Effect of the stage of estrous cycle on follicular population, oocyte yield and quality, and biochemical composition of serum and follicular fluid in Anatolian water buffalo

Acar, Duygu Baki; Birdane, Muhammed Kürşad; Doğan, Nurhan; Gürler, Hande

doi:10.1016/j.anireprosci.2012.12.004

Cited by 14 publications

(20 citation statements)

References 42 publications

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“…The average number of follicles obtained per cow (13.9± 3.44) was comparable to that reported by Carvalho et al [33] in Nellore zebu (16.7 ± 1.6) and Kouamo et al [14] in Gudali Cameroonian zebu (16.75 ± 0.8), but lower than the number reported by Fassi et al [16] (22.98± 8.41) in Morocco zebu. Meanwhile, the mean numbers of medium and large follicles were similar to the findings of Acar et al [34] and Kouamo et al [35] on different breed of Cameroonian zebu (Gudali, Bokolo). These fluctuations may be related either to breed [36,37] or to the livestock's management.…”

supporting

confidence: 89%

Effects of Breed, Age, Body Condition Score, and Nutritional Status on Follicular Population, Oocyte Yield, and Quality in Three Cameroonian Zebus CattleBos indicus

Dorice

Ferdinand

Kouamo

et al. 2019

Advances in Agriculture

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This study was aimed at investigating the effects of breed, age, body condition score, and nutritional status on follicular dynamics, quality, and oocyte yield in Cameroonian zebus cattle Bos indicus (Gudali and Red and White Fulani). A total of 95 cows were investigated for breed, age, body condition score, and nutritional status. Blood samples and ovaries of these cows were collected during slaughtering. Follicles on each ovary were counted and measured using electronic digital calipers and then classified into three categories: small (<3 mm), medium (3 – 8 mm), and large (> 8 mm) diameter. The oocytes were retrieved by slicing each ovary into a Petri dish containing collected medium. The oocytes recovered were examined under a stereoscope (x 10) and classified into four groups based on the morphology of cumulus oophorus cells and cytoplasmic changes. From these ovaries, 3888 follicles were counted with an average population of 27.81 ± 6.88 follicles per cow. The average numbers of small, medium, and large follicles per cow were 17.26 ± 1.36 (62.06%), 9.64 ± 1.00 (34.66%), and 0.52 ± 0.32 (1.86%), respectively. A total of 1822 oocytes were harvested with an average of 19.11 ± 0.89 oocytes per cow. Oocytes graded I, II, III, and IV were 7.03 ± 2.30 (36.65%), 4.53± 2.01 (23.61%), 3.19 ± 0.85 (16.63%), and 4.43 ±1.62 (23.09%), respectively. This study also showed that ovarian weight, number of follicles, and oocytes’ yield of right ovary were higher than left ones (P < 0.05). Younger and normal cows (BCS = 3) presented higher (P < 0.05) number of follicles and oocytes. Urea and cholesterol levels were higher in Gudali and White Fulani cows (P < 0.05). This study indicated that ovaries of zebus harvested in slaughterhouse are an important source of follicular population, oocytes yield, and quality. Right ovaries are heavy and offer more numbers of follicles and oocytes than the left ones. BCS is the best indicator of nutritional status of cows. Important follicle populations are obtained from cows aging 6-9 years with BCS = 3.

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supporting

confidence: 89%

Effects of Breed, Age, Body Condition Score, and Nutritional Status on Follicular Population, Oocyte Yield, and Quality in Three Cameroonian Zebus CattleBos indicus

Dorice

Ferdinand

Kouamo

et al. 2019

Advances in Agriculture

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“…The average number of follicles counted per cow was higher than 16.75±1.05 and 32 reported by Acar et al . [ 9 ] and Takaji et al . [ 14 ], respectively.…”

Section: Discussionmentioning

confidence: 99%

“…All subsequent experimental procedures were conducted in laminar flow. The apparent follicles on each ovary were measured using an electronic caliper then counted and classified according to their diameter into small (<3 mm), medium (3-8 mm), and large (>8 mm) follicles [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of nutritional status on the ovarian follicular population, yield and quality of oocytes in the Ngaoundere Gudali zebu (Bos indicus)

