Effect of the Pro12Ala polymorphism of the peroxisome proliferator-activated receptor gamma-2 gene on adiposity, insulin sensitivity and lipid profile in the Spanish population

Abstract

Objective: To investigate the role of the Pro12Ala peroxisome proliferator-activated receptor (PPAR) g-2 polymorphism in the susceptibility to the insulin resistance syndrome and its metabolic complications in a population-based nationwide multicenter study in Spain. Design: 464 unrelated adults (45.3% men and 54.7% women) aged between 35 and 64 years were randomly chosen from a nationwide population-based survey of obesity and related conditions including insulin resistance and cardiovascular risk factors. Methods: Anthropometric determinations included: body mass index (BMI), waist-to-hip ratio, sagittal abdominal diameter; biochemical determinations included: fasting plasma glucose concentration and concentration 2 h after an oral glucose tolerance test (OGTT), total cholesterol, high and low density lipoprotein-cholesterol, triglycerides, leptin and insulin. Systolic and diastolic blood pressure were also measured. Genotyping of the PPARg-2 Pro12Ala polymorphism was determined by polymerase chain reaction and single strand conformation polymorphism analysis. Results: The Ala12 allele frequency was higher in obese men than in lean men (0.15 vs 0.08, P ¼ 0:03). Men carriers of the Ala12 allele had a higher BMI than non-carriers (38.9% vs 21.3%; adjusted odds ratio 2.36, 95% confidence interval 1.10 -5.05, P ¼ 0:03). However, despite higher BMI obese men carriers of the Ala12 allele had lower sagittal abdominal diameter than Pro12 homozygotes ð24:1^3:2 vs 26:3^2:5 cm; P ¼ 0:01Þ: The Ala12 allele was associated with lower total triglycerides levels in the overall population and it was also associated with lower fasting insulin levels and a higher insulin sensitivity by homeostasis model assessment (HOMA) in women. Conclusions: Our results suggest that the Pro12Ala polymorphism of the PPARg-2 gene promotes peripheral deposition of adipose tissue and increased insulin sensitivity for a given BMI. The results in women might be due to their different adipose tissue distribution.

Association between Pro12Ala, Pvull, Avall, Sstl and ADIPOQ Single-Nucleotide Polymorphisms with Lipid and Glycemic Profiles of Patients with Berardinelli-Seip Syndrome

“…In our sample of BSS cases, we found the Pro12Ala polymorphism in 13.7% and the Ala12 allele in 6.85%, findings that are compatible with Caucasian samples in different countries, as follows: 12.6% in Danes [23], 15.38% in American Caucasians [18], 12% in Italians [24] and 16.1% in Spaniards [20]. However, our results were slightly lower than those obtained for Finns (27.33%) [19] and Swedes (32.39%) [25].…”

“…Similarly, Spanish women with the Ala12 allele are more sensitive to insulin and have better lipid profiles than those with the Pro12Pro genotype and a lower prevalence of DM2. These findings suggest that the Pro12Ala polymorphism promotes decreased peripheral deposition of adipose tissue and increased insulin sensitivity [20]. …”

Obesity and Pro12Ala Polymorphism of Peroxisome Proliferator-Activated Receptor-Gamma Gene in Healthy Adults: A Systematic Review and Meta-Analysis

“…Nine cohort studies [12,25,26,27,28,29,30,31,32], 7 population case-control studies [33,34,35,36,37,38,39], 31 cross-sectional studies [5,7,10,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67], and 9 interventional studies [8,68,69,70,71,72,73,74,75] were included in the meta-analysis of the association between PPARγ Pro12Ala polymorphism and obesity. The characteristics and results of the studies are summarized in table 1.…”

“…The characteristics and results of the studies are summarized in table 1. Seven population case-control studies [33,34,35,36,37,38,39], and 1 cross-sectional study [10] provided their data in 2 datasets. Each dataset was treated as a separate comparison in the meta-analysis.…”

PPAR gamma pro12Ala polymorphism and type 2 diabetes: a study in a spanish cohort

“…Furthermore, the presence of other doi: 10.7243/2054-1112-2-1 vascular risk factors has also been explored in relation to the association between PPAR-γ and the onset of T2D. Similarly, other studies have demonstrated the PPAR-γ/T2D onset in association with Body Mass Index lipid profiles, also extending studies to secondary organ damage resulting from diabetes, diabetic nephropathy and coronary heart disease [19][20][21][22].…”