Effect of the nitrogen status on copper accumulation and pools of metal-binding peptides in the planktonic diatom Thalassiosira pseudonana1Communication no. 2404 of NIOO, Netherlands Institute of Ecology.1

Rijstenbil, J.W.; Dehairs, F.; Ehrlich, Robert S.; Wijnholds, Jan

doi:10.1016/s0166-445x(97)00091-x

Cited by 66 publications

(48 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although nutrient concentrations were not measured, it is possible that nutrient depletion caused the observed decreases in particulate thiol concentrations with the shift from exponential to stationary growth. Cultures of Thalassiosira pseudonana grown under nitrogen-limiting conditions have lower ambient levels of glutathione and phytochelatins (Rijstenbil et al 1998), and nutrient limitation has been suggested to constrain the synthesis of phytochelatin in response to metal stress in some marine algae (Ahner et al 2002). Sulfate assimilation can be curtailed by nitrogen limitation in higher plants (Koprivova et al 2000), which might explain the effects observed here.…”

Section: Discussionmentioning

confidence: 69%

Effects of copper, cadmium, and zinc on the production and exudation of thiols by Emiliania huxleyi

Dupont

Ahner

2005

Limnol. Oceanogr.

View full text Add to dashboard Cite

Cultures of the ubiquitous coccolithophore Emiliania huxleyi grown at field-relevant fixed free ion concentrations of Cu, Cd, and Zn exude a broad array of thiols, some of which increase with increasing metal ion concentration. The primary thiols released are contingent on the particular metal or combination of metals added to the culture media. Exposure to Cu results in the release of arginine-cysteine, glutamine-cysteine, and cysteine; Cd causes these thiols and glutathione to be released; and high Zn results in the synthesis and exudation of predominantly ␥-glutamate-cysteine (␥-Glu-Cys). Because the free ion concentrations of Cu and Cd used in these experiments are similar to those observed in the field, active exudation could be an important source of thiols to surface seawater and thus might affect trace metal speciation in the open ocean. Intracellular thiol concentrations were also clearly affected by metal concentrations, with ␥-Glu-Cys being particularly dynamic. Additionally, the shift from exponential to stationary growth in batch cultures caused approximately two-to fourfold declines in the concentrations of nearly all intracellular thiols.There is evidence that sulfur-containing compounds of low molecular mass are an important component of the pool of ligands responsible for the nearly complete complexation of (Moffett et al. 1990;Bruland et al. 1991), and culture studies have confirmed that phytoplankton have the capability of producing ligands with similar complexing strengths to those measured in the field (Moffett and Brand 1996; Leal et al. 1999;Croot et al. 2000). Specifically, Leal et al. (1999) found that high copper concentrations promoted the simultaneous release of copper-binding ligands and compounds with reduction potentials similar to those of known thiols.We identified two novel thiols produced by Emiliania huxleyi, arginine-cysteine (Arg-Cys) and glutamine-cysteine (Gln-Cys), which were present at high intracellular concentrations (Dupont et al. 2004b). These compounds, along with cysteine, were released proportionally as copper was added to the growth media. Specific thiol-Cu(I) complexes-CysCu(I), Arg-Cys-Cu(I)-Cys, and (Cys) 2 -Cu(I)-were also identified in the culture media of copper-exposed E. huxleyi with matrix-assisted laser desorption/ionization time-offlight mass spectrometry. Given that the culture techniques used in that study were designed to produce high concentrations of copper ligand complexes, it remains to be determined whether the exudation of these thiols occurs at en-1 To whom correspondence should be addressed. Present address: Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093 (cdupont@ucsd.edu). AcknowledgmentsThis work was funded by an NSF grant (OCE-0002677) to B.A.A. We thank T. Goepfert for help in algal culturing and sampling and two anonymous reviewers for helpful comments and suggestions.

show abstract

Section: Discussionmentioning

confidence: 69%

Effects of copper, cadmium, and zinc on the production and exudation of thiols by Emiliania huxleyi

Dupont

Ahner

2005

Limnol. Oceanogr.

View full text Add to dashboard Cite

show abstract

“…Albergoni et al (1980) and Rijstenbil et al (1998) suggested that some of the algae capable to produce metal binding compounds there from get the ability to bind and sequester copper ions in the cytoplasm and reduce toxicity. On the other hand, the toxic effect of copper at higher concentrations (10 −7 and 10 −6 M) detected in the present study may be due to the oxidative potential of copper (II) that causes reduction of chlorophyll and decrease of oxygen evolution rates and depletion of ATP by inhibition of enzymes (nitrate reductase and alkaline phosphatase) which are involved in nitrate and ammonia cellular metabolism (Muwafq and Bernd, 2006).…”

Section: Resultsmentioning

confidence: 99%

Toxicological Response of the Green Alga <i>Chlorella vulgaris</i>, to Some Heavy Metals

