Effect of the ingestion of a dietary product containing Lactobacillus johnsonii La1 on Helicobacter pylori colonization in children

Cruchet, Sylvia; Obregón, María Clara; Salazar, Gabriela; Diaz, Eric; Gotteland, Martín

doi:10.1016/s0899-9007(03)00109-6

Cited by 143 publications

(96 citation statements)

References 36 publications

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“…However, the metabolic fate of orally ingested dead lactobacilli remains open. In vivo tests in humans aimed at the investigation of the digestion of heatkilled lactobacilli are of great interest with respect to some important findings of these probiotic bacteria (Simakachorn et al, 2000;Cruchet et al, 2003;Xiao et al, 2003). Therefore, in the present study we investigated the metabolic fate of heat-killed 15 N-L.casei after oral administration in correlation to the OCTT.…”

Section: Discussionmentioning

confidence: 99%

“…For development of potential health benefits to the consumer, the consumed yoghurt bacteria need to be viable. Nevertheless, comparisons have been made between viable and dead probiotic bacteria reported in various studies assessing the clinical efficacy of heat-killed lactobacilli, for example, in the treatment of diarrhoea (Xiao et al, 2003), in the decrease of Helicobacter pylori colonisation (Cruchet et al, 2003) or in the oral rehydration therapy (Simakachorn et al, 2000). However, the metabolic fate of dead lactobacilli remained unknown.…”

Section: Introductionmentioning

confidence: 99%

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15N-excretion of heat-killed Lactobacillus casei in humans

Wutzke

Sattinger

2006

Eur J Clin Nutr

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Objective: In the present study, Lactobacillus casei (L. casei) labelled with 15 N was used to follow the metabolic fate of orally administered heat-killed 15 N-labelled L. casei ( 15 N-L.casei) in humans. Design: Experimental study. Setting: University of Rostock, Children's Hospital, Research Laboratory. Subjects: Twelve healthy adults aged 23-32 years. Intervention: The subjects received 36 mg/kg body weight heat-killed 15 N-L.casei and 500 mg Lactose-[ 13 C]ureide together with breakfast. Expired air samples were taken over 14 h, whereas urine and faeces were collected over 2 days. A blood sample was taken after 2 h. 13 C-and 15 N-enrichments were measured by isotope ratio mass spectrometry (SerCon, UK). Results: The orocaecal transit time (OCTT) was reached after 4.1 h. The urinary 15 N-excretion was 9.3% of the ingested dose, whereas the faecal excretion was 65.1% of the ingested dose. After 2 h, 15 N-enrichment of supernatant, fibrinogen, and plasma protein precipitate amounted to 254, 11, and 2 p.p.m. excess, respectively. Conclusions: In comparison to the OCTT of 4.1 h, 15 N-enrichment in urinary ammonia and urinary total nitrogen already began to rise 30 min after 15 N-L.casei ingestion, indicating that 15 N-L.casei is rapidly digested in the small bowel. This is confirmed by 15 N-enrichments of blood plasma fractions. The ingestion of heat-killed 15 N-L.casei led to a total excretion of 74.4% of the ingested 15 N-dose.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

15N-excretion of heat-killed Lactobacillus casei in humans

Wutzke

Sattinger

2006

Eur J Clin Nutr

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“…30 The effect of L. johnsonii La1 was further explored in asymptomatic infected school children by Cruchet et al in Chile. 48 In a double blind, randomized, controlled clinical trial, 252 of 326 children (77.3%) 5-16 years of age and H. pylori-positive by 13 C-UBT were allocated to one of five groups. Two groups received a product containing live L. johnsonii La1 or L. paracasei ST11 at concentrations >10 7 CFU/mL every day for 4 weeks; two other groups received the product but with heat-killed La1 or ST11 while the fifth group received the yogurt vehicle as a negative control.…”

Section: Utilization Of Probiotics In Association With Antibiotics Inmentioning

confidence: 99%

Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori?

