Effect of the oat, horse chestnut, cowherb, soy, quinoa and soapwort extracts on skin‐mimicking monolayers and cell lines

Abstract

The main goal of the study was to compare the effect of aqueous extracts of oat (Avena sativa L.), horse chestnut (Aesculus hippocastanum L.), quinoa (Chenopodium quinoa Willd.), soapwort (Saponaria officinalis L.), cowherb (Vaccaria hispanica [P. Mill.] Rauschert) and soy (Glycine max L.) on model lipid monolayers mimicking the lipid membrane of keratinocytes and intercellular lipids of stratum corneum, and on human skin-related cell lines. Two lipid monolayers, consisting of a dipalmitoylphosphatidylcholine (DPPC) and cholesterol mixture in a molar ratio of 7:3 and Ceramide VI, stearic acid and cholesterol in a molar ratio of 1:1:0.7, and two cell lines (human keratinocyte HaCaT and human skin malignant melanoma A375) were employed. None of the extracts reduced surface pressure below the level achieved for bare monolayers. The strength of the effect on the lipid monolayers (horse chestnut > cowherb > soapwort > soy) points to the existence of some specific interactions responsible for the observed affinity of biosurfactants from the extracts to the lipids in the monolayers. The cytotoxicity tests performed with two model skin cell lines showed that all six plants extracts significantly reduced the cells' viability in a concentration-dependent way. The model lipid monolayers were not solubilized by the investigated surface-active extracts. The latter thus proved interesting candidates for application in mild cleansing cosmetic formulations. Penetration of the monolayers by surface-active components of some extracts, especially horse chestnut, cowherb and soapwort, opens new possibilities for topical delivery of active components.

Antihistamine and Wound Healing Potential of Gold Nanoparticles Synthesized Using Bulbine frutescens (L.) Willd

“… 31 Jurek et al (2021) indicated that preservatives consisting of sodium benzoate and potassium sorbate at a 1:1 ratio induced cytotoxicity on HaCaT cells. 32 This suggests that the significant reduction in wound closure displayed by BFS and BFSAuNPs could be due to the preservatives used during the preparation of the extract.…”

A Sebum-Mimetic Lipid Monolayer and Its Interaction with (Bio)Surfactants

“…In our previous reports we have compared synthetic and natural surfactants extracted from plants in terms of their effect on cell cultures of keratinocytes [24,25]. We have also attempted to correlate the keratinocytes' cytotoxicity with two assays used to quantify the protein (zein test) and lipid (oil emulsification test) solubilization potential [25].…”