Effect of the silk protein sericin on cryopreserved rat islets

Ohnishi, Koji; Murakami, Makoto; Morikawa, Mitsuhiro; Yamaguchi, Akio

doi:10.1007/s00534-011-0415-4

Cited by 42 publications

(28 citation statements)

References 19 publications

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“…Most studies to date have not found sericin superior to FBS in cryopreservative experiments. 21,22,33 One exception where a freezing medium containing sericin performed better than a medium with FBS was a study using Chinese-hamster ovary cells frozen in PBS, supplemented with 1% sericin, 0.5% maltose, 0.3% proline, 0.3% glutamine, and 10% DMSO. 18 Thus, our results are in agreement with the majority of data published thus far.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Sericin as a Fetal Bovine Serum-Replacing Cryoprotectant During Freezing of Human Mesenchymal Stromal Cells and Human Osteoblast-Like Cells

Verdanova

Pytlik

Kalbáčová

2014

Biopreservation and Biobanking

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A reliable, cryoprotective, xeno-free medium suitable for different cell types is highly desirable in regenerative medicine. There is danger of infection or allergic reaction with the use of fetal bovine serum (FBS), making it problematic for medical applications. The aim of the present study was to develop an FBS-free cryoprotective medium for human mesenchymal stromal cells (hMSCs; primary cells) and immortalized human osteoblasts (SAOS-2 cell line). Furthermore, we endeavored to eliminate or reduce the presence of dimethyl sulfoxide (DMSO) in the medium. Sericin, a sticky protein derived from the silkworm cocoon, was investigated as a substitute for FBS and DMSO in the freezing medium. Cell viability (24 hours after thawing, both hMSC and SAOS-2) and colony-forming ability (2 weeks after thawing, only for hMSCs) were both determined. The FBSfree medium with 1% sericin in 10% DMSO was found to be a suitable freezing medium for primary hMSCs, in contrast to immortalized human osteoblasts. Surprisingly, the storage of hMSCs in a cultivation medium with only 10% DMSO also provided satisfactory results. Any drop in DMSO concentration led to significantly worse survival of cells, with little improvement in hMSC survival in the presence of sericin. Thus, sericin may substitute for FBS in the freezing medium for primary hMSCs, but cannot substitute for DMSO.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Sericin as a Fetal Bovine Serum-Replacing Cryoprotectant During Freezing of Human Mesenchymal Stromal Cells and Human Osteoblast-Like Cells

Verdanova

Pytlik

Kalbáčová

2014

Biopreservation and Biobanking

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“…It has successfully improved the survival of various cell cultures during cryopreservation when used as a protein in the freezing medium (Terada et al 2002;Sasaki et al 2005). As proteins have a lower toxicity than DMSO and are also thought to act as a cell membrane-impermeable cryoprotectant and have an inhibitory effect against the ice crystals formation, sericin as a cell membrane-impermeable cryoprotectant may thus protect cells from cryo-injury (Ohnishi et al 2011).…”

Section: Sds-polyacrylamide Gel Electrophoresis (Sds-page)mentioning

confidence: 99%

Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering

2012

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Silk sericin protein is a natural, hydrophilic, macromolecular glycoprotein mainly synthesized in the middle silk gland of the silkworm. It constitutes 25-30% of the silk cocoon. Sericin proteins have antioxidant, antimicrobial, UV-resistant properties, promote wound healing and support cell proliferation even in serum-free media. Most of the sericin is discarded as waste in silk processing industries. This study aims at improving the mechanical strength and stability of sericin extracted from the silk cocoons during processing and utilize it as a biocompatible natural biopolymer in biomedical applications. Crosslinked sericin membranes, from the cocoon of non-mulberry tropical silkworm, Antheraea mylitta, were prepared using gluteraldehyde as the crosslinking agent. Physical and structural characteristics of the membranes were analyzed using scanning electron microscopy, atomic force microscopy, Fourier transform infrared spectroscopy and X-ray diffraction along with swelling and degradation studies. The secondary structure of the membrane indicates that crosslinking provides a more integrated structure that significantly improves the stability and mechanical strength of the membranes. In vitro cytocompatibility of the membranes was evaluated by MTT assay and cell cycle analysis of feline fibroblast cells. The adherence, growth and proliferation patterns of cells on membranes were assessed by confocal microscopy, which demonstrated that the latter is non-toxic and supports cell growth. Cell cycle analyses indicate cytocompatibility with normal cell cycle pattern. This study reveals that silk sericin protein can be used as a biocompatible natural biopolymer for various applications in the biomedical field.

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“…Among its wide range of physiological functions, sericin has been reported to ameliorate constipation (Sasaki et al, 2000a), suppress colorectal cancer (Sasaki et al, 2000b;Zhaorigetu et al, 2001;Zhaorigetu et al, 2007), suppress skin cancer (Zhaorigetu et al, 2003b), and apply cell activating/cytoprotective properties (Terada et al, 2002;Terada et al, 2005;Ohnishi et al, 2012) and adiponectin-increasing/promoting effects (Okazaki et al, 2010). In addition, supplements exerting physiological effects have been put to practical use.…”

Section: Introductionmentioning

confidence: 99%

Development of Bread Supplemented with the Silk Protein Sericin

Takechi

Takamura

2014

FSTR

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The present study aimed to develop sericin-added bread in an effort to promote the effective consumption of the silk protein sericin and to evaluate bread-baking performance with sericin addition, with respect to effects on bread structure and palatability. Sericin supplementation resulted in decreased specific volume and darker crust color of the bread. No significant difference was observed in other physical properties. Sensory evaluation revealed that bread with up to 2 g of added sericin tended to be preferred. In a comprehensive evaluation of specific volume, crust color and preference rating by sensory evaluation, the optimal amount of added sericin was determined to be 2 g.Further, approximately 330 mg of sericin can be consumed in 1 slice of bread. We thus propose sericin-added bread as a desirable processed food that enables the effective ingestion of sericin.

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Effect of the silk protein sericin on cryopreserved rat islets

Abstract: The present results showed that serum-free medium containing sericin is useful for both cryopreservation and cell culture.

Cited by 42 publications

References 19 publications

Evaluation of Sericin as a Fetal Bovine Serum-Replacing Cryoprotectant During Freezing of Human Mesenchymal Stromal Cells and Human Osteoblast-Like Cells

Evaluation of Sericin as a Fetal Bovine Serum-Replacing Cryoprotectant During Freezing of Human Mesenchymal Stromal Cells and Human Osteoblast-Like Cells

Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering

Development of Bread Supplemented with the Silk Protein Sericin

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