Effect of the methanolic extract of Brachylaena discolor in a streptozotocin-induced diabetic rat model

Mellem, John J.

doi:10.5897/ajpp12.979

Cited by 6 publications

(6 citation statements)

References 19 publications

(18 reference statements)

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“…This is the first study to examine the effect of aqueous extract of B. elliptica in INS-1 cell death and proliferation. However, aqueous and methanol extracts of Brachylaena discolor have been reported to possess a proliferative effect on Hela cell lines [ 29 ] which supported the findings obtained in this study. This result gives an indication that the aqueous leaf extract of B. elliptica has a proliferative or anti-apoptotic effect or both and could benefit patients suffering from diabetes.…”

Section: Discussionsupporting

confidence: 91%

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

Sagbo

Venter

Koekemoer

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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In South Africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies are frequently reported to have adverse side effects. Ethnomedicinal plant use has shown promise for the development of cheaper, cost-effective antidiabetic agents with fewer side effects. The aim of this study was to investigate the antidiabetic activity and mechanism of action of aqueous leaf extract prepared from Brachylaena elliptica. The potential of the extract for cytotoxicity was evaluated using MTT assay in HepG2 cells. The effects of the plant extract on glucose utilization in HepG2 cells and L6 myotubes, triglyceride accumulation in 3T3-L1, INS-1 proliferation, glucose metabolism in INS-1 cells, and NO production in RAW macrophages were also investigated using cell culture procedures. The inhibitory effects of the extract on the activities of different enzymes including alpha-amylase, alpha-glucosidase, pancreatic lipase, dipeptidyl peptidase IV (DPP-IV), collagenase, and CYP3A4 enzymes were evaluated. The extract also tested against protein glycation using standard published procedure. The plant extract displayed low level of toxicity, where both concentrations tested did not induce 50% cell death. The extract caused a significant increase in glucose uptake in HepG2 liver cells, with efficacy significantly higher than the positive control, berberine. The crude extract also displayed no significant effect on muscle glucose uptake, triglyceride accumulation in 3T3-L1, glucose metabolism in INS-1 cells, alpha-amylase, alpha-glucosidase, DPP-IV, lipase, protein glycation, and collagenase compared to the respective positive controls. The extract displayed a proliferative effect on INS-1 cells at 25 μg/ml when compared to the negative control. The plant also produced a concentration-dependent reduction in NO production in RAW macrophages and also demonstrated weak significant inhibition on CYP3A4 activity. The findings provide evidence that B. elliptica possess antidiabetic activity and appear to exact its hypoglycemic effect independent of insulin.

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Section: Discussionsupporting

confidence: 91%

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

Sagbo

Venter

Koekemoer

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Bip . The major mode of action of plants in this family has been reported to be through insulin release, repair of pancreatic β-cells, inhibition of carbohydrate digesting enzymes and oxidative stress [ 23 , 24 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ]. Some of the anti-diabetic molecules isolated are saponins, flavanones, tannins and flavonoids (aglycones) [ 23 , 56 , 64 , 65 , 66 ].…”

Section: Resultsmentioning

confidence: 99%

Medicinal Plants Used for the Traditional Management of Diabetes in the Eastern Cape, South Africa: Pharmacology and Toxicology

Odeyemi

Bradley

2018

Molecules

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The use of medicinal plants for the management of diabetes mellitus is on the rise in the developing countries, including South Africa. There is increasing scientific evidence that supports the claims by the traditional healers. In this review, we compare the families of previously reported anti-diabetic plants in the Eastern Cape by rating the anti-diabetic activity, mode of action and also highlight their therapeutic potentials based on the available evidence on their pharmacology and toxicity. Forty-five plants mentioned in ethnobotanical surveys were subjected to a comprehensive literature search in the available electronic databases such as PubMed, ScienceDirect, Google Scholar and Elsevier, by using “plant name” and “family” as the keywords for the primary searches to determine the plants that have been scientifically investigated for anti-diabetic activity. The search returned 25 families with Asteraceae highly reported, followed by Asphodelaceae and Alliaceae. Most of the plants have been studied for their anti-diabetic potentials in vivo and/or in vitro, with most of the plants having a higher percentage of insulin release and inhibition against carbohydrate digesting enzymes as compared with insulin mimetic and peripheral glucose uptake. Almost all the investigated plants also inhibit oxidative stress as part of their hypoglycemic activity with less toxicity. However, the isolation of their bioactive molecules is still lacking. This review provides a resource to enable thorough assessments of the therapeutic profiles of available medicinal plants used for the management of diabetes in the Eastern Cape, South Africa. Further studies such as the identification of the active ingredients of potent plants still need to be carried out; this may lead to new molecules in drug discovery and development.

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“…Leaf infusion or decoction taken orally (Mellem, 2013), (Mellem et al, 2015) Respiratory infections (chest pains, cough, sore throat and tuberculosis)…”

Section: Renal Problemsmentioning

confidence: 99%

“…Anti-hyperglycaemic activities (Mellem, 2013) evaluated the anti-hyperglycaemic activities of the methanol extract of B. discolor leaves using a streptozotocin-induced diabetic rat model. The doses of 50.0 mg/ml and 150.0 mg/ml) were administered daily to both streptozotocininduced and control rats and the biochemical pro ile of the rats assessed over 28 days.…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

Review of phytochemistry, biological activities and therapeutic potential of Brachylaena discolor

Maroyi

2020

ijrps

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Brachylaena discolor DC. is a shrub or tree widely used as herbal medicine in southern Africa. Brachylaena discolor is indigenous to Botswana, Eswatini, Mozambique, South Africa, Zambia and Zimbabwe. This study was aimed at reviewing the phytochemistry, biological activities and therapeutic potential of B. discolor. Information on phytochemistry, biological activities and therapeutic potential of B. discolor was collected from online sources such as Google Scholar, PubMed and Science Direct, and pre-electronic sources such as books, book chapters, theses and journal articles obtained from the University library. This investigation revealed that the bark, leaf, root, stem and twig infusion or decoction of B. discolor are mainly used for magical purposes and as anthelmintic and tonic, and traditional medicine for female infertility, skin infections, renal problems, diabetes, gastro-intestinal problems and respiratory infections. Chemical compounds identified from B. discolor include alkaloids, flavonoids, phenolics, phlobatannins, saponins, sesquiterpene lactones, steroids, tannins and terpenoids. Ethnopharmacological review showed that B. discolor and phytochemical compounds identified from the species have anticancer, anthelmintic, anti-hyperglycaemic, antibacterial, cytotoxicity, antifungal, antidiabetic, antioxidant and leishmanicidal activities. Advanced ethnopharmacological research on B. discolor should focus on the possible biochemical mechanisms of both the crude extracts and identified phytochemical compounds including toxicological, in vivo and clinical studies to corroborate the traditional medicinal applications of the species.

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Effect of the methanolic extract of Brachylaena discolor in a streptozotocin-induced diabetic rat model

Cited by 6 publications

References 19 publications

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

Medicinal Plants Used for the Traditional Management of Diabetes in the Eastern Cape, South Africa: Pharmacology and Toxicology

Review of phytochemistry, biological activities and therapeutic potential of Brachylaena discolor

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