Effect of the <i>Penicillium chrysogenum</i> antifungal protein (PAF) on barley powdery mildew and wheat leaf rust pathogens

Barna, B.; Leiter, Éva; Hegedűs, Nikoletta; Bı́ró, Tamás; Pócsi, István

doi:10.1002/jobm.200800197

Cited by 26 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The germ-tubes, if present, were truncated and many showed abnormal growth. The results support Barna et al (2008) who showed the antifungal peptide PAF from Penicillium chrysogenum had a strong inhibitory effect on wheat leaf rust spores, including truncated and abnormal germ tubs (when present).…”

Section: Discussionsupporting

confidence: 85%

Stability of puroindoline peptides and effects on wheat rust

Alfred

Palombo

Panozzo³

et al. 2013

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Peptides modelled on the tryptophan rich domain of puroindolines and the related grain softness protein-1 have a broad range of antibacterial and antifungal activities. With the aims of further investigating the activities of these antimicrobial peptides we studied their activity against wheat rust diseases and environmental stability. PINA-based peptides were found to have high pH and thermal stability in addition to being stable over long periods at room temperature. These properties could make them excellent candidates as preservatives in food. PuroA, Pina-R39G and PuroB peptides adversely affected the morphology of the stripe rust spores (Puccinia striiformis f. sp. tritici), while PuroA and PuroB showed moderate inhibition of their germination. Additionally, GSP-5D reduced the germination of leaf rust spores (P. triticina). PuroA and PuroB sprayed onto stripe rust infected plants effected a moderate reduction in the number of stripe rust uredinia on wheat seedlings, as did PuroB sprayed onto the seedlings and allowed to coat the leaves for 5 day prior to spore infection. The results suggest that the presence of the PIN-based peptides may lower frequency of initial infection foci.

show abstract

Section: Discussionsupporting

confidence: 85%

Stability of puroindoline peptides and effects on wheat rust

Alfred

Palombo

Panozzo³

et al. 2013

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…PAF represents a promising bio-molecule for novel antifungal drug development as it is stable against proteolytic degradation, thermo-resistant and active within a broad pH range 9 , and shows no cytotoxic effects in vitro 10 or in vivo 11 , 12 . Furthermore, PAF could serve as a potential bio-pesticide in agriculture to impede infection by plant-pathogenic fungi 13 .…”

Section: Introductionmentioning

confidence: 99%

New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176

Huber

Hajdu

Bratschun-Khan

et al. 2018

Sci Rep

160

View full text Add to dashboard Cite

Small, cysteine-rich and cationic proteins with antimicrobial activity are produced by diverse organisms of all kingdoms and represent promising molecules for drug development. The ancestor of all industrial penicillin producing strains, the ascomycete Penicillium chryosgenum Q176, secretes the extensively studied antifungal protein PAF. However, the genome of this strain harbours at least two more genes that code for other small, cysteine-rich and cationic proteins with potential antifungal activity. In this study, we characterized the pafB gene product that shows high similarity to PgAFP from P. chrysogenum R42C. Although abundant and timely regulated pafB gene transcripts were detected, we could not identify PAFB in the culture broth of P. chrysogenum Q176. Therefore, we applied a P. chrysogenum-based expression system to produce sufficient amounts of recombinant PAFB to address unanswered questions concerning the structure and antimicrobial function. Nuclear magnetic resonance (NMR)-based analyses revealed a compact β-folded structure, comprising five β-strands connected by four solvent exposed and flexible loops and an "abcabc" disulphide bond pattern. We identified PAFB as an inhibitor of growth of human pathogenic moulds and yeasts. Furthermore, we document for the first time an anti-viral activity for two members of the small, cysteine-rich and cationic protein group from ascomycetes.The increasing incidence of fatal microbial infections due to the development of resistance against licensed antimicrobial drugs raises a strong demand for new antimicrobial treatment strategies. Filamentous ascomycetes are a rich source of antimicrobial bio-molecules that have the potential for wide application in medicine and agriculture to prevent and treat microbial infections 1 . As such, the industrially relevant fungus Penicillium chrysogenum is not only a well-known producer of the β-lactam antibiotic penicillin, but also secretes small, cysteine-rich and cationic proteins with antimicrobial activity. P. chrysogenum is an ideal producer of bio-products with beneficial potential to mankind as it is fermentable and bulk production is easy and cheap 2 . Most importantly, it is recognized as a "safe organism" by the US Food and Drug Administration.The P. chrysogenum strain Q176 is the ancestor of all industrial strains used for penicillin production today 2,3 and of the strains Wisconsin 54-1255 4 and P2niaD18 5 , whose genomes were sequenced and are publicly available. P. chrysogenum Q176 secretes the antifungal protein PAF whose structural and functional properties have been extensively studied [6][7][8] . PAF represents a promising bio-molecule for novel antifungal drug development as

