Effect of the irradiation on Neuroblastoma-derived microvesicles: A physical and biological investigation

Cerreto, M; Sennato, Simona; Tortolici, Flavia; Casciardi, Stefano; Giovanetti, Anna; Rufini, Stefano

doi:10.1016/j.colsurfa.2017.05.029

Cited by 7 publications

(7 citation statements)

References 35 publications

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“…The EVs diameter, measured by CytoFLEX, did not show any significant difference in size distribution between the different dose groups, in agreement with our previous observations using DSL [ 28 ]. More than 80% of EVs were in the range of 40–160 nm, which, according to the ISEV guidelines, represents the size of the exosomes [ 29 ].…”

Section: Discussionsupporting

confidence: 91%

“…Interestingly, CD81 and CD9, whose expression increases in EVs10 compared to EVs0, were both related to the modulation of cell migration linked to PI3K activation [ 26 , 27 ]. The differences in the membrane protein composition that we observed, may reflect the previously described changes in the potential surface of EVs released by SH-SY5Y cells after irradiation [ 28 ].…”

Section: Discussionsupporting

confidence: 65%

“…Abramowicz et al reviewed the role of EVs released by cells subjected to environmental physicochemical stress in regulating proliferation, cell survival, migration, and angiogenesis [ 15 ]. However, to our knowledge, this work together with Cerreto et al [ 28 ] are the first studies proving that EVs released shortly after X-rays radiation can induce the activation of survival pathways that correlate with EMT in recipient cells.…”

Section: Discussionmentioning

confidence: 72%

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Ionizing Radiation-Induced Extracellular Vesicle Release Promotes AKT-Associated Survival Response in SH-SY5Y Neuroblastoma Cells

Tortolici

Vumbaca

Incocciati

et al. 2021

Cells

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Radiation therapy is one of the most effective methods of tumor eradication; however, in some forms of neuroblastoma, radiation can increase the risk of secondary neoplasms, due to the ability of irradiated cells to transmit pro-survival signals to non-irradiated cells through vesicle secretion. The aims of this study were to characterize the vesicles released by the human neuroblastoma cell line SH-SY5Y following X-ray radiations and their ability to increase invasiveness in non-irradiated SH-SY5Y cells. We first purified the extracellular vesicles released by the SH-SY5Y cells following X-rays, and then determined their total amount, dimensions, membrane protein composition, and cellular uptake. We also examined the effects of these extracellular vesicles on viability, migration, and DNA damage in recipient SH-SY5Y cells. We found that exposure to X-rays increased the release of extracellular vesicles and altered their protein composition. These vesicles were readily uptaken by non-irradiated cells, inducing an increase in viability, migration, and radio-resistance. The same results were obtained in an MYCN-amplified SK-N-BE cell line. Our study demonstrates that vesicles released from irradiated neuroblastoma cells stimulate proliferation and invasiveness that correlate with the epithelial to mesenchymal transition in non-irradiated cells. Moreover, our results suggest that, at least in neuroblastomas, targeting the extracellular vesicles may represent a novel therapeutic approach to counteract the side effects associated with radiotherapy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 72%

See 1 more Smart Citation

Ionizing Radiation-Induced Extracellular Vesicle Release Promotes AKT-Associated Survival Response in SH-SY5Y Neuroblastoma Cells

Tortolici

Vumbaca

Incocciati

et al. 2021

Cells

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“…The zeta potential distribution of exosomes secreted from normal prostate cells and prostate cancer cells showed a positive shift after removing sialic acids by the treatment with sialidase [51]. In another study, the effect of the irradiation on the exosomal suspension was observed as a shift of the negative zeta potential toward more positive values [60]. Since these exosomes were released by neuroblastoma cells, we suggest that this effect can result from the degradation of polySia chains and/or the detachment of the polySia from the exosomal membrane protein, NCAM.…”

Section: Discussionmentioning

confidence: 99%

Exosome‐associated polysialic acid modulates membrane potentials, membrane thermotropic properties, and raft‐dependent interactions between vesicles

