Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis

Vílchez, M. Carmen; Santangeli, Stefania; Maradonna, Francesca; Gioacchini, Giorgia; Verdenelli, Cristina; Gallego, Víctor; Peñaranda, David S.; Tveiten, Helge; Pérez, Luz; Carnevali, Oliana; Asturiano, Juan F.

doi:10.1016/j.theriogenology.2015.07.011

Cited by 34 publications

(19 citation statements)

References 55 publications

(91 reference statements)

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“…The function of bmp15 in the testis, on the other hand, is not well established. bmp15l is expressed in the salmon testis/germ cells, as well as in the testis of several species (Clelland et al, ; Chen et al, ; Zhang et al, ; Vilchez et al, ). Interestingly, an association between mutation in the BMP15 gene and sperm quality was recently reported for Chinese Holstein bull, and the authors of this study suggest that BMP15 may regulate testicular function, given its high expression in the bull testis (Sun et al, ).…”

Section: Discussionmentioning

confidence: 99%

bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.)

Kleppe

Edvardsen

Furmanek

et al. 2016

Molecular Reproduction Devel

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Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad-specific genes using available adult multi-tissue transcriptomes. We excluded genes with expression in tissues other than gonads based on quantity of reads, and then a subset of genes was selected for verification in a multi-tissue PCR screen. Four gonad-specific genes (bmp15l, figla, smc1bl, and larp6l) were chosen for further characterization, namely: germ cell specificity, investigated by comparing mRNA abundance in wild-type and germ cell-free gonads by quantitative real-time PCR, and cellular location, visualized by in situ hybridization. All four genes were expressed in both testis and ovary, and preferentially within the germ cells of both sexes. These genes may be essential players in salmon germ cell development, and could be important for future studies aiming to understand and control reproduction. Mol. Reprod. Dev. 84: 76-87, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.)

Kleppe

Edvardsen

Furmanek

et al. 2016

Molecular Reproduction Devel

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“…In a study using European eel ( Anguilla anguilla ), Vílchez et al ( 2015 ) administered three dose (10 3 ,10 5 , and 10 6 CFU/g) of Lb. rhamnosus in the diet and monitored possible effects on spermatogenesis process.…”

Section: Lab As Probioticmentioning

confidence: 99%

Lactic Acid Bacteria in Finfish—An Update

et al. 2018

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A complex and dynamic community of microorganisms, play important roles within the fish gastrointestinal (GI) tract. Of the bacteria colonizing the GI tract, are lactic acid bacteria (LAB) generally considered as favorable microorganism due to their abilities to stimulating host GI development, digestive function, mucosal tolerance, stimulating immune response, and improved disease resistance. In early finfish studies, were culture-dependent methods used to enumerate bacterial population levels within the GI tract. However, due to limitations by using culture methods, culture-independent techniques have been used during the last decade. These investigations have revealed the presence of Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Streptococcus, Carnobacterium, Weissella, and Pediococcus as indigenous species. Numerous strains of LAB isolated from finfish are able to produce antibacterial substances toward different potential fish pathogenic bacteria as well as human pathogens. LAB are revealed be the most promising bacterial genera as probiotic in aquaculture. During the decade numerous investigations are performed on evaluation of probiotic properties of different genus and species of LAB. Except limited contradictory reports, most of administered strains displayed beneficial effects on both, growth—and reproductive performance, immune responses and disease resistance of finfish. This eventually led to industrial scale up and introduction LAB-based commercial probiotics. Pathogenic LAB belonging to the genera Streptococcus, Enterococcus, Lactobacillus, Carnobacterium, and Lactococcus have been detected from ascites, kidney, liver, heart, and spleen of several finfish species. These pathogenic bacteria will be addressed in present review which includes their impacts on finfish aquaculture, possible routes for treatment. Finfish share many common structures and functions of the immune system with warm-blooded animals, although apparent differences exist. This similarity in the immune system may result in many shared LAB effects between finfish and land animals. LAB-fed fish show an increase in innate immune activities leading to disease resistances: neutrophil activity, lysozyme secretion, phagocytosis, and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α). However, some LAB strains preferentially induces IL-10 instead, a potent anti-inflammatory cytokine. These results indicate that LAB may vary in their immunological effects depending on the species and hosts. So far, the immunological studies using LAB have been focused on their effects on innate immunity. However, these studies need to be further extended by investigating their involvement in the modulation of adaptive immunity. The present review paper focuses on recent findings in the field of isolation and detection of LAB, their administration as probiotic in aquaculture and their interaction with fish immune responses. Furthermore, the mode of action of probiotics on finfish are discussed.

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“…Normal spermatogenesis is essential for reproduction and depends upon the regulation of gene expression [ 4 , 5 , 6 ]. Studies have been performed at molecular and epigenetic levels to elucidate the molecular basis of spermatogenesis and the specific role of these molecules in the function of spermatozoa [ 7 , 8 , 9 , 10 ]. Several master genes, such as cyclic AMP-responsive element modulator ( CREM ) [ 11 ], sperm-associated antigen 6 ( Spag6 ) [ 12 ], sperm-associated antigen 6 ( Spag16 ) [ 13 ], phosphoglycerate kinase 2 ( Pgk2 ) [ 14 ], have been identified to be involved in spermatogenesis and affect the functions of spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

miR-34a Regulates Sperm Motility in Zebrafish

Guo

Xie

Xiong

et al. 2017

IJMS

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Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system. miR-34a knockout zebrafish showed no obvious defects on testis morphology and sperm quantity. However, we found a significant increase in progressive sperm motility that is one of the pivotal factors influencing in vitro fertilization rates, in the knockout zebrafish. Moreover, breeding experiments showed that, when miR-34a-knockout male zebrafish mated with the wide-type females, they had a higher fertilization rate than did the wide-type males. Glycogen synthase kinase-3a (gsk3a), a potential sperm motility regulatory gene was predicted to be targeted by miR-34a, which was further supported by luciferase reporter assays, since a significant decrease of luciferase activity was detected upon ectopic overexpression of miR-34a. Our findings suggest that miR-34a downregulates gsk3a by targeting its 3′ untranslated region, and miR-34a/gsk3a interaction modulates sperm motility in zebrafish. This study will help in understanding in the role of the miR-34 family during spermatogenesis and will set paths for further studies.

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Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis

Cited by 34 publications

References 55 publications

bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.)

bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon (Salmo salar L.)

Lactic Acid Bacteria in Finfish—An Update

miR-34a Regulates Sperm Motility in Zebrafish

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