Effect of the route of administration on the mucosal and systemic immune responses to Lawsonia intracellularis vaccine in pigs

Abstract

In an on-farm study, 40 weaned piglets aged 3 weeks were vaccinated with Lawsonia intracellularis vaccine orally, IM or IP while a fourth group remained unvaccinated. All vaccinated animals showed increased serum levels of L. intracellularis-specific IgG antibodies, but significantly elevated concentrations of specific IgG, IgA and cytokines were generated in ileal mucosal secretions from the orally and IP vaccinated pigs when examined at 17 days after vaccination.

Community composition and development of the post-weaning piglet gut microbiome

“…A number of non-antibiotic strategies to increase resistance to disease have been studied 49 . Strategies for which bene cial effects have been reported consist of hydrolases 50 , ber intake 51 , β-lactamase enzymes 52 , non-resistance inducing antimicrobial drugs 53,54 , vaccination [55][56][57][58] , phage therapy 59 , in-feed organic acids 60 , starches 61 , and liquid feed [62][63][64] . Extensive evidence exists on the use of probiotics.…”

Systemic cytokine response in pigs infected orally with a <i>Lawsonia intracellularis</i> isolate of South Korean origin

“…In the swine industry, L. intracellularis is one of the main enteric pathogens; it causes acute intestinal hemorrhage (proliferative hemorrhagic enteropathy, PHE) in naïve adult pigs and a wasting disease (PE) in growing pigs [ 8 , 13 ]. Variations in microbial virulence, the spectrum of symptoms, and antibody responses have been described for L. intracellularis infection or vaccination [ 1 , 16 , 17 , 19 ].…”

“…Among various cytokines, interferon-γ (IFN-γ) has been reported to play a significant role in limiting intracellular infection and increasing cellular proliferation associated with L. intracellularis and so far most immunological researches on L. intracellularis infection have focused mainly on IFN-γ [ 2 , 3 , 20 , 22 ]. The relevance of IFN-γ in L. intracellularis infection has been assessed mainly using IFN-γ receptor knockout mice [ 2 , 22 ], normal pigs [ 3 , 16 , 17 , 20 , 21 ], and horses [ 18 ].…”

In this issue – April 2015

“…A study conducted in a commercial piggery in Victoria measured mucosal and systemic immune responses to attenuated L. intracellularis vaccine delivered by oral, intramuscular (IM) and intraperitoneal (IP) routes. 7 All vaccinated animals showed increased serum levels of L. intracellularis-specific IgG antibodies at 17 days post vaccination. Mucosal IgG and IgA antibodies were significantly increased following oral and IP…”