Effect of the urease-derived peptide Jaburetox on the central nervous system of Triatoma infestans (Insecta: Heteroptera)

Galvani, Gerónimo Luis; Fruttero, Leonardo L.; Coronel, Marı́a Florencia; Nowicki, Susana; Demartini, Diogo Ribeiro; Defferrari, Marina S.; Postal, Melissa; Canavoso, Lilián Etelvina; Carlini, Célia R.; Settembrini, Beatriz P.

doi:10.1016/j.bbagen.2014.11.008

Cited by 23 publications

(35 citation statements)

References 43 publications

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“…Despite extensive insecticide application, the disease is still endemic in South America [21]. Using T. infestans as a model, Galvani et al [22] demonstrated that injection of 0.1 μg of JBTX/mg of body weight into adults caused death of all insects in less than 24 h. After 3 h of injection, insects show neurotoxic symptoms such as abnormal behavior of antennae and uncoordinated leg movements, which precede death. JBTX was found to bind to neuronal cells and to interfere with at least two enzymes of the insect brain:

JBTX strongly inhibited the activity of nitric oxide synthase, thereby reducing the levels of the nitric oxide neurotransmitter;

JBTX was found to physically interact with and to increase the activity of UDP-N-acetylglucosamine pyrophosphorylase (UDP-GlcNAcP), an enzyme involved in glycosylation pathways and in the synthesis of chitin [22].

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Section: What’s New?mentioning

confidence: 99%

“…JBTX was found to physically interact with and to increase the activity of UDP-N-acetylglucosamine pyrophosphorylase (UDP-GlcNAcP), an enzyme involved in glycosylation pathways and in the synthesis of chitin [22]. …”

Section: What’s New?mentioning

confidence: 99%

“…In an in vitro study, JBTX also activated UDP-GlcNAcP from the cotton stainer bug Dysdercus peruvianus , an insect that is susceptible to the insecticidal activity of both, urease and JBTX [22, 23]. …”

Section: What’s New?mentioning

confidence: 99%

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Jaburetox: update on a urease-derived peptide

Becker-Ritt

Portugal

Carlini

2017

J Venom Anim Toxins Incl Trop Dis

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Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes. A recombinant form of this peptide, called jaburetox (JBTX), was constructed using jbureII gene as a template. The peptide exhibits liposome disruption properties, and insecticidal and fungicidal activities. Here we review the known biological properties activities of JBTX, and comment on new ones not yet fully characterized. JBTX was able to cause mortality of Aedes aegypti larvae in a feeding assay whereas in a dose as low as of 0.1 μg it provoked death of Triatoma infestans bugs. JBTX (10−5–10−6 M) inhibits the growth of E. coli, P. aeruginosa and B. cereus after 24 h incubation. Multilamellar liposomes interacting with JBTX undergo reorganization of the membrane’s lipids as detected by small angle X-ray scattering (SAXS) studies. Encapsulating JBTX into lipid nanoparticles led to an increase of the peptide’s antifungal activity. Transgenic tobacco and sugarcane plants expressing the insecticidal peptide JBTX, showed increased resistance to attack of the insect pests Spodoptera frugiperda, Diatraea saccharalis and Telchin licus licus. Many questions remain unanswered; however, so far, JBTX has shown to be a versatile peptide that can be used against various insect and fungus species, and in new bacterial control strategies.

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JBTX strongly inhibited the activity of nitric oxide synthase, thereby reducing the levels of the nitric oxide neurotransmitter;

…”

Section: What’s New?mentioning

confidence: 99%

Section: What’s New?mentioning

confidence: 99%

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Jaburetox: update on a urease-derived peptide

Becker-Ritt

Portugal

Carlini

2017

J Venom Anim Toxins Incl Trop Dis

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“…Previous data from our group demonstrated that the central nervous system (CNS) of the triatomines Triatoma infestans and R. prolixus is a target organ for Jbtx, and a recent work reported that Jbtx interacts with the CNS of N. cinerea upon in vitro incubation (Broll et al, 2017). Using an immunoproteomic approach with the CNS of T. infestans , Galvani et al (2015) demonstrated an interaction between Jbtx and the enzyme UDP‐ N ‐acetylglucosamine pyrophosphorylase (UAP). This enzyme produces the sugar nucleotide UDP‐ N ‐acetylglucosamine (UDP‐GlcNAc), essential for chitin synthesis, protein glycosylation, and production of glycophosphatidylinositol (GPI) anchors (Merzendorfer, 2011; Moussian, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…This enzyme produces the sugar nucleotide UDP‐ N ‐acetylglucosamine (UDP‐GlcNAc), essential for chitin synthesis, protein glycosylation, and production of glycophosphatidylinositol (GPI) anchors (Merzendorfer, 2011; Moussian, 2008). Similarly, when Jbtx was injected in T. infestans , the peptide was detected at the antennal lobe and the subesophageal ganglion, sites where nitric oxide synthase (NOS) is present (Galvani et al, 2015). This enzyme is involved in nitrinergic signaling, immune response, sense of location and search for food (Muller, 1997; Stefano & Ottaviani, 2002).…”

Section: Introductionmentioning

confidence: 99%

Jaburetox, a urease‐derived peptide: Effects on enzymatic pathways of the cockroach Nauphoeta cinerea

Perin

Noronha

Moyetta

et al. 2020

Arch Insect Biochem Physiol

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Jaburetox is a recombinant peptide derived from one of the Canavalia ensiformis urease isoforms. This peptide induces several toxic effects on insects of different orders, including interference on muscle contractility in cockroaches, modulation of UDP‐N‐acetylglucosamine pyrophosphorylase (UAP) and nitric oxide synthase (NOS) activities in the central nervous system of triatomines, as well as activation of the immune system in Rhodnius prolixus. When injected, the peptide is lethal for R. prolixus and Triatoma infestans. Here, we evaluated Jaburetox toxicity to Nauphoeta cinerea cockroaches, exploring the effects on the central nervous system through the activities of UAP, NOS, acid phosphatases (ACP), and acetylcholinesterase (AChE). The results indicated that N. cinerea is not susceptible to the lethal effect of the peptide. Moreover, both in vivo and in vitro treatments with Jaburetox inhibited NOS activity, without modifying the protein levels. No alterations on ACP activity were observed. In addition, the enzyme activity of UAP only had its activity affected at 18 hr after injection. The peptide increased the AChE activity, suggesting a mechanism involved in overcoming the toxic effects. In conclusion, our findings indicate that Jaburetox affects the nitrinergic signaling as well as the AChE and UAP activities and establishes N. cinerea as a Jaburetox‐resistant model for future comparative studies.

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