Effect of the Nursing Mother on the Gut Microbiome of the Offspring During Early Mouse Development

Treichel, Nicole; Prevoršek, Zala; Mrak, Vesna; Kostrić, Matea; Vestergaard, Gisle; Foesel, Bärbel U.; Pfeiffer, Stefan; Stres, Blaž; Schöler, Anne; Schloter, Michael

doi:10.1007/s00248-019-01317-7

Cited by 16 publications

(13 citation statements)

References 45 publications

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“…An250, Bacteroides nordii, Intestinimonas butyriciproducens) being present in 3 out of 7 datasets (Figure 3A). This observation is particularly important as we could find extensive reports of associating them negatively with BMI and protective roles against metabolic syndrome, implying that they could be markers of a healthy gut (Brahe et al, 2015; Tagliamonte et al, 2021; Treichel et al, 2019). Taken together, our univariate analysis failed to identify any reproducible taxa across the datasets as no species were found to be differentially abundant in at least 4 out of 7 datasets (Figure 3A).…”

Section: Resultsmentioning

confidence: 98%

Meta-analysis reveals obesity associated gut microbial alteration patterns and reproducible contributors of functional shift

Chanda

2022

Preprint

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Cohort-specific 16S rRNA sequence-based studies associating gut microbiota with obesity are often marred with contradictory findings regarding community structure and composition leading to reproducibility crisis of the signals. Moreover, taxonomic drivers of the obesity-linked gut microbial functional imbalances and their replicability also remains unexplored which should be useful for in-depth understanding of obese host-gut microbiota interaction and, strategizing therapeutics. We addressed these questions through unbiased meta-analysis and further machine-learning validation of 692 curated fecal whole metagenomic sequence datasets from diverse geographical locations. Further, obesity-linked pathway shifts were traced back to their specific drivers by integrating the species and pathway profiles through genomic content of the species. We found reproducible depletion of diversity in obese gut microbiome without any pattern in Firmicutes/Bacteroidetes ratio. Additionally, we also identified obesity-linked robust and reproducible gut microbial species and pathway features. Contributors of these pathway features identified as both dataset-specific and shared across the datasets.

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Section: Resultsmentioning

confidence: 98%

Meta-analysis reveals obesity associated gut microbial alteration patterns and reproducible contributors of functional shift

Chanda

2022

Preprint

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“…Given the well-established link between gut microbiota and social behavior (for review see Sherwin et al, 2019), along with the fact that we observed corresponding male-specific changes in the gut microbiome and social behavior, we next asked whether shifting the gut microbiome towards a CON-typical composition would prevent social behavior deficits in DEP/MS male offspring. Cross-fostering on the day of birth has been shown to reliably shift the composition of the offspring gut microbiome to match that of the foster mother over the birth mother (Daft, et al 2015; Treichel et al, 2019; Daoust et al, 2021). DEP/MS exposed pups were fostered to either a different DEP/MS dam on the day of birth (D → fD) or to a CON dam (D→ fC).…”

Section: Resultsmentioning

confidence: 99%

Social deficits induced by pervasive environmental stressors are prevented by microbial or dopaminergic modulation

Smith

Rendina

Kingsbury

et al. 2022

Preprint

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Environmental toxicant exposure, including air pollution, is increasing worldwide. However, toxicant exposures are not equitably distributed. Rather, low-income and minority communities bear the greatest burden, along with higher levels of psychosocial stress. Both air pollution and maternal stress during pregnancy have been linked to neurodevelopmental disorders such as autism, but biological mechanisms and targets for therapeutic intervention remain poorly understood. We demonstrate that combined prenatal exposure to air pollution (diesel exhaust particles, DEP) and maternal stress (MS) in mice induces social behavior deficits only in male offspring, in line with the male bias in autism. These behavioral deficits are accompanied by changes in microglia and dopaminergic circuits in the brain, along with changes in the structure of the gut epithelium and microbiome. Importantly, DEP/MS-induced social deficits in males are prevented by shifting the gut microbiome by cross-fostering at birth and reversed by chemogenetic activation of the dopamine system.

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“…Clostridiales members have been characterized as defenders against obesity in high fat diet induced mice, which is mediated by their metabolites, including butyrate ( Pérez-Matute et al, 2015 ). Anaeromassilibacillus , like Clostridiales , is a SCFA producer and protected mice against obesity ( Treichel et al, 2019 ). Here, the lean-line broilers with significantly enriched C. bacterium and Anaeromassilibacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…An200 , and C. bacterium ) were positively correlated with immune relevant genes. The three species are SCFA producers, which benefit healthier enteric performance ( Qu et al, 2019 ; Treichel et al, 2019 ). Their metabolites, SCFAs, have been proven to maintain a lower pH value and more energy, which could inhibit pathogen colonization ( Venegas et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Multi-Omics Association Reveals the Effects of Intestinal Microbiome–Host Interactions on Fat Deposition in Broilers

et al. 2022

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Growing evidence indicates that gut microbiota factors cannot be viewed as independent in the occurrence of obesity. Because the gut microbiome is highly dimensional and complex, studies on interactions between gut microbiome and host in obesity are still rare. To explore the relationship of gut microbiome–host interactions with obesity, we performed multi-omics associations of gut metagenome, intestinal transcriptome, and host obesity phenotypes in divergently selected obese–lean broiler lines. Metagenomic shotgun sequencing generated a total of 450 gigabases of clean data from 80 intestinal segment contents of 20 broilers (10 of each line). The microbiome comparison showed that microbial diversity and composition in the duodenum, jejunum, ileum, and ceca were altered variously between the lean- and fat-line broilers. We identified two jejunal microbes (Escherichia coli and Candidatus Acetothermia bacterium) and four cecal microbes (Alistipes sp. CHKCI003, Ruminococcaceae bacterium CPB6, Clostridiales bacterium, and Anaeromassilibacillus sp. An200), which were significantly different between the two lines (FDR < 0.05). When comparing functional metagenome, the fat-line broilers had an intensive microbial metabolism in the duodenum and jejunum but degenerative microbial activities in the ileum and ceca. mRNA-sequencing identified a total of 1,667 differentially expressed genes (DEG) in the four intestinal compartments between the two lines (| log2FC| > 1.5 and FDR < 0.05). Multi-omics associations showed that the 14 microbial species with abundances that were significantly related with abdominal fat relevant traits (AFRT) also have significant correlations with 155 AFRT-correlated DEG (p < 0.05). These DEG were mainly involved in lipid metabolism, immune system, transport and catabolism, and cell growth-related pathways. The present study constructed a gut microbial gene catalog of the obese–lean broiler lines. Intestinal transcriptome and metagenome comparison between the two lines identified candidate DEG and differential microbes for obesity, respectively. Multi-omics associations suggest that abdominal fat deposition may be influenced by the interactions of specific gut microbiota abundance and the expression of host genes in the intestinal compartments in which the microbes reside. Our study explored the interactions between gut microbiome and host intestinal gene expression in lean and obese broilers, which may expand knowledge on the relationships between obesity and gut microbiome.

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Effect of the Nursing Mother on the Gut Microbiome of the Offspring During Early Mouse Development

Cited by 16 publications

References 45 publications

Meta-analysis reveals obesity associated gut microbial alteration patterns and reproducible contributors of functional shift

Meta-analysis reveals obesity associated gut microbial alteration patterns and reproducible contributors of functional shift

Social deficits induced by pervasive environmental stressors are prevented by microbial or dopaminergic modulation

Multi-Omics Association Reveals the Effects of Intestinal Microbiome–Host Interactions on Fat Deposition in Broilers

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