Effect of the preparation conditions on the surface-enhanced Raman-spectrometric identification of thin-layer-chromatographic spots

Critical evaluation of experimental conditions influencing the surface-enhanced Raman spectroscopic (SERS) detection of substances separated by layer liquid chromatographic techniques

“…Because of its simplicity, the application of metal sols has proved most advantageous in the study of real chromatographic systems [ 10]. Because the thin layer itself has a characteristic particlesize distribution, it also participates in the formation of the microsurface.…”

“…Although such work has been confined to model systems only, genuine information has been obtained by assigning bands in the SERS spectra [8,9]. As reported earlier by these authors, the quality of the metal used to provide the enhancement effect, the techniques used for sample preparation, and the analyte-tometal ratio can significantly influence spectral quality [10]. In this study ascorbigen (Asc) and l'-methylascorbigen (MeAsc) have been used to demonstrate that the quality of the chromatographic plate used, the method of its development, and the properties of the analyte (stability, changes of conformation on the stationary phase) also affect spectral quality ( Figure 1 ).…”

SERS and Separation Science

“…Using citrate-reduced silver colloids, which had been pre-aggregated through the addition of sodium perchlorate, the authors were able to record clear micro-FT SERS spectra of the carotenoid, crocetin, at 10 −5 M concentration and calculated that this corresponded to an analysed mass of 0.02 fg [27]. Other experimental factors, including the choice of metal used for the SERS surface, development method and laser excitation wavelength, that affect the success of TLC-SERS measurements have been discussed in more detail by Kocsis et al [28] and Horvarth et al [29].…”

Basic TLC Techniques, Materials, and Apparatus

“…It was found that the type of plate and the development procedure (traditional vs. OPLC) influenced significantly the quality of SERS spectra obtained (213). NIR-SERS was shown to be useful for both qualitative and quantitative analysis in a single step (214).…”