Effect of the Samwondan Ethanol Extract on Obesity Inducer

Abstract

Objectives : In this study, we investigated the biological activities such as anti-obesity using Samwondan ethanol extract (SWD). SWD is a complex with Salicornia herbacea Linnaeus, Saururus chinensis Baill and Houttuynia cordata Thunberg as the main raw material. Methods : The SWD was extracted 80% ethanol. 3T3-L1 preadipocytes were induced adipogenesis by differentiation media with SWD at 1 µg/mL, 10 µg/mL, and 100 µg/mL. Effect of SWD performed using MTT assay, oil red O staining (observation by microscope), and reverse transcription polymerase chain reaction. Also we measured production of triglyceride (TG), fatty acid, and acetyl-CoA carboxylase (ACC). Results : Non-cytotoxicity was in all test group from range of 1 µg/mL to 100 µg/mL on pre-adipocyte. The droplet and production of lipid were decreased significantly by the SWD. And TG was decreased by approximately 89%, 85% and 82%, upon the SWD treatment at concentration of 1 µg/mL, 10 µg/mL, and 100 µg/mL. Moreover, the SWD showed inhibitory effects on the expression of the C/EBP (CCAAT/enhaner binding protein)β , C/EBPα , and peroxisome proliferator-activated receptor γ genes in adipocytes. The SWD at 100 µg/mL concentration showed inhibitory effect on fatty acid production by 79%. Also ACC production were decreased dose-dependently. Conclusions : From the results above, we concluded that the SWD indicated significantly anti-obesity effects.

The Effect of Herbal-acupuncture at KI on Lipopolysaccharide Induced Nephritis in Rats

“…한의학의 치료법 중 현대에 들어와서 새롭게 생긴 藥鍼 시술을 통하여 穴位의 치료효과와 藥物의 치료효과를 동시 에 얻을 수 있다. 본 연구에서 穴位는 足少陰腎經 중 合穴이 면서 本經의 속성인 水의 속성을 가진 陰谷 [11][12][13] 을, 약물은 利水滲濕 작용이 있다고 알려진 白茯苓 [14][15][16] 을 선택하여 신 장염의 치료효과에 대한 연구를 진행하였다.…”