Effect of the nitrogen source on the hydrogen production metabolism and hydrogenases of Clostridium butyricum CWBI1009

Hamilton, Christopher; Całusińska, Magdalena; Baptiste, Stacy; Masset, Julien; Beckers, Laurent; Thonart, Philippe; Hiligsmann, Serge

doi:10.1016/j.ijhydene.2017.12.162

Cited by 31 publications

(3 citation statements)

References 37 publications

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“…The literature also shows that hydrogenase is present in bacteria such as Enterobacter , Clostridium , and Klebsiella . These microorganisms are also potential contributors to the production of hydrogen [ 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam

Zhao

Xia

2018

Biotechnol Biofuels

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BackgroundBiogenic and biogenic-thermogenic coalbed methane (CBM) are important energy reserves for unconventional natural gas. Thus, to investigate biogenic gas formation mechanisms, a series of fresh coal samples from several representative areas of China were analyzed to detect hydrogen-producing bacteria and methanogens in an in situ coal seam. Complete microbial DNA sequences were extracted from enrichment cultures grown on coal using the Miseq high-throughput sequencing technique to study the diversity of microbial communities. The species present and differences between the dominant hydrogen-producing bacteria and methanogens in the coal seam are then considered based on environmental factors.ResultsSequences in the Archaea domain were classified into four phyla and included members from Euryarchaeota, Thaumarchaeota, Woesearchaeota, and Pacearchaeota. The Bacteria domain included members of the phyla: Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria, Verrucomicrobia, Planctomycetes, Chloroflexi, and Nitrospirae. The hydrogen-producing bacteria was dominated by the genera: Clostridium, Enterobacter, Klebsiella, Citrobacter, and Bacillus; the methanogens included the genera: Methanorix, Methanosarcina, Methanoculleus, Methanobrevibacter, Methanobacterium, Methanofollis, and Methanomassiliicoccus.ConclusionTraces of hydrogen-producing bacteria and methanogens were detected in both biogenic and non-biogenic CBM areas. The diversity and abundance of bacteria in the biogenic CBM areas are relatively higher than in the areas without biogenic CBM. The community structure and distribution characteristics depend on coal rank, trace metal elements, temperature, depth and groundwater dynamic conditions. Biogenic gas was mainly composed of hydrogen and methane, the difference and diversity were caused by microbe-specific fermentation of substrates; as well as by the environmental conditions. This discovery is a significant contribution to extreme microbiology, and thus lays the foundation for research on biogenic CBM.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1237-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam

Zhao

Xia

2018

Biotechnol Biofuels

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“…In addition, the proposed process also integrates the photocatalysis and micro-electrolysis processes for the sake of maximizing hydrogen productions of the entire process. The challenges of this proposed integrated process lies in: (a) the pH swing between the steps of the photofermentation stage, where ammonia will be generated continuously during the photofermentation catalyzed by nitrogenase, and the nearly neutral pH value of DF; (b) how to best optimize the feeding concentration of organic substrates (C/N/O ratio) [144] and control different metabolic pathways on the level of genetic expressions, as this will significantly affect the level of genetic diversity expressions during the fermentations [145][146][147]; (c) eco-friendly access to the water available.…”

Section: Future Perspectivesmentioning

confidence: 99%

A Review of the Enhancement of Bio-Hydrogen Generation by Chemicals Addition

Sun

Yang

et al. 2019

Catalysts

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Bio-hydrogen production (BHP) produced from renewable bio-resources is an attractive route for green energy production, due to its compelling advantages of relative high efficiency, cost-effectiveness, and lower ecological impact. This study reviewed different BHP pathways, and the most important enzymes involved in these pathways, to identify technological gaps and effective approaches for process intensification in industrial applications. Among the various approaches reviewed in this study, a particular focus was set on the latest methods of chemicals/metal addition for improving hydrogen generation during dark fermentation (DF) processes; the up-to-date findings of different chemicals/metal addition methods have been quantitatively evaluated and thoroughly compared in this paper. A new efficiency evaluation criterion is also proposed, allowing different BHP processes to be compared with greater simplicity and validity.

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“…O nitrogênio é removido pela transformação em nitrogênio gasoso ou íon amônio. Como íon amônio ocorre à assimilação biólogica, por esta via conhece-se que o gênero Clostridium pode assimilar nitrogênio na forma orgânica e como íon amônio Hamilton et al, 2018).…”

Section: Caracterização Da Mfc Por Espectroscopia De Impedância Eletroquímica (Eis)unclassified

Célula a Combustível do tipo Microbiana alimentada com efluente de laticínios: Estudo da recuperação de energia e do potencial remediador em sistemas de compartimento único

Marassi¹

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Microbial Fuel Cells (MFC) are bioelectrochemical devices capable of producing direct electrical energy from electrogenic microorganisms as catalysts toward a substrate oxidation reaction. Currently, the MFC research is focused in substrates that are normally passive environmental as wastewater, which are treated and simultaneously with power generation. In this context, the dairy wastewater has been studied for MFC substrates because this wastewater has a favorable composition, as amount of biodegradable organic matter, high ionic conductivity and significant nutrient concentrations. In order to diffuse MFC as environmental technology for dairy wastewater treatment, there are a lack of technological knowledge toward to improve energy production performance and studies that address the issues of inorganic pollutants and toxicity removal. For this reason, the present thesis was developed with the construction of four different architectures of MFCs to produce direct electrical energy with the removal of pollutants from the dairy effluent. The air-cathode MFCs were constructed from acrylic as tubular with internal circulation (TCI), tubular biofilter (TB), tubular hybrid upflow (THA) and scaled cubic upflow (CA) bioreactor. The systems were lead in semi-continuous feeding mode with long-term operation using the startup consortium of Shewanella oneidenisis and Clostridium butyricum electrogenic bacteria. The MFCs produced the maximum potential of 649 mV, 540 mV, 616 mV and 583 mV, which resulted in high maximum power densities of 1.3 W/m 3 , 1.1 W/m 3 , 0.5 W/m 3 and 3.5 W/m 3 for TCI, TB, CA and THA, respectively. Notably, the hybrid tubular showed the best performance in the electricity generation. In terms of treatment, MFCs showed high COD and BOD removal with values above 90%, but only the MFC-TCI had a low COD and BOD perfomance with 62% for COD and 46% for BOD. In terms of, the nitrogen and phosphorus removal, the MFC-TB had the best performance with 85% and 92%, respectively, but the MFC-THA also presented significant values for nitrogen and phosphorus as 43% and 95%, respectively. Acute toxicity studies were applied for MFCs TB, CA and THA, in which non acute toxic effect was exhibited in dairy wastewater treated. The chronic toxicity assays were tested only to MFC-THA, which revealed chronic effect for treated effluent concentration in 40%. MFCs also showed high efficiency for nitrate and sulfate removal. Hybrid upflow MFC demonstrated exceptional results that should be explored at larger scalar, in this case electrochemical impedance spectroscopy also showed changes in the anode caused by the exchange of external resistance that is associated with high performance.

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Effect of the nitrogen source on the hydrogen production metabolism and hydrogenases of Clostridium butyricum CWBI1009

Cited by 31 publications

References 37 publications

The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam

The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam

A Review of the Enhancement of Bio-Hydrogen Generation by Chemicals Addition

Célula a Combustível do tipo Microbiana alimentada com efluente de laticínios: Estudo da recuperação de energia e do potencial remediador em sistemas de compartimento único

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