Kouamo¹,

Tidjou²,

Zoli³

et al. 2015

Vet World

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Aim:The aim of this study was to investigate the effect of nutritional status of the Gudali cows slaughtered at the Ngaoundere abattoir on follicular population, quality, and oocytes yield.Materials and Methods:Blood and ovaries were collected from 81 cows aged 6.35±0.24 years (3-12 years old), with a body condition score (BCS) of 2.93±0.09 (1-5). In each ovary, the follicle were counted and classified as small (<3 mm), medium (3-8 mm) and large (>8 mm) using an electronic caliper. Oocytes were collected by slicing technique and classified according to the homogeneity of the cytoplasm and layers of granulosa into four groups: I, II, III, and IV. The nutritional status of the animals was determined by quantification of serum glucose, total cholesterol, total protein, albumin, globulins, urea, and phosphorus level.Results:Of the total 162 ovaries harvested, 2916 follicles were counted on the ovarian surface with an average population of 36.00±2.17 follicles/cow. According to a size distribution, 16.67±1.54 (46.3%), 18.83±1.27 (52.3%), and 0.51±0.07 (1.4%), respectively for small (<3 mm), medium (3-8 mm), and large (>8 mm) were recorded. About 1,929 oocytes were obtained, with an average recovered of 23.81±1.53 oocytes/cow. Depending on the quality, 7.79±0.55 (32.7%), 6.04±0.41 (25.3%), 4.89±0.44 (20.6%), and 5.10±0.54 (21.4%) oocytes qualities I, II, III, and IV were obtained respectively; with an average cultivable oocyte recovered of 13.83±0.89 (58%). Cows with BCS > 3 and a high albumin and phosphorus level showed a highest number of follicles and oocytes able for in vitro maturation.Conclusion:These results indicated that nutrition remains an important factor for the in vitro production of the good embryo and the BCS is a useful tool for the selection of females’ oocytes donors.

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“…For each ovary, visible follicles were counted and follicular diameters (Φs) measured with electronic Stainless Hardened calipers. Follicular diameters were classified into three categories as described by Duygu et al (2013).…”

Section: Follicular Population Determinationmentioning

confidence: 99%

“…Oocyte quality was evaluated under a stereoscope (× 10) and scored into four grades (G) according to the homogeneity of the cytoplasm and layers of cumulus cells (Figure 1) as described by Alves et al (2014). The overall oocyte quality per cow was calculated as an index using the formula by Duygu et al (2013): (G I × 1 + G II × 2 + G III × 3 + G IV × 4) / Total number of oocytes recovered.…”

Section: Recovery and Grading Of Oocytesmentioning

confidence: 99%

Evaluation de la population folliculaire et de la qualité des ovocytes des vaches zébus (<em>Bos indicus</em>) en anœstrus anovulatoire

Kouamo

Zangue

Zoli

2019

Rev. Elev. Med. Vet. Pays Trop.

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L’étude, menée à l’abattoir municipal de Ngaoundéré, région de l’Adamaoua au Cameroun, visait à évaluer la population folliculaire et la qualité des ovocytes des vaches zébus locales en anœstrus anovulatoire. Sur 496 voies génitales examinées, 145 (29,2 %) présentaient un anœstrus anovulatoire. Le nombre moyen de follicules était de 37,5 ± 25,2 par vache. Le nombre de petits, moyens et grands follicules par vache était respectivement de 21,7 ± 18,7, 14,9 ± 12,1 et 0,9 ± 1,1. Les populations folliculaires des vaches cycliques ou en anœstrus anovulatoire étaient respectivement de 29,1 ± 18,1 et 41,9 ± 27,3 (p < 0,05). Le nombre d’ovocytes par vache était respectivement de 15,5 ± 4,5 et 15,6 ± 3,8 (p > 0,05) chez les vaches cycliques et en anœstrus anovulatoire. Le nombre d’ovocytes de grades I, II, III et IV était respectivement de 4,5 ± 4,1 (28,5 %), 4,1 ± 3,7 (26,2 %), 2,3 ± 2,4 (14,8 %) et 4,8 ± 5,6 (30,5 %). Cette étude a montré que l’anœstrus anovulatoire avait tendance à augmenter la population folliculaire mais n’avait aucun effet sur le nombre d’ovocytes récoltés. Le nombre d’ovocytes de grades I et II utilisables pour la maturation in vitro était significativement plus faible chez les vaches en anœstrus anovulatoire que chez les vaches cycliques (p < 0,05).

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Effect of the stage of estrous cycle on follicular population, oocyte yield and quality, and biochemical composition of serum and follicular fluid in Anatolian water buffalo

Cited by 14 publications

References 42 publications

Effects of Breed, Age, Body Condition Score, and Nutritional Status on Follicular Population, Oocyte Yield, and Quality in Three Cameroonian Zebus CattleBos indicus

Effects of Breed, Age, Body Condition Score, and Nutritional Status on Follicular Population, Oocyte Yield, and Quality in Three Cameroonian Zebus CattleBos indicus

Effect of nutritional status on the ovarian follicular population, yield and quality of oocytes in the Ngaoundere Gudali zebu (Bos indicus)

Evaluation de la population folliculaire et de la qualité des ovocytes des vaches zébus (<em>Bos indicus</em>) en anœstrus anovulatoire

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