Effect of the initial glycerol concentration in the medium on the xanthan biosynthesis

Rončević, Zorana; Bajic, Z Bojana; Grahovac, Jovana; Dodic, N Sinisa; Dodic, M Jelena

doi:10.2298/apt1445239r

Cited by 9 publications

(9 citation statements)

References 12 publications

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“…The highest values of this parameter were obtained in the experiments where the media with the lowest examined glycerol concentrations were used, and vice versa indicating that an increase of the carbon source content in the cultivation medium negatively affects the xanthan quality. Also, it can be noted that the values of the molecular weight are in expected ranges for such a level of biopolymer purity and in agreement with a previous study (Rončević et al 2014).…”

Section: Resultssupporting

confidence: 92%

Optimisation of xanthan production on glycerol-based medium using response surface methodology

Rončević

Bajić

Vlajkov

et al. 2020

Braz. J. Chem. Eng.

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Xanthan is one of the most important biopolymers characterised by a high commercial value. Since the availability of essential nutrients influences its biosynthesis, the aim of this study was the optimisation of carbon, organic and inorganic nitrogen content in glycerol-based medium employing response surface methodology. The cultivation of strain Xanthomonas campestris ATCC 13951 was carried out under appropriate conditions on media with glycerol, peptone and ammonium-nitrate formulated according to Box-Behnken design (3 3), while the desirability function approach was used for determination of optimal nutrient levels. The final model predicts that the maximal amount of xanthan (12.95 g/L) is produced when the initial contents of glycerol, peptone and ammonium-nitrate in the medium are 32.96 g/L, 0.55 g/L and 0.73 g/L, respectively. To minimize the residual nutrient content and therefore the costs of effluent processing, additional optimisation was performed. In order to validate the optimisation model developed and examine the bioprocess success with crude glycerol as the sole carbon source, additional experiments were performed. The results represent reliable information for further investigations.

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Section: Resultssupporting

confidence: 92%

Optimisation of xanthan production on glycerol-based medium using response surface methodology

Rončević

Bajić

Vlajkov

et al. 2020

Braz. J. Chem. Eng.

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“…Glycerol content in cultivation media was 20.00 g/L. This concentration was selected based on the results from the previous study, conducted by the authors [22]. The cultivation medium also contained yeast extract (3.0 g/L), (NH 4 ) 2 SO 4 (1.5 g/L), K 2 HPO 4 (3.0 g/L) and MgSO 4 •7H 2 O (0.3 g/L).…”

Section: Cultivation Mediamentioning

confidence: 99%

Screening of Local Wild Xanthomonas Species for Xanthan Production on Crude Glycerol-based Medium

Zahović

Dodić²,

Grahovac³

et al. 2022

Period. Polytech. Chem. Eng.

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In this study, the effect of cultivation time on xanthan biosynthesis by different Xanthomonas campestris and Xanthomonas euvesicatoria strains, isolated from crucifers and pepper leaves, respectively, was examined. Xanthan was produced by submerged cultivation on crude glycerol-based medium at a laboratory level under aerobic conditions at 30 °C and 150 rpm for 168 h and 240 h. Bioprocess efficacy was estimated based on the xanthan concentration in media at the end of bioprocess and its average molecular weight. According to the obtained results, Xanthomonas strains have statistically significant effect on xanthan concentration in cultivation media when biosynthesis is performed by X. euvesicatoria strains, and cultivation time has significant effect on this parameter only when bioprocess is performed by X.campestris strains. The combination of Xanthomonas strains and cultivation time has a statistically significant effect on xanthan concentration in medium for both groups of isolates. The obtained results show that all applied Xanthomonas strains and cultivation time as well as their combination have statistically significant effect on average molecular weight of xanthan produced in applied experimental conditions. It is found that X. euvesicatoria strains produce higher amount of xanthan in a shorter period of time (168 h) when compared to the X.campestris strains. Xanthan of higher average molecular weight was produced when cultivation of both groups of isolates was performed for 240 h in applied experimental conditions. Results obtained in this research suggest that X. euvesicatoria strains have the greatest potential for application in biotechnological production of xanthan on crude glycerol-based medium.

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“…The primary structure of xanthan is composed of repeating units consisting of two glucose units, two mannose units and one glucuronic acid unit in the molar ratio 2.8:2.0:2.0. Xanthan molecular weight distribution depends on the association between structural chains and it ranges from around 2•10 5 to 5•10 7 g/moL (6,7). 174 Many types of bacteria of the genus Xanthomonas such as Xanthomonas campestris, Xanthomonas phaseoli, Xanthomonas malvacearum and Xanthomonas carotae have the ability to biosynthesize xanthan, but Xanthomonas campestris is the most widely used for its production (8).…”

Section: Introductionmentioning

confidence: 99%

The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium

et al. 2021

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In this study, the influence of cultivation time on xanthan biosynthesis by different Xanthomonas strains, reference strain and crucifers? and pepper leaves? isolates, was examined. Biopolymer was produced by submerged cultivation of fourteen producing strains on medium with glycerol as a sole carbon source. Each experiment was performed at a laboratory level under aerobic conditions at 30?C and 150 rpm for 168 h and 240 h. Bioprocess efficacy was estimated based on the xanthan quantity and its average molecular weight that was selected as quality parameter. According to the obtained results, it can be concluded that all applied strains have a statistically significant effect on xanthan concentration in medium and on its average molecular weight, while cultivation time significantly affect the bioprocess efficacy only when biosynthesis is performed by Xanthomonas strains isolated from crucifers. Further, when only Xanthomonas strains isolated from crucifers are observed, statistically obtained data suggest that the largest amount of the best quality xanthan in applied experimental conditions can be accomplished by the cultivation of CB strain for 240 h. On the other hand, when it comes to Xanthomonas strains isolated from pepper leaves, the highest productivity is shown by PL 2, PL 4 and PL 5 strains regardless of the cultivation time, while the PL 3 strain was responsible for the synthesis of biopolymers with the highest average molecular weight. The results obtained in this study represent valuable information for development of biotechnological process for xanthan production on glycerol containing media using new producing strain.

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Effect of the initial glycerol concentration in the medium on the xanthan biosynthesis

Cited by 9 publications

References 12 publications

Optimisation of xanthan production on glycerol-based medium using response surface methodology

Optimisation of xanthan production on glycerol-based medium using response surface methodology

Screening of Local Wild Xanthomonas Species for Xanthan Production on Crude Glycerol-based Medium

The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium

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