Afkar¹

2010

American Journal of Environmental Sciences

View full text Add to dashboard Cite

Problems statement:The disturbance of aquatic ecosystems provoked by heavy metals pollution from industrial and domestic sources, has as consequence the loss of biological diversity, as well as increased bioaccumulation and magnification of toxicants in the food chain. The aim of this study was to evaluate the effect of some heavy metals on some physiological activities of Chlorella vulgaris beyerinck with special references to metal bioaccumulation. Approach: Chlorella vulgaris Beyerinck was isolated from Al-Asfar Lake, Al-Hassa, Saudi Arabia. A standard initial inoculum of the isolated algae was inoculated to culture flasks. The culture flasks were supplied with various concentrations of Cobalt, Copper and Zinc ranging from 10 −6 -10 −9 M. At the end of the incubation period cultures were filtered and washed several times by distilled water for measurements the various experimental parameters. Results: The data show that the lower doses of the three tested metals had stimulatory effect in biomass yield of Chlorella vulgaris, whereas the higher doses were inhibitory depending on the type of the metal. The inhibitory effect of copper to the growth parameters of Chlorella vulgaris was more pronounced than other two tested metals. The total protein content, total carbohydrate and the total free amino acids of the tested green alga Chlorella vulgaris gradually decreased in a manner dependent on the metal concentration in the medium. On the other hand, bioaccumulation of cobalt, copper and zinc by Chlorella vulgaris cells were parallel to increasing the concentrations in the culture medium. Conclusion: The inhibitory and stimulatory effects of either of the used heavy metals depend on concentration. Different organisms, however, have different sensitivities to the same metal and the same organisms may be more or less damaged by different metals. The uptake of an element from the surrounding medium is seldom exactly proportional to the amount present in the water.

show abstract

“…In marine algae, recent studies demonstrated that N enrichments substantially enhance the accumulation of Cd and Zn by marine diatoms (Wang et al 2001a,b). Rijstenbil et al (1998) also showed that N-addition significantly increased cellular Cu, Mn, and Zn accumulation in the diatom Thalassiosira pseudonana during a 14 d batch culture. Increasing glutathione synthesis was proposed to 171 Aquat Microb Ecol 35: 163-173, 2004 account for the enhanced Zn uptake by the diatoms.…”

mentioning

confidence: 79%

Biological uptake of Cd, Se(IV) and Zn by Chlamydomonas reinhardtii in response to different phosphate and nitrate additions

Wang

2004

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

We investigated the influences of different nutrient regimes on the accumulation of Cd, Zn, and Se(IV) in the freshwater green alga Chlamydomonas reinhardtii over a 4 h exposure period. After the cells had been acclimated to different ambient P levels from 0.1 to 10.0 µM for 2 d in different media (buffer, basic and simplified uptake media), their intracellular metal concentrations increased by 3.6 to 14× for Cd and 1.6 to 4.0× for Zn. Se uptake was, however, decreased by 7.7 to 44×. Semi-continuous culture experiments further demonstrated that the alga's metal concentrations were enhanced 26× for Cd and 8.0× for Zn, whereas Se accumulation was inhibited 75× at the same medium P levels. The uptake rates in semi-continuous cultures increased by 269× for Cd and 11× for Zn, but Se uptake decreased 92× with increasing P concentration. Medium P additions also significantly increased intracellular partitioning for Cd and Zn, but there was no apparent effect for Se. For P-starved or P-repleted cells, the P-enriched medium dramatically increased the cellular accumulation of Cd and Zn compared with the P-depleted medium. A surge uptake of Cd and Zn occurred in P-starved cells. P starvation also resulted in a remarkable increase in Se accumulation in the Pdeplete medium. N enrichment from 5 to 200 µM significantly increased the alga's uptake and intracellular partitioning for Cd and Zn, although the effects were not strictly concentration-dependent. Our study strongly suggests that ambient P enrichment greatly stimulates Cd and Zn accumulation and sequestration in C. reinhardtii, presumably due to rapid P uptake and subsequent formation of polyphosphate in the cells, and distinctly inhibits Se uptake possibly due to the competition of similar anionic forms for transport between Se and P. KEY WORDS: Uptake · Metals · Green alga · Nutrient enrichment Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 35: [163][164][165][166][167][168][169][170][171][172][173] 2004 concentrations in phytoplankton, especially in freshwater species, although this may be important in understanding the stoichiometric mechanisms in lake systems.Under N-limited conditions, a significant reduction in photosynthetic activity occurs in green algae, including a sharp decline in their chlorophyll content and a rise in carotenoid concentration (Sayed 1998, Hanagata & Dubinsky 1999. When the phytoplankton are under P-limited conditions, the uptake system for phosphate in cells is highly activated. Abrupt exogenous P enrichment results in a rapid P incorporation and storage in intracellular polyphosphates (Kulaev & Vagabov 1983, Wagner & Falkner 2001. Polyphosphate bodies are considered an important pool for the sequestration of heavy metals by freshwater phytoplankton (e.g. Cd, Hg, Pb, and Zn) (Jensen et al. 1982a). Although metal limitation (e.g. of Fe) in phytoplankton may affect the assimilation of nitrate and phosphate (McKay et al. 2001), the effects of nutrient enrichment or starvation on m...

show abstract

Effect of the nitrogen status on copper accumulation and pools of metal-binding peptides in the planktonic diatom Thalassiosira pseudonana1Communication no. 2404 of NIOO, Netherlands Institute of Ecology.1

Cited by 66 publications

References 31 publications

Effects of copper, cadmium, and zinc on the production and exudation of thiols by Emiliania huxleyi

Effects of copper, cadmium, and zinc on the production and exudation of thiols by Emiliania huxleyi

Toxicological Response of the Green Alga <i>Chlorella vulgaris</i>, to Some Heavy Metals

Biological uptake of Cd, Se(IV) and Zn by Chlamydomonas reinhardtii in response to different phosphate and nitrate additions

Contact Info

Product

Resources

About