencing strain were all considerably reduced under both H2O2 and low pH stress conditions. According to both transcriptome analysis and phenotypic tests, the luxR08110 of A. hydrophila could act as global regulator in bacteria intracellular survival. This regulator regulated intracellular survival of A. hydrophila mainly through two ways. One way is to regulate bacterial flagellar synthesis and further affects the motility, chemotaxis and adhesion of bacteria. The other way is to regulate sulphur and glycerolipid metabolisms, thus affecting bacterial energy production and the ability to resist environmental stress.

show abstract

Effect of the zinc transporter ZupT on the virulence mechanisms of mesophilic Aeromonas salmonicida SRW‐OG1

Cited by 2 publications

References 85 publications

Characterization of tryptanthrin as an antibacterial reagent inhibiting Vibrio splendidus

Characterization of tryptanthrin as an antibacterial reagent inhibiting Vibrio splendidus

Comparative transcriptome analysis explored the molecular mechanisms of a luxR‐type regulator regulating intracellular survival of Aeromonas hydrophila

Contact Info

Product

Resources

About