show abstract

“…tritici) and millet rust (P. substriata) was reduced significantly in transgenic wheat and millet plants that expressed afp (antifungal protein from Aspergillus giganteus) within the apoplast, relative to wild-type control plants (Girgi et al, 2006;Oldach et al, 2001). Other studies have reported that foliar applications of plant extracts derived from Tulbaghia violacea and Agapanthus africanus reduced the pustule number and density of leaf rust on barley leaves (Cawood et al, 2010;Barna et al, 2008). However, the active constituents responsible for control remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of cereal rust fungi by both class I and II defensins derived from the flowers of Nicotiana alata

Dracatos

Weerden

Carroll

et al. 2013

Molecular Plant Pathology

View full text Add to dashboard Cite

Defensins are a large family of small, cysteine-rich, basic proteins, produced by most plants and plant tissues. They have a primary function in defence against fungal disease, although other functions have been described. This study reports the isolation and characterization of a class I secreted defensin (NaD2) from the flowers of Nicotiana alata, and compares its antifungal activity with the class II defensin (NaD1) from N. alata flowers, which is stored in the vacuole. NaD2, like all other class I defensins, lacks the C-terminal pro-peptide (CTPP) characteristic of class II defensins. NaD2 is most closely related to Nt-thionin from N. tabacum (96% identical) and shares 81% identity with MtDef4 from alfalfa. The concentration required to inhibit in vitro fungal growth by 50% (IC50 ) was assessed for both NaD1 and NaD2 for the biotrophic basidiomycete fungi Puccinia coronata f. sp. avenae (Pca) and P. sorghi (Ps), the necrotrophic pathogenic ascomycetes Fusarium oxysporum f. sp. vasinfectum (Fov), F. graminearum (Fgr), Verticillium dahliae (Vd) and Thielaviopsis basicola (Tb), and the saprobe Aspergillus nidulans. NaD1 was a more potent antifungal molecule than NaD2 against both the biotrophic and necrotrophic fungal pathogens tested. NaD2 was 5-10 times less effective at killing necrotrophs, but only two-fold less effective on Puccinia species. A new procedure for testing antifungal proteins is described in this study which is applicable to pathogens with spores that are not amenable to liquid culture, such as rust pathogens. Rusts are the most damaging fungal pathogens of many agronomically important crop species (wheat, barley, oats and soybean). NaD1 and NaD2 inhibited urediniospore germination, germ tube growth and germ tube differentiation (appressoria induction) of both Puccinia species tested. NaD1 and NaD2 were fungicidal on Puccinia species and produced stunted germ tubes with a granular cytoplasm. When NaD1 and NaD2 were sprayed onto susceptible oat plants prior to the plants being inoculated with crown rust, they reduced the number of pustules per leaf area, as well as the amount of chlorosis induced by infection. Similar to observations in vitro, NaD1 was more effective as an antifungal control agent than NaD2. Further investigation revealed that both NaD1 and NaD2 permeabilized the plasma membranes of Puccinia spp. This study provides evidence that both secreted (NaD2) and nonsecreted (NaD1) defensins may be useful for broad-spectrum resistance to pathogens.

show abstract

Effect of the Penicillium chrysogenum antifungal protein (PAF) on barley powdery mildew and wheat leaf rust pathogens

Cited by 26 publications

References 20 publications

Stability of puroindoline peptides and effects on wheat rust

Stability of puroindoline peptides and effects on wheat rust

New Antimicrobial Potential and Structural Properties of PAFB: A Cationic, Cysteine-Rich Protein from Penicillium chrysogenum Q176

Inhibition of cereal rust fungi by both class I and II defensins derived from the flowers of Nicotiana alata

Contact Info

Product

Resources

About