Effect of the Presence of Glucose during Fertilization and/or Culture in a Chemically Semi-Defined Medium on the Development of Bovine Oocytes Matured and Fertilized in Vitro.

Lim, Jeeyoung; Kim, Jong-Heung; Okuda, Kiyoshi; Niwa, K.

doi:10.1262/jrd.39.237

Cited by 9 publications

(4 citation statements)

References 18 publications

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“…Other reports (Niwa and Ohgoda, 1988;Shioya et al, 1988), showed that in a medium with caffeine or caffeine plus heparin, bovine spermatozoa can be capacitated, and penetrate in the presence of glucose. Finally, irrespective of the presence of glucose, a high penetration rate was obtained in a medium containing caffeine and heparin (Lim et al, 1993;Tajik et al, 1998). Our results confirm that the presence of glucose does not affect penetration by bovine spermatozoa in a medium containing caffeine and heparin or in a medium without bicarbonate.…”

Section: Experiments 5-effect Of Glucose On Sperm Penetrationsupporting

confidence: 79%

“…Although glucose does not affect fertilization, the presence of glucose during fertilization may be needed for normal development to the blastocyst stage. However, it has been reported that the presence of glucose in the fertilization medium inhibited development to the morula and blastocyst stages (Lim et al, 1993). This discrepancy may arise from the different media for fertilization used in the two studies.…”

Section: Experiments 6-developmental Capacity Of Penetrated Oocytesmentioning

confidence: 94%

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Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium

Park¹,

Niwa²

2009

Asian Australas. J. Anim. Sci

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A modified Tris-buffered medium (mTBM) has been widely used as an insemination medium for porcine in vitro fertilization (IVF). We examined whether mTBM could be used for bovine IVF. Bovine cumulus-oocyte complexes (COCs) were cultured in a serum-free medium containing 30 ng/ml EGF for 22 h. After culture, COCs were inseminated with spermatozoa for 12 h in mTBM containing 5 mM caffeine and 10 g/ml heparin. The penetration of oocytes increased significantly (p<0.05) as the sperm concentration increased from 0.1 (30%) to 1-10 (87-100%)×10 6 cells/ml. This was significantly different from values obtained at 1 (87%) and 10 (100%)×10 6 cells/ml. However, when COCs were inseminated with spermatozoa from different bulls, the proportions (62-100%) of oocytes penetrated varied according to the bull. The proportion (18%) of oocytes penetrated was significantly (p<0.05) lower in a fertilization medium without caffeine and heparin but increased with the addition of caffeine and/or heparin to the medium, and the proportion (93-96%) of oocytes penetrated increased significantly (p<0.05) when the medium was supplemented with heparin and caffeine. In this medium, sperm penetration was first observed at 3 h after insemination. Irrespective of the presence of glucose in the fertilization medium, the proportion (93-97%) of oocytes penetrated and the proportion (83-84%) of embryos at the ≥2-cell stage cultured in a chemically defined medium were not significantly different. However, the proportion of embryos developing to the blastocyst stage was significantly (p<0.05) higher in the presence (11%) of glucose in the fertilization medium than in its absence (2%).In conclusion, the present study demonstrated that bovine oocytes penetrated in vitro in mTBM can develop to the blastocyst stage and mTBM may be used for the in vitro production of bovine embryos.

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Section: Experiments 5-effect Of Glucose On Sperm Penetrationsupporting

confidence: 79%

Section: Experiments 6-developmental Capacity Of Penetrated Oocytesmentioning

confidence: 94%

Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium

Park¹,

Niwa²

2009

Asian Australas. J. Anim. Sci

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“…In particular, glucose at concentrations ranging from 1.5t o 5.56 mM, has been reported to improve blastocyst production both in presence of Ž serum Rorie et al, 1994;Carolan et al, 1995;Koji et al, 1997;Wang et al, 1997; . Ž Donnay et al, 1999 and in the presence of BSA Kim et al, 1993a;Lim et al, 1993Lim et al, , . 1994aOhboshi et al, 1996;Furnus et al, 1997 .…”

Section: Introductionmentioning

confidence: 99%

Effects of glucose and protein sources on bovine embryo development in vitro

Gómez

Díez

2000

Animal Reproduction Science

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Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors to support embryo development during the entire culture period. In vitro produced bovine zygotes from slaughterhouse ovaries were cultured in modified-synthetic oviduct Ž . Ž . Ž . Ž . fluid mSOF alone or supplemented with 1 oviductal factors, 2 BSA and 3 FCS. Oviductal factors showed embryotrophic activity, although with blastocyst rates lower than those in BSA and Ž . FCS. Glucose 1.5 mM added at Day 2 of culture did not affect development in the presence of oviductal factors. The number of cells in expanded blastocysts was unaffected by the presence of glucose or any of the protein supplements used. Both BSA and FCS, respectively, improved blastocyst rates of Day 6 embryos produced with oviductal factors. The effect of oviductal factors was masked by the presence of BSA during the entire culture. FCS promoted an earlier appearance of blastocysts. It is concluded that the effect of glucose on in vitro embryo development depends upon the source of protein. Oviductal factors are not an appropriate supplement for embryos beyond Day 6 of culture in SOF, although blastocyst rates of such embryos may be increased by culturing them in the presence of FCS or BSA. q

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“…An obvious deficiency in KSOM is the lack of amino acids as precursors for protein synthesis by the blastocyst. Another deficiency at this stage could be the low glucose concentration, as glucose has been found to be especially important at the time of blastocyst formation [8,10,27,28]. However, glucose is not the only source of energy in KSOM.…”

Section: Discussionmentioning

confidence: 99%