Effect of the leaf extract from cauliflower (brassica oleracea L. var. botrytis) on the biodistribution of the radiopharmaceutical sodium pertechnetate in mice and on the electrophoretic mobility of plasmid pUC 9.1 DNA

Abstract

There is evidence that the biodistribution of radiopharmaceuticals may be altered by a variety of drugs. If unknown, the drug interaction with radiopharmaceuticals can lead to misdiagnosis or the necessity to repeat the examination, increasing the dose to the patient. Different substances has the ability to modify the electrophoretic mobility of the deovibonucleic acid (DNA). Cauliflower has the leaves used in folk medicine. We evaluated the influence of the leaf extract of cauliflower on: (i) the biodistribution of the radiopharmaceutical sodium pertechnetate (99"Tc04Na) and in (ii) the mobility of a plasmid pUC 9.1 through gel electrophoresis analysis. 99"TcOjNa (0.3ml) was administered into female Wistur rats which had drunk or not the extract for 60 days. AAer 10 min, the animals were sacrificed, the organs isolated, the radioactivity determined in a well counter and the percentages of radioactivity per gam (%ATl/g) in the organs calculated. The %ATI/g was not significantly altered (p>0.05) in the treated animals. The supercoiled form of the plasmid was modified by the cauliflower extract. We suggest that the action of this extract on the DNA might be explained by a direct effect or by the generation of reactive oxygen species (ROS) that could be inducing lesions in the plasmid DNA. However, this Iesive effect was not capable to alter the biodistribution of the 99"Tc04Na in the treated animals with the extract. ,IntroductionRadiopharmaceuticals are designed to have specific biodistribution and uptake in determined targets and/or elimination patterns when administered to normal subjects. The altered biologic behavior helps a physician to make a diagnosis (1). Altered biologic behavior may also be due to interferences caused by pharmacodynamic effects of drugs (2). Various substances, as the stannous ions, have the property to induce alterations on the deoxyribonucleic acid (DNA) molecule (3). Cauliflower in fok medicine, has its leaves used to treat a variety of diseases (4). The purpose of this study was to evaluate the effect of the leaf extract of the cauliflower (i) on the biodistribution of the radiopharmaceutical 99"Tc04Na in female FVistar rats and (ii) on the topology on gel electrophoresis of plasmid DNA submitted to SnClt (5). Results and discussionThe effect of the leaf extract of cauliflower in the uptake (%AWg) of 99"Tc04Na in the isolated organs from female Wistar rats which had drunk (treated group) or not

An experimental model to study the effects of a senna extract on the blood constituent labeling and biodistribution of a radiopharmaceutical in rats

“…However, cauliflower extract does not alter the biodistribution of Na 99m TcO 4 in mice. 28 In the present study ( Table II ), the aqueous senna extract decreased the uptake of the Na 99m TcO 4 radiopharmaceutical in the thyroid, liver, pancreas, lungs and blood. The action of the senna extract could generate metabolites capable of promoting morphological and physiological modifications in these organs and altering the biodistribution of Na 99m TcO 4 in the treated animals.…”

“…A biodistribution study has demonstrated an increase in the uptake of Na 99m TcO 4 in the liver30 due to eggplant extract and an altered uptake in the duodenum, spleen, pancreas, stomach and blood16 due to Passiflora edulis flavicarpa extract. However, cauliflower extract does not alter the biodistribution of Na 99m TcO 4 in mice 28. In the present study (Table II), the aqueous senna extract decreased the uptake of the Na 99m TcO 4 radiopharmaceutical in the thyroid, liver, pancreas, lungs and blood.…”

“… 20 , 23 Through in vitro studies, researchers have already verified that extracts of Ginkgo biloba , 26 Mentha crispa , 27 Fucus vesiculosus 21 , cinnamon 20 and Cordia salicifolia 13 decrease the radiolabeling of blood constituents. However, another study 28 of treatment with cauliflower extract showed no alterations in the uptake of 99m Tc by the blood constituents. In in vivo studies of blood samples from animals treated with Ginkgo biloba extract, the effect of this natural product on the labeling of blood constituents with 99m Tc was almost completely eliminated.…”