Effect of the NO donor “sodium nitroprusside” (SNP), the ethylene inhibitor “cobalt chloride” (CoCl2) and the antioxidant vitamin E “α-tocopherol” on in vitro shoot proliferation of Sideritis raeseri Boiss. &amp; Heldr. subsp. raeseri

Sarropoulou, Virginia; Maloupa, Eleni

doi:10.1007/s11240-016-1139-6

Cited by 26 publications

(10 citation statements)

References 44 publications

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“…[14][15][16][17][18] The findings of this present study also support the previous studies, revealing that biotic and abiotic elicitors increase the accumulation of caffeic acid and alkamide. In previous studies carried out in Echinacea purpurea species, root hair cultures were used and higher caffeic acid and alkamide accumulation were obtained, compared to our study.…”

Section: Resultssupporting

confidence: 89%

Enhancement of Phytochemical Compounds Using Biotic and Abiotic Elicitors in Purple Coneflower (Echinacea purpurea L.)

Açıkgöz¹,

Yarilgaç²,

Kara³

2018

IJPER

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Background: Phytochemicals also known secondary metabolites, naturally occurring in medicinal and aromatic plants, are of considerable importance for plant survival and human health. Objective: The objective of this study was to increase accumulation of caffeic acid and alkamide, using biotic and abiotic stresses conditions driving cell defense systems, in cell suspension cultures in purple coneflower (Echinacea purpurea L.). Methods: As biotic and abiotic elicitors, yeast extract (0, 25, 50 and 100 mg l

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Section: Resultssupporting

confidence: 89%

Enhancement of Phytochemical Compounds Using Biotic and Abiotic Elicitors in Purple Coneflower (Echinacea purpurea L.)

Açıkgöz¹,

Yarilgaç²,

Kara³

2018

IJPER

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“…The exterior usage of nitric oxide might improve the tolerance of plants under various stresses such as temperature, heavy metals, ultraviolet radiation, drought, and salinity [ 78 – 80 ]. The activation rate of nitric oxide has been evaluated by the exterior usage of sodium nitroprusside (SNP) instead of using NO gas directly because of some technical difficulties [ 81 ]. In recent years, nitric oxide gets involved in developing in vitro plant propagation [ 82 ].…”

Section: Discussionmentioning

confidence: 99%

Development of support vector machine-based model and comparative analysis with artificial neural network for modeling the plant tissue culture procedures: effect of plant growth regulators on somatic embryogenesis of chrysanthemum, as a case study

et al. 2020

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Background: Optimizing the somatic embryogenesis protocol can be considered as the first and foremost step in successful gene transformation studies. However, it is usually difficult to achieve an optimized embryogenesis protocol due to the cost and time-consuming as well as the complexity of this process. Therefore, it is necessary to use a novel computational approach, such as machine learning algorithms for this aim. In the present study, two machine learning algorithms, including Multilayer Perceptron (MLP) as an artificial neural network (ANN) and support vector regression (SVR), were employed to model somatic embryogenesis of chrysanthemum, as a case study, and compare their prediction accuracy. Results: The results showed that SVR (R 2 > 0.92) had better performance accuracy than MLP (R 2 > 0.82). Moreover, the Non-dominated Sorting Genetic Algorithm-II (NSGA-II) was also applied for the optimization of the somatic embryogenesis and the results showed that the highest embryogenesis rate (99.09%) and the maximum number of somatic embryos per explant (56.24) can be obtained from a medium containing 9.10 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 4.70 μM kinetin (KIN), and 18.73 μM sodium nitroprusside (SNP). According to our results, SVR-NSGA-II was able to optimize the chrysanthemum's somatic embryogenesis accurately. Conclusions: SVR-NSGA-II can be employed as a reliable and applicable computational methodology in future plant tissue culture studies.

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“…Successful bud initiation (100%) was achieved on semisolid medium containing MS basal salt mixture; MSbased media have been shown to be suitable for various other herbaceous plant species (Sarropoulou and Maloupa 2017;Spinoso-Castillo et al 2017). Cytokinins can affect plant growth in a variety of ways, but are recognized as a requirement for cell division and differentiation, while also being widely used to promote shoot multiplication.…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation and reintroduction of golden paintbrush (Castilleja levisecta), a critically imperilled plant species

et al. 2018

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Golden paintbrush (Castilleja levisecta Greenm.) is a hemiparasitic herbaceous perennial native to the Pacific Northwest of North America and is considered critically imperilled with only 11 populations remaining in the wild. The main objective of this study was to develop ex situ and in situ conservation through micropropagation and field plantings. In vitro cultures were initiated using nodal explants from two plants raised from seeds collected from a natural population. Shoots were then multiplied on Murashige and Skoog basal medium with 2.0 μmol L−1 6-benzylaminopurine (BA), 3.0 μmol L−1 kinetin (Kn), 2.2 g L−1 phytagel, and 3% sucrose. Explant position on source plants, culture vessel design, and application of different plant growth regulator levels for BA, Kn, and thiadiazuron (TDZ) were tested to optimize micropropagation protocols. Clones from the plants showed differences in plant height and number of nodes in response to various BA and TDZ concentrations. In vitro shoots were successfully rooted under ex vitro conditions using commercial rooting powder (0.8% indole-3-butyric acid) with an average of ∼17 roots per shoot and acclimatized in the greenhouse with 100% survival rate. Two-month-old plants were transferred to a Parks Canada restoration site at Fort Rodd Hill, Victoria, BC, with 7.5% survival. The use of micropropagation in combination with reintroduction efforts offers an excellent opportunity for conserving endangered plant biodiversity in vitro and facilitating in situ conservation efforts by providing plants for reintroduction.

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Effect of the NO donor “sodium nitroprusside” (SNP), the ethylene inhibitor “cobalt chloride” (CoCl2) and the antioxidant vitamin E “α-tocopherol” on in vitro shoot proliferation of Sideritis raeseri Boiss. & Heldr. subsp. raeseri

Cited by 26 publications

References 44 publications

Enhancement of Phytochemical Compounds Using Biotic and Abiotic Elicitors in Purple Coneflower (Echinacea purpurea L.)

Enhancement of Phytochemical Compounds Using Biotic and Abiotic Elicitors in Purple Coneflower (Echinacea purpurea L.)

Development of support vector machine-based model and comparative analysis with artificial neural network for modeling the plant tissue culture procedures: effect of plant growth regulators on somatic embryogenesis of chrysanthemum, as a case study

In vitro propagation and reintroduction of golden paintbrush (Castilleja levisecta), a critically imperilled plant species

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