et al. 2020

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In mammals, polysialic acid (polySia) attached to a small number of transmembrane protein carriers occurs on the surface of plasma membranes of neural, cancer, immune, and placental trophoblast cells. Here, our goal was to demonstrate the presence of polySia on exosomes and its effect on membrane properties. We isolated exosomes and found that polysialylated exosomes in fetal bovine serum originate mostly from placental trophoblasts, while in calf bovine serum, they originate from immune cells. Enzymatic removal of polySia chains from the exosomal surface makes the membrane surface potential more positive, transmembrane potential more negative, and reduces the activation energy for membrane anisotropy changes. We demonstrate for the first time that exosomes could interact through polySia-raft interactions. We suggest that polysialylation of exosomal membrane can have a thermo-protecting effect and can modulate exosome-plasma membrane interactions.Exosomes are small extracellular vesicles (EVs), 50 Ä 150 nm in diameter, that originate from the endosome and are released from cells when multivesicular bodies (MVBs) containing intraluminal vesicles (ILVs) fuse with the plasma membrane. Microvesicles (100 Ä 1000 nm in diameter) are larger than exosomes and are released from cells through blebbing (budding out) and fission of the plasma membrane. There is a small overlap of exosomes and microvesicles concerning their size; however, vesicles within the diameter range of 50 Ä 100 nm are considered as exosomes. In addition to exosomes and microvesicles, there are also other nonvesicular nanoparticles in the body fluids, for example, lipoproteins and their complexes [1]. Exosomes are mainly recovered after high-speed ultracentrifugation, but such preparations may include the smallest non-EV structures, for example, high-density lipoprotein (5 Ä 35 nm in diameter) [1]. However, lipoproteins can be separated from exosomes by gel filtration method using appropriate matrix [2,3]. Exosomes are involved in mammalian pathophysiology including cancer, immune responses, cardiovascular diseases, regeneration, and stem cell-based therapy (reviewed in ref. [4]). Exosomes in cancer seem to be Abbreviations ANS, 8-anilino-1-naphthalenesulfonic acid ammonium salt

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“…Size distribution of the samples have been determined by NNLS analysis of DLS correlation functions within the framework of Mie. To get number distribution, we considered the vesicle model implemented in the Malvern NanoZS Research Software, with a refractive index equal to 1.4, as reported in the literature for different large vesicles [40,41].…”

Section: Dls Measurementsmentioning

confidence: 99%

Characterization of the Skeletal Muscle Secretome Reveals a Role for Extracellular Vesicles and IL1α/IL1β in Restricting Fibro/Adipogenic Progenitor Adipogenesis

et al. 2021

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Repeated mechanical stress causes injuries in the adult skeletal muscle that need to be repaired. Although muscle regeneration is a highly efficient process, it fails in some pathological conditions, compromising tissue functionality. This may be caused by aberrant cell–cell communication, resulting in the deposition of fibrotic and adipose infiltrates. Here, we investigate in vivo changes in the profile of skeletal muscle secretome during the regeneration process to suggest new targetable regulatory circuits whose failure may lead to tissue degeneration in pathological conditions. We describe the kinetic variation of expression levels of 76 secreted proteins during the regeneration process. In addition, we profile the gene expression of immune cells, endothelial cells, satellite cells, and fibro-adipogenic progenitors. This analysis allowed us to annotate each cell-type with the cytokines and receptors they have the potential to synthetize, thus making it possible to draw a cell–cell interaction map. We next selected 12 cytokines whose receptors are expressed in FAPs and tested their ability to modulate FAP adipogenesis and proliferation. We observed that IL1α and IL1β potently inhibit FAP adipogenesis, while EGF and BTC notably promote FAP proliferation. In addition, we characterized the cross-talk mediated by extracellular vesicles (EVs). We first monitored the modulation of muscle EV cargo during tissue regeneration. Using a single-vesicle flow cytometry approach, we observed that EVs differentially affect the uptake of RNA and proteins into their lumen. We also investigated the EV capability to interact with SCs and FAPs and to modulate their proliferation and differentiation. We conclude that both cytokines and EVs secreted during muscle regeneration have the potential to modulate adipogenic differentiation of FAPs. The results of our approach provide a system-wide picture of mechanisms that control cell fate during the regeneration process in the muscle niche.

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Effect of the irradiation on Neuroblastoma-derived microvesicles: A physical and biological investigation

Cited by 7 publications

References 35 publications

Ionizing Radiation-Induced Extracellular Vesicle Release Promotes AKT-Associated Survival Response in SH-SY5Y Neuroblastoma Cells

Ionizing Radiation-Induced Extracellular Vesicle Release Promotes AKT-Associated Survival Response in SH-SY5Y Neuroblastoma Cells

Exosome‐associated polysialic acid modulates membrane potentials, membrane thermotropic properties, and raft‐dependent interactions between vesicles

Characterization of the Skeletal Muscle Secretome Reveals a Role for Extracellular Vesicles and IL1α/IL1β in Restricting Fibro/Adipogenic Progenitor Adipogenesis

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