Gotteland

Brunser

Cruchet

2006

Aliment Pharmacol Ther

221

168

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SUMMARYHelicobacter pylori is a highly prevalent pathogen considered as an aetiological factor for gastroduodenal ulcers, and a risk factor for gastric adenocarcinoma and lymphoma in humans. Most subjects colonized by this micro-organism are asymptomatic and remain untreated. In symptomatic patients, the antibiotic treatment has a high cost and is not 100% effective because of resistance to antibiotics and to moderate patient compliance. This review discusses the role of probiotics as alternative solutions to assist in the control of H. pylori colonization in atrisk populations.The evidence that some strains of Lactobacillus and Bifidobacterium are able to inhibit H. pylori growth through the release of bacteriocins or organic acids, and may also decrease its adhesion to epithelial cells, is reviewed. In addition, probiotics have a possible role in the stabilization of the gastric barrier function and the decrease of mucosal inflammation. Other aspects that are considered are the contribution of probiotics to the healing of the gastric mucosa linked to their antioxidant and anti-inflammatory properties.Clinical trials in colonized adults and children are reviewed, and suggest that probiotics do not eradicate H. pylori but maintain lower levels of this pathogen in the stomach; in combination with antibiotics, probiotics may increase eradication rate and/or decrease adverse effects. Papers suggesting similar effects on H. pylori by foodstuffs such as berry juice and some milk proteins are quoted. Regular intake of these and other dietary products might constitute a low-cost, large-scale alternative solution applicable for populations at-risk for H. pylori colonization.

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“…However, the metabolic fate of orally ingested dead La1 remained open. In vivo tests in humans aimed at the investigation of the digestion of heat-killed lactobacilli are of great interest with respect to some important findings of these probiotic bacteria (Simakachorn et al, 2000;Cruchet et al, 2003;Xiao et al, 2003). Thus, in a subsequent study we investigated the metabolic fate of heat-killed 15 N-labelled (Wutzke and Sattinger, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…For development of potential health benefits to the consumer, the consumed La1 naturally needs to be viable. Nevertheless, comparisons have been made between viable and dead lactobacilli reported in various studies assessing the clinical efficacy of heat-killed lactobacilli, for example, in the treatment of diarrhoea, in the decrease of Helicobacter pylori colonization or in the oral rehydration therapy (Simakachorn et al, 2000;Cruchet et al, 2003;Xiao et al, 2003). However, the metabolic fate of dead lactobacilli remains unknown.…”

Section: Introductionmentioning

confidence: 99%

The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans

2007

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Objective: In this study, heat-killed Lactobacillus johnsonii (La1), doubly labelled with 13 C and 15 N (hk-dlLa1), was used to follow the metabolic fate after oral administration in humans. Design: Experimental study. Setting: University of Rostock, Children's Hospital, Research Laboratory. Subjects: Ten healthy adults aged 23-26 years. Intervention: The subjects received 74.6 mg/kg body weight hk-dlLa1 and 10 g a-D-raffinose together with breakfast. A sample of venous blood was taken after 2 h. Expired air samples were taken over 14 h, whereas urine and faeces were collected over a period of 48 h.13 C-and 15 N-enrichments were measured by isotope ratio mass spectrometry. Hydrogen concentrations were measured by electrochemical detection. Results: The orocaecal transit time (OCTT) was reached after 3.4 h. After 2 h, 13 C-and 15 N-enrichment of fibrinogen amounted to 2 and 25 p.p.m. excess, respectively. The 13 CO 2 -exhalation amounted to 9.2% of the ingested dose. The urinary excretion of 13 C and 15 N was 2.1 and 10.4% of the ingested dose, respectively, whereas the faecal excretion was 47.9 and 43.7% of the ingested dose, respectively. Conclusions: In comparison to OCTT of 3.4 h, both stable isotopes appear after 30 min in breath and urine, indicating that hk-dlLa1 is rapidly digested in the small bowel before reaching the caecum. This is confirmed by 13 C-and 15 N-enrichments of blood plasma fractions. The ingestion of hk-dlLa1 led to a 13 C-and 15 N-excretion of 59.2 and 54.1% of the ingested dose, respectively, of both stable isotopes.

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Effect of the ingestion of a dietary product containing Lactobacillus johnsonii La1 on Helicobacter pylori colonization in children

Cited by 143 publications

References 36 publications

15N-excretion of heat-killed Lactobacillus casei in humans

15N-excretion of heat-killed Lactobacillus casei in humans

Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori?

The metabolic fate of doubly stable isotope labelled heat-killed Lactobacillus johnsonii